PD-1 Antibody [Unconjugated]


Western blot shows lysate of human thymus tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) followed by HRP-conjugated Anti-Goat ...read more
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human PD-1. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human PD‑1 Antigen ...read more
Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 μg/mL PHA overnight were stained with Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
PD‑1 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) at 3 µg/mL overnight at 4 °C. Tissue ...read more
PD-L1, PD-L2, PD-1, CD8, and CD4 expression in p16-positive and p16-negative HNSCC. PD-L1, PD-L2, PD-1, CD8, and CD4 expression was assessed in tumor biopsy tissue from five p16-positive and four p16-negative HNSCC ...read more
Modulation of soluble co-signaling molecules in kidney-transplanted patients over time.The levels of the soluble co-signaling molecules CD30, CD40, CD137, CD40L, PD-1 and PD-L1 were assayed by ELISA in serum samples of ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready, ELISA(Cap)

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PD-1 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human PD-1
Accession # Q8IX89
Detects human PD-1 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse PD‑1 and less than 0.2% cross-reactivity with recombinant human (rh) CD28, rhICOS, and rhCTLA‑4 is observed.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.5-2 ug/mL
Application Notes
ELISA Capture: Human PD-1 Antibody (Catalog # AF1086)
ELISA Detection: Human PD-1 Biotinylated Antibody (Catalog # BAF1086)
Standard: Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD)
Reviewed Applications
Read 1 Review rated 4
AF1086 in the following applications:

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AF1086 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PD-1 Antibody [Unconjugated]

  • CD279 antigen
  • CD279
  • hPD-1
  • PD1
  • PD-1
  • PD1hPD-l
  • PDCD1
  • programmed cell death 1
  • programmed cell death protein 1
  • Protein PD-1
  • SLEB2


Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or down-regulate T cell activation (CTLA-4 and PD-1) (2). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. In vitro, ligation of PD-1 inhibits TCR-mediated T-cell proliferation and production of IL-1, IL-4, IL-10, and IFN-gamma . In addition, PD-1 ligation also inhibits BCR mediated signaling. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases (2, 3).

Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2 (also known as B7-DC), have been identified as PD-1 ligands. Unlike other B7 family proteins, both
PD‑L1 and PD-L2 are expressed in a wide variety of normal tissues including heart, placenta, and activated spleens (4). The wide expression of PD-L1 and PD-L2 and the inhibitor effects on PD-1 ligation indicate that PD-1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases (2).

The human PD-1 gene encodes a 288 amino acid (aa) protein with a putative 20 aa signal peptide, a 148 aa extracellular region with one immunoglobulin-like V-type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immuno-receptor
tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 60% aa sequence identity (4).

  1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
  2. Nishimura, H. and T. Honjo (2001) Trends in Immunol. 22:265.
  3. Latchman, Y. et al. (2001) Nature Immun. 2:261.
  4. Carreno, B.M. and M. Collins (2002) Annu. Rev. Immunol. 20:29.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PD-1 Antibody (AF1086)(34)

We have publications tested in 6 confirmed species: Human, Mouse, Feline, Primate, Primate - Cercocebus atys (Sooty Mangabey), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 9 applications: Bioassay, ELISA Development, Flow Cytometry, Functional Assay, ICC, IHC, IHC-P, Neutralization, Western Blot.

