PD-1 Antibody [Unconjugated]

Images

 
Western blot shows lysate of human thymus tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) followed by HRP-conjugated Anti-Goat ...read more
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human PD-1. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human PD‑1 Antigen ...read more
PD‑1 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) at 3 µg/mL overnight at 4 °C. Tissue ...read more
Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 μg/mL PHA overnight were stained with Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

PD-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human PD-1
Leu25-Gln167
Accession # Q8IX89
Specificity
Detects human PD-1 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse PD‑1 and less than 0.2% cross-reactivity with recombinant human (rh) CD28, rhICOS, and rhCTLA‑4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDCD1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.5-2 ug/mL
Application Notes
ELISA Capture: Human PD-1 Antibody (Catalog # AF1086)
ELISA Detection: Human PD-1 Biotinylated Antibody (Catalog # BAF1086)
Standard: Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD)
Reviewed Applications
Read 1 Review rated 4
using
AF1086 in the following applications:

Publications
Read Publications using
AF1086 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PD-1 Antibody [Unconjugated]

  • CD279 antigen
  • CD279
  • hPD-1
  • PD1
  • PD-1
  • PD1hPD-l
  • PDCD1
  • programmed cell death 1
  • programmed cell death protein 1
  • Protein PD-1
  • SLEB2

Background

Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or down-regulate T cell activation (CTLA-4 and PD-1) (2). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. In vitro, ligation of PD-1 inhibits TCR-mediated T-cell proliferation and production of IL-1, IL-4, IL-10, and IFN-gamma . In addition, PD-1 ligation also inhibits BCR mediated signaling. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases (2, 3).

Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2 (also known as B7-DC), have been identified as PD-1 ligands. Unlike other B7 family proteins, both
PD‑L1 and PD-L2 are expressed in a wide variety of normal tissues including heart, placenta, and activated spleens (4). The wide expression of PD-L1 and PD-L2 and the inhibitor effects on PD-1 ligation indicate that PD-1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases (2).

The human PD-1 gene encodes a 288 amino acid (aa) protein with a putative 20 aa signal peptide, a 148 aa extracellular region with one immunoglobulin-like V-type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immuno-receptor
tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 60% aa sequence identity (4).

  1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
  2. Nishimura, H. and T. Honjo (2001) Trends in Immunol. 22:265.
  3. Latchman, Y. et al. (2001) Nature Immun. 2:261.
  4. Carreno, B.M. and M. Collins (2002) Annu. Rev. Immunol. 20:29.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

7268-CT
Species: Hu
Applications: BA
NBP1-76769
Species: Hu, Mu, Rt
Applications: Dual ISH-IHC, ELISA, Flow, ICC/IF, IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, IP, KD, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
MAB1224
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
MAB140
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
202-IL
Species: Hu
Applications: BA
DY417
Species: Mu
Applications: ELISA
MAB342
Species: Hu
Applications: AgAct, WB
169-CS
Species: Hu
Applications: BA
NB100-39002
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
DR2A00
Species: Hu
Applications: ELISA
H00003669-M01
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
AF2365
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, IHC, KO, WB
NBP2-25208
Species: Hu, Mu, Rt
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
6507-IL/CF
Species: Hu
Applications: BA

Publications for PD-1 Antibody (AF1086)(30)

