PD-1 Antibody [Unconjugated]

Images

 
Western blot shows lysate of human thymus tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) followed by HRP-conjugated Anti-Goat ...read more
Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 μg/mL PHA overnight were stained with Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human PD-1. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human PD‑1 Antigen ...read more
PD‑1 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) at 3 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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PD-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human PD-1
Leu25-Gln167
Accession # Q8IX89
Specificity
Detects human PD-1 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse PD‑1 and less than 0.2% cross-reactivity with recombinant human (rh) CD28, rhICOS, and rhCTLA‑4 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDCD1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human PD-1 Antibody (Catalog # AF1086)
ELISA Detection: Human PD-1 Biotinylated Antibody (Catalog # BAF1086)
Standard: Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD)
Reviewed Applications
Read 1 Review rated 4
using
AF1086 in the following applications:

Publications
Read Publications using
AF1086 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PD-1 Antibody [Unconjugated]

  • CD279 antigen
  • CD279
  • hPD-1
  • PD-1
  • PD1hPD-l
  • PDCD1
  • programmed cell death 1
  • programmed cell death protein 1
  • Protein PD-1
  • SLEB2

Background

Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or down-regulate T cell activation (CTLA-4 and PD-1) (2). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. In vitro, ligation of PD-1 inhibits TCR-mediated T-cell proliferation and production of IL-1, IL-4, IL-10, and IFN-gamma. In addition, PD-1 ligation also inhibits BCR mediated signaling. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases (2, 3).

Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2 (also known as B7-DC), have been identified as PD-1 ligands. Unlike other B7 family proteins, both
PD‑L1 and PD-L2 are expressed in a wide variety of normal tissues including heart, placenta, and activated spleens (4). The wide expression of PD-L1 and PD-L2 and the inhibitor effects on PD-1 ligation indicate that PD-1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases (2).

The human PD-1 gene encodes a 288 amino acid (aa) protein with a putative 20 aa signal peptide, a 148 aa extracellular region with one immunoglobulin-like V-type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immuno-receptor
tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 60% aa sequence identity (4).

  1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
  2. Nishimura, H. and T. Honjo (2001) Trends in Immunol. 22:265.
  3. Latchman, Y. et al. (2001) Nature Immun. 2:261.
  4. Carreno, B.M. and M. Collins (2002) Annu. Rev. Immunol. 20:29.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PD-1 Antibody (AF1086)(18)

We have publications tested in 5 confirmed species: Human, Mouse, Primate, Primate - Cercocebus torquatus (Sooty Mangabey), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 10 applications: ELISA Development, Flow, Func, IF, IHC, IHC - Paraffin embedded, IHC Fluorescence, IHC Paraffin-embedded, IHC-P, Neut.


