Galectin-9 Antibody [Unconjugated]

Images

 
Galectin‑9 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with Calcium Ionomycin and PMA using Goat Anti-Human Galectin‑9 Antigen Affinity-purified Polyclonal Antibody ...read more
Western blot shows conditioned media from HT‑29 human colon adenocarcinoma cell line, human peripheral blood lymphocytes (PBL), and human dendritic cells untreated (-) or treated (+) with 10 µg/mL PHA for 6 days. ...read more
Galectin‑9 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human Galectin‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) at 0.3 µg/mL for 1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Galectin-9 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Galectin-9
Specificity
Detects human Galectin-9 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Galectin-3 and rhGalectin-4 is observed and less than 2% cross-reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-7, and rhGalectin-8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LGALS9
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 0.3-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Control
Galectin-9 Knockout A549 Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
AF2045 in the following applications:

Publications
Read Publications using
AF2045 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-9 Antibody [Unconjugated]

  • Ecalectin
  • GAL9
  • gal-9
  • galectin 9
  • Galectin9
  • Galectin-9
  • HUAT
  • lectin, galactoside-binding, soluble, 9
  • LGALS9
  • LGALS9A
  • MGC117375
  • MGC125973
  • MGC125974
  • Tumor antigen HOM-HD-21
  • urate transporter/channel protein

Background

Galectin-9 (also known as Ecalectin and UAT) is a 40 kDa beta -galactosidase binding lectin that belongs to a tandem repeat group of Galectins. Activated cells such as T cells, PMNs, astrocytes, fibroblasts and endothelial cells can express Galectin-9, but its release requires select MMPs. Human to mouse, there is 69% aa sequence identity for Galectin-9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Galectin-9 Antibody (AF2045)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow Cytometry, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(2)
IHC-Fr
(1)
IHC-P
(4)
Neutralization
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(12)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF2045 Applications Species
K Sideras, RA de Man, SM Harrington, WG Polak, G Zhou, HM Schutz, A Pedroza-Go, K Biermann, S Mancham, BE Hansen, R Bart Takke, AJ van Vuuren, Q Pan, JNM Ijzermans, S Sleijfer, D Sprengers, H Dong, J Kwekkeboom, MJ Bruno Circulating levels of PD-L1 and Galectin-9 are associated with patient survival in surgically treated Hepatocellular Carcinoma independent of their intra-tumoral expression levels Sci Rep, 2019;9(1):10677. 2019 [PMID: 31337865] (IHC-P, Human) IHC-P Human
X Zhang, D He, S Gao, Y Wei, L Wang Aspergillus�fumigatus enhances human NK cell activity by regulating M1 macrophage polarization Mol Med Rep, 2019;20(2):1241-1249. 2019 [PMID: 31173233] (Western Blot, Human) Western Blot Human
JT Schroeder, AP Bieneman Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen J. Immunol., 2017;0(0):. 2017 [PMID: 28652400] (Neutralization, Human) Neutralization Human
Sun J, Yang M, Ban Y, Gao W, Song B, Wang Y, Zhang Y, Shao Q, Kong B, Qu X Tim-3 Is Upregulated in NK Cells during Early Pregnancy and Inhibits NK Cytotoxicity toward Trophoblast in Galectin-9 Dependent Pathway. PLoS ONE, 2016;11(1):e0147186. 2016 [PMID: 26789128] (IHC-P, Human) IHC-P Human
Punt S, Thijssen V, Vrolijk J, de Kroon C, Gorter A, Jordanova E Galectin-1, -3 and -9 Expression and Clinical Significance in Squamous Cervical Cancer. PLoS ONE, 2015;10(6):e0129119. 2015 [PMID: 26066796] (IHC-P, Human) IHC-P Human
Leskela S, Serrano A, de la Fuente H, Rodriguez-Munoz A, Ramos-Levi A, Sampedro-Nunez M, Sanchez-Madrid F, Gonzalez-Amaro R, Marazuela M Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells. PLoS ONE, 2015;10(4):e0123938. 2015 [PMID: 25880730] (Flow Cytometry, Human) Flow Cytometry Human
Schulkens I, Heusschen R, van den Boogaart V, van Suylen R, Dingemans A, Griffioen A, Thijssen V Galectin expression profiling identifies galectin-1 and Galectin-9Delta5 as prognostic factors in stage I/II non-small cell lung cancer. PLoS ONE, 2014;9(9):e107988. 2014 [PMID: 25259711] (Western Blot, Human) Western Blot Human
McSharry B, Forbes S, Cao J, Avdic S, Machala E, Gottlieb D, Abendroth A, Slobedman B Human cytomegalovirus upregulates expression of the lectin galectin 9 via induction of beta interferon. J Virol, 2014;88(18):10990-4. 2014 [PMID: 25008927] (Western Blot, Human) Western Blot Human
Leitner, Judith, Rieger, Armin, Pickl, Winfried, Zlabinger, Gerhard, Grabmeier-Pfistershammer, Katharin, Steinberger, Peter TIM-3 does not act as a receptor for galectin-9. PLoS Pathog, 2013;9(3):e1003253. 2013 [PMID: 23555261] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Nebbia G, Peppa D, Schurich A, Khanna P, Singh H, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, Chinaleong J, Kennedy P, Maini M Upregulation of the tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection. PLoS ONE, 2012;7(10):e47648. 2012 [PMID: 23112829] (IHC-Fr, Human) IHC-Fr Human
Show All 13 Publications.

Review for Galectin-9 Antibody (AF2045) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2045:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Galectin-9 AF2045
Enlarge
5
reviewed by:
Anonymous
IHC-P Human 04/04/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Tested breast cancer tissue
SpeciesHuman
LotKNI0117011

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Biotin BAF2045

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
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Anonymous
04/04/2020
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol LGALS9