We have publications tested in 6 confirmed species: Human, Mouse, Feline, Primate, Primate - Cercocebus torquatus (Sooty Mangabey), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 9 applications: Bioassay, ELISA Development, Flow Cytometry, Functional Assay, ICC, IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
ELISA Development
(2)
Flow Cytometry
(2)
Functional Assay
(2)
ICC
(1)
IHC
(11)
IHC-P
(8)
Neutralization
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(17)
Mouse
(2)
Feline
(1)
Primate
(3)
Primate - Cercocebus torquatus (Sooty Mangabey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(6)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using AF1086 Applications Species
D Rafei-Sham, S Lehr, M Behrens, F Meiss Additive Intralesional Interleukin-2 Improves Progression-Free Survival in a Distinct Subgroup of Melanoma Patients with Prior Progression under Immunotherapy Cancers, 2022;14(3):. 2022 [PMID: 35158808] (IHC, Human) IHC Human
C Takasu, S Yamashita, Y Morine, K Yoshikawa, T Tokunaga, M Nishi, H Kashihara, T Yoshimoto, M Shimada The role of the immunoescape in colorectal cancer liver metastasis PLoS ONE, 2021;16(11):e0259940. 2021 [PMID: 34797860] (IHC, Human) IHC Human
C Glorieux, X Xia, YQ He, Y Hu, K Cremer, A Robert, J Liu, F Wang, J Ling, PJ Chiao, P Huang Regulation of PD-L1 expression in K-ras-driven cancers through ROS-mediated FGFR1 signaling Redox Biology, 2020;38(0):101780. 2020 [PMID: 33171331] (Western Blot, Mouse) Western Blot Mouse
C Takasu, M Nishi, K Yoshikawa, T Tokunaga, H Kashihara, T Yoshimoto, M Shimada Impact of sidedness of colorectal cancer on tumor immunity PLoS ONE, 2020;15(10):e0240408. 2020 [PMID: 33045001] (IHC, Human) IHC Human
R Deng, C Zuo, Y Li, B Xue, Z Xun, Y Guo, X Wang, Y Xu, R Tian, S Chen, Q Liu, J Chen, J Wang, X Huang, H Li, M Guo, X Wang, M Yang, Z Wu, J Wang, J Ma, J Hu, G Li, S Tang, Z Tu, H Ji, H Zhu The innate immune effector ISG12a promotes cancer immunity by suppressing the canonical Wnt/&amp;beta-catenin signaling pathway Cell. Mol. Immunol., 2020;0(0):. 2020 [PMID: 32963356] (Neutralization, Human) Neutralization Human
JE Eckel-Pass, TH Ho, DJ Serie, JC Cheville, R Houston Th, BA Costello, H Dong, ED Kwon, BC Leibovich, AS Parker Concordance of PD-1 and PD-L1 (B7-H1) in paired primary and metastatic clear cell renal cell carcinoma Cancer Med, 2019;0(0):. 2019 [PMID: 31829518] (IHC-P, Human) IHC-P Human
S Yang, J Wei, YH Cui, G Park, P Shah, Y Deng, AE Aplin, Z Lu, S Hwang, C He, YY He m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade Nat Commun, 2019;10(1):2782. 2019 [PMID: 31239444] (ICC, Human) ICC Human
N Taechangam, SS Iyer, NJ Walker, B Arzi, DL Borjesson Mechanisms utilized by feline adipose-derived mesenchymal stem cells to inhibit T lymphocyte proliferation Stem Cell Res Ther, 2019;10(1):188. 2019 [PMID: 31238978] (Flow Cytometry, Feline) Flow Cytometry Feline
S Li, JM Folkvord, KJ Kovacs, RK Wagstaff, G Mwakalundw, AK Rendahl, EG Rakasz, E Connick, PJ Skinner Low levels of SIV-specific CD8+ T cells in germinal centers characterizes acute SIV infection PLoS Pathog., 2019;15(3):e1007311. 2019 [PMID: 30897187] (IHC, Primate - Macaca mulatta (Rhesus Macaque)) IHC Primate - Macaca mulatta (Rhesus Macaque)
B Li, TN Song, FR Wang, C Yin, Z Li, JP Lin, YQ Meng, HM Feng, T Jing Tumor-derived exosomal HMGB1 promotes esophageal squamous cell carcinoma progression through inducing PD1+ TAM expansion Oncogenesis, 2019;8(3):17. 2019 [PMID: 30796203] (Bioassay, Human) Bioassay Human
Show All 30 Publications.

Review for PD-1 Antibody (AF1086) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1086:
Filter by Applications
Immunofluorescence
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence PD-1 AF1086
Enlarge
4
reviewed by:
Verified Customer
Immunofluorescence Human 07/09/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse salivary gland (one injected with human T cells and one with no human T cells)
SpeciesHuman
LotICA0714071

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PD-1 Antibody (AF1086) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PD-1 Products

Bioinformatics Tool for PD-1 Antibody (AF1086)

Discover related pathways, diseases and genes to PD-1 Antibody (AF1086). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PD-1 Antibody (AF1086)

Discover more about diseases related to PD-1 Antibody (AF1086).
 

Pathways for PD-1 Antibody (AF1086)

View related products by pathway.

PTMs for PD-1 Antibody (AF1086)

Learn more about PTMs related to PD-1 Antibody (AF1086).

Blogs on PD-1.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Synthetic Biotic Medicine as Immunotherapy Against Cancer: Evidence From Arginine-Producing Engineered Bacteria
By Jamshed Arslan, Pharm D, PhDWhat do nuts, dairy and red meat have in common? In addition to the fact that they are all edible, one of the answers is L-arginine. This amino acid improves T cell’s respons...  Read full blog post.

Thomson Reuters Predicts 2016 Nobel Prize Winners
Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Verified Customer
07/09/2016
Application: Immunofluorescence
Species: Human

Bioinformatics

Gene Symbol PDCD1
Uniprot