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Primate - Cercocebus atys (Sooty Mangabey)
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using AF1086 Applications Species
H�nze, J;Schulte-Herbr�ggen, J;Hofmann, R;Hegele, A; Modulation of immune checkpoint regulators in interferon ? induced urothelial carcinoma and activated T-lymphocyte cells by cytostatics Genes and immunity 2023-05-03 [PMID: 37138100] (Western Blot, Human) Western Blot Human
J Wu, R Zhu, Z Wang, X Chen, T Xu, Y Liu, M Song, J Jiang, Q Ma, Z Chen, Y Liu, X Wang, M Zhang, M Huang, N Ji Exosomes in malignant pleural effusion from lung cancer patients impaired the cytotoxicity of double-negative T cells Translational Oncology, 2022-10-14;27(0):101564. 2022-10-14 [PMID: 36252282] (Bioassay, Human) Bioassay Human
AG Goubet, L Lordello, C Alves Cost, I Peguillet, M Gazzano, MD Mbogning-F, C Thelemaque, C Lebacle, C Thibault, F Audenet, G Pignot, G Gravis, C Helissey, L Campedel, M Roupret, E Xylinas, I Ouzaid, A Dubuisson, M Mazzenga, C Flament, P Ly, V Marty, N Signolle, A Sauvat, T Sbarrato, M Filahi, C Davin, G Haddad, J Bou Khalil, C Bleriot, FX Danlos, G Dunsmore, K Mulder, A Silvin, T Raoult, B Archambaud, S Belhechmi, I Gomperts B, N Cayet, M Moya-Nilge, A Mallet, R Daillere, E Rouleau, C Radulescu, Y Allory, J Fieschi, M Rouanne, F Ginhoux, G Le Teuff, L Derosa, A Marabelle, J VAN Dorp, N VAN Dijk, MS van der He, B Besse, F Andre, M Merad, G Kroemer, JY Scoazec, L Zitvogel, Y Loriot Escherichia coli-specific CXCL13-producing TFH are associated with clinical efficacy of neoadjuvant PD-1 blockade against muscle-invasive bladder cancer Cancer Discovery, 2022-10-05;0(0):. 2022-10-05 [PMID: 35929803] (IHC, Human) IHC Human
S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis Nature Communications, 2022-06-03;13(1):3121. 2022-06-03 [PMID: 35660734] (Neutralization, Human) Neutralization Human
D Rafei-Sham, S Lehr, M Behrens, F Meiss Additive Intralesional Interleukin-2 Improves Progression-Free Survival in a Distinct Subgroup of Melanoma Patients with Prior Progression under Immunotherapy Cancers, 2022-01-21;14(3):. 2022-01-21 [PMID: 35158808] (IHC, Human) IHC Human
C Takasu, S Yamashita, Y Morine, K Yoshikawa, T Tokunaga, M Nishi, H Kashihara, T Yoshimoto, M Shimada The role of the immunoescape in colorectal cancer liver metastasis PLoS ONE, 2021-11-19;16(11):e0259940. 2021-11-19 [PMID: 34797860] (IHC, Human) IHC Human
C Glorieux, X Xia, YQ He, Y Hu, K Cremer, A Robert, J Liu, F Wang, J Ling, PJ Chiao, P Huang Regulation of PD-L1 expression in K-ras-driven cancers through ROS-mediated FGFR1 signaling Redox Biology, 2020-11-03;38(0):101780. 2020-11-03 [PMID: 33171331] (Western Blot, Mouse) Western Blot Mouse
C Takasu, M Nishi, K Yoshikawa, T Tokunaga, H Kashihara, T Yoshimoto, M Shimada Impact of sidedness of colorectal cancer on tumor immunity PLoS ONE, 2020-10-12;15(10):e0240408. 2020-10-12 [PMID: 33045001] (IHC, Human) IHC Human
R Deng, C Zuo, Y Li, B Xue, Z Xun, Y Guo, X Wang, Y Xu, R Tian, S Chen, Q Liu, J Chen, J Wang, X Huang, H Li, M Guo, X Wang, M Yang, Z Wu, J Wang, J Ma, J Hu, G Li, S Tang, Z Tu, H Ji, H Zhu The innate immune effector ISG12a promotes cancer immunity by suppressing the canonical Wnt/&amp;beta-catenin signaling pathway Cell. Mol. Immunol., 2020-09-22;0(0):. 2020-09-22 [PMID: 32963356] (Neutralization, Human) Neutralization Human
JE Eckel-Pass, TH Ho, DJ Serie, JC Cheville, R Houston Th, BA Costello, H Dong, ED Kwon, BC Leibovich, AS Parker Concordance of PD-1 and PD-L1 (B7-H1) in paired primary and metastatic clear cell renal cell carcinoma Cancer Med, 2019-12-12;0(0):. 2019-12-12 [PMID: 31829518] (IHC-P, Human) IHC-P Human
Show All 34 Publications.

Review for PD-1 Antibody (AF1086) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1086:
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Images Ratings Applications Species Date Details
Immunofluorescence PD-1 AF1086
reviewed by:
Christopher England
IF Human 07/09/2016


Sample TestedMouse salivary gland (one injected with human T cells and one with no human T cells)

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Recent Reviews


Christopher England
Application: IF
Species: Human


Gene Symbol PDCD1