Reactivity | HuSpecies Glossary |
Applications | WB, Flow, IHC, B/N, CyTOF-ready, ELISA(Cap) |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Unconjugated |
Concentration | LYOPH |
Conjugate | Catalog # | Availability | Size | Price |
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Alexa Fluor 488 | FAB7115G | |||
Biotin | BAF1086 | |||
Phycoerythrin | FAB7115P | |||
Immunogen | Mouse myeloma cell line NS0-derived recombinant human PD-1 Leu25-Gln167 Accession # Q8IX89 |
Specificity | Detects human PD-1 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse PD‑1 and less than 0.2% cross-reactivity with recombinant human (rh) CD28, rhICOS, and rhCTLA‑4 is observed. |
Details of Functionality | ActivityAssaywCitation not found None None None None None |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | PDCD1 |
Purity Statement | Antigen Affinity-purified |
Endotoxin Note | <0.10 EU per 1 μg of the antibody by the LAL method. |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | ELISA Capture: Human PD-1 Antibody (Catalog # AF1086) ELISA Detection: Human PD-1 Biotinylated Antibody (Catalog # BAF1086) Standard: Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or down-regulate T cell activation (CTLA-4 and PD-1) (2). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. In vitro, ligation of PD-1 inhibits TCR-mediated T-cell proliferation and production of IL-1, IL-4, IL-10, and IFN-gamma. In addition, PD-1 ligation also inhibits BCR mediated signaling. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases (2, 3).
Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2 (also known as B7-DC), have been identified as PD-1 ligands. Unlike other B7 family proteins, both
PD‑L1 and PD-L2 are expressed in a wide variety of normal tissues including heart, placenta, and activated spleens (4). The wide expression of PD-L1 and PD-L2 and the inhibitor effects on PD-1 ligation indicate that PD-1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases (2).
The human PD-1 gene encodes a 288 amino acid (aa) protein with a putative 20 aa signal peptide, a 148 aa extracellular region with one immunoglobulin-like V-type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immuno-receptor
tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 60% aa sequence identity (4).
Images | Ratings | Applications | Species | Date | Details | ||||||||||||||||||||||||||
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reviewed by:
Christopher England |
IF | Human | 07/09/2016 |
Summary
Blocking
Primary Anitbody
Secondary Antibody
Details
Comments
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Secondary Antibodies |
Isotype Controls |
Alexa Fluor 488 | FAB7115G | |
Biotin | BAF1086 | |
Phycoerythrin | FAB7115P |
Diseases for PD-1 Antibody (AF1086)Discover more about diseases related to PD-1 Antibody (AF1086).
| Pathways for PD-1 Antibody (AF1086)View related products by pathway.
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PTMs for PD-1 Antibody (AF1086)Learn more about PTMs related to PD-1 Antibody (AF1086).
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