Filter By Application
ELISA Development
(2)
Flow
(1)
Func
(2)
IF
(2)
IHC
(4)
IHC - Paraffin embedded
(1)
IHC Fluorescence
(1)
IHC Paraffin-embedded
(3)
IHC-P
(1)
Neut
(1)
All Applications
Filter By Species
Human
(8)
Mouse
(1)
Primate
(3)
Primate - Cercocebus torquatus (Sooty Mangabey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using AF1086 Applications Species
F Wang, B Li, Y Wei, Y Zhao, L Wang, P Zhang, J Yang, W He, H Chen, Z Jiao, Y Li Tumor-derived exosomes induce PD1+ macrophage population in human gastric cancer that promotes disease progression Oncogenesis, 2018;7(5):41. 2018 [PMID: 29799520] (Func,, Human) Func Human
W Fan, AJ Demers, Y Wan, Q Li Altered Ratio of T Follicular Helper Cells to T Follicular Regulatory Cells Correlates with Autoreactive Antibody Response in Simian Immunodeficiency Virus-Infected Rhesus Macaques J. Immunol., 2018;0(0):. 2018 [PMID: 29610141] (Flow,, Primate) Flow Primate
MA Rahman, KM McKinnon, TS Karpova, DA Ball, DJ Venzon, W Fan, G Kang, Q Li, M Robert-Gur Associations of Simian Immunodeficiency Virus (SIV)-Specific Follicular CD8+T Cells with Other Follicular T Cells Suggest Complex Contributions to SIV Viremia Control J. Immunol., 2018;0(0):. 2018 [PMID: 29507105] (IHC,, Primate) IHC Primate
D Amodio, N Cotugno, G Macchiarul, S Rocca, Y Dimopoulos, MR Castrucci, R De Vito, FM Tucci, AB McDermott, S Narpala, P Rossi, RA Koup, P Palma, C Petrovas Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination J. Immunol., 2017;0(0):. 2017 [PMID: 29237774] (IHC,, Human) IHC Human
Y Liu, Y Cheng, Y Xu, Z Wang, X Du, C Li, J Peng, L Gao, X Liang, C Ma Increased expression of programmed cell death protein 1 on NK cells inhibits NK-cell-mediated anti-tumor function and indicates poor prognosis in digestive cancers Oncogene, 2017;0(0):. 2017 [PMID: 28692048] (Func,, Human) Func Human
ELV Silveira, KA Rogers, S Gumber, P Amancha, P Xiao, SM Woollard, SN Byrareddy, MM Teixeira, F Villinger Immune Cell Dynamics in Rhesus Macaques Infected with a Brazilian Strain of Zika Virus J. Immunol., 2017;0(0):. 2017 [PMID: 28667164] (IHC,, Primate - Macaca mulatta (Rhesus Macaque)) IHC Primate - Macaca mulatta (Rhesus Macaque)
P Martinez-M, K Tran, J Guenaga, G Lindgren, M Àdori, Y Feng, GE Phad, N Vázquez Be, S Bale, J Ingale, V Dubrovskay, S O'Dell, L Pramanik, M Spångberg, M Corcoran, K Loré, JR Mascola, RT Wyatt, GB Karlsson H Particulate Array of Well-Ordered HIV Clade C Env Trimers Elicits Neutralizing Antibodies that Display a Unique V2 Cap Approach Immunity, 2017;46(5):804-817.e7. 2017 [PMID: 28514687] (IHC,, Primate) IHC Primate
Joseph M Obeid PD-L1, PD-L2 and PD-1 expression in metastatic melanoma: Correlation with tumor-infiltrating immune cells and clinical outcome Oncoimmunology, 2016;5(11):e1235107. 2016 [PMID: 27999753] (IHC-P,, Human) IHC-P Human
PT Nghiem, S Bhatia, EJ Lipson, RR Kudchadkar, NJ Miller, L Annamalai, S Berry, EK Chartash, A Daud, SP Fling, PA Friedlande, HM Kluger, HE Kohrt, L Lundgren, K Margolin, A Mitchell, T Olencki, DM Pardoll, SA Reddy, EM Shantha, WH Sharfman, E Sharon, She PD-1 Blockade with Pembrolizumab in Advanced Merkel-Cell Carcinoma N Engl J Med, 2016;0(0):. 2016 [PMID: 27093365] (IHC - Paraffin embedded,, Human) IHC - Paraffin embedded Human
Vargas-Inchaustegui D, Demers A, Shaw J, Kang G, Ball D, Tuero I, Musich T, Mohanram V, Demberg T, Karpova T, Li Q, Robert-Guroff M Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques. J Immunol, 2016;196(4):1700-10. 2016 [PMID: 26773147] (IHC Fluorescence,, Primate - Macaca mulatta (Rhesus Macaque)) IHC Fluorescence Primate - Macaca mulatta (Rhesus Macaque)
Show All 18 Publications.

Review for PD-1 Antibody (AF1086) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1086:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence PD-1 AF1086
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reviewed by:
Christopher England
IF Human 07/09/2016
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Summary

ApplicationImmunofluorescence
Sample TestedMouse salivary gland (one injected with human T cells and one with no human T cells)
SpeciesHuman
LotICA0714071

Blocking

Blocking Details10% Normal Donkey Serum, 0.25% Triton-X in PBS, 1 hr at room temperature

Primary Anitbody

Dilution Ratio1:200, 12 h, 4 degree Celcius, PBS w/0.25% Triton-X

Secondary Antibody

Secondary DescriptionDonkey anti-Goat DyLight 488
Secondary Manufacturer Cat#NBP-1-72843
Secondary Concentration1:4000

Details

Detection NotesConfocal microscopy, Nikon A1R with 488-nm laser
Fixation DetailsFrozen Sections, 4% Paraformaldehyde in PBS for 30 min at room temperature
Wash DescriptionPBS, 15 min, 3-times after primary and 3-times after secondary

Comments

CommentsThe antibody worked well. Since goat-derived antibodies are not the best, we were happy to see that this one worked well.

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FAQs for PD-1 Antibody (AF1086) (0)

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Blogs on PD-1.

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Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

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Recent Reviews

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Christopher England
07/09/2016
Application: IF
Species: Human

Bioinformatics

Gene Symbol PDCD1
Uniprot