TIM-1/KIM-1/HAVCR Antibody (511227)

• Reactivity: Rt
• Applications: ELISA(Cap)
• Clone: 511227
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TIM-1/KIM-1/HAVCR Antibody (511227) Summary

• Immunogen: Mouse Myeloma cell line NS0-derived recombinant rat TIM-1/KIM-1/HAVCR
  Ser18-Val238
  Accession # O54947
• Specificity: Detects rat TIM-1/KIM-1/HAVCR in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse (rm) TIM-1, rmTIM-3, rmTIM-4, recombinant human (rh) TIM-1, rhTIM-4, or rhNKp44 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HAVCR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Rat TIM-1/KIM-1/HAVCR Biotinylated Antibody (Catalog number BAF3689) Standard: Recombinant Rat TIM-1/KIM-1/HAVCR (Catalog number 3689-TM)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is covered by one or more of the following US Patents 7,300,652; 7,041,290; 6,664,385 and other US and foreign patents pending or issued. This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-1/KIM-1/HAVCR Antibody (511227)

• CD365
• HAVCR1
• HAVCR-1
• HAVCRT cell immunoglobin domain and mucin domain protein 1
• hepatitis A virus cellular receptor 1
• Kidney injury molecule 1
• KIM1
• KIM-1
• T-cell immunoglobulin and mucin domain-containing protein 1
• TIM1
• TIM-1
• TIM-1TIM
• TIM1TIMD-1
• TIMD1T-cell membrane protein 1

Background

TIM-1, also known as KIM-1 (Kidney-injury molecule-1) and HAVCR, is a 50-80 kDa, variably glycosylated, type I transmembrane glycoprotein that is a member of the TIM family of immunoglobulin superfamily molecules (1-5). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM/KIM genes (# 1-8) vs. only three genes in human (# 1, 3, 4) (1, 2, 5). It is unknown if the rat genome exactly parallels that of mouse. Rat TIM-1 is synthesized as a 307 amino acid (aa) precursor that contains a 21 aa signal sequence, a 214 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (4). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple Thr-Ser-Pro motifs. The mucin region may undergo extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and this may be reflected in rat (4, 6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing an 85 kDa soluble molecule (7). A similar process has now been described in rat (8). The ECD of rat TIM-1 is 50% and 81% aa identical to human and mouse TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (9, 10). However, others are believed to exist, and based on the ligand for TIM-3, one might be an S-type lectin (11). TIM-1 is found on CD4 + T cells and proximal renal tubular cells (4, 12). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 11). In particular, it induces IL-4 production, and requires the TIM-1 cytoplasmic tyrosine phosphorylation motif (12). Alternatively, TIM-1 activation of TIM-4 induces cell cycle arrest (13).

1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
4. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
5. Kuchroo, V.K. et al. (2006) Adv. Immunol. 91:227.
6. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
7. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
8. Vaidya, V.S. et al. (2006) Am. J. Physiol. Renal Physiol. 290:F517.
9. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
10. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
11. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
12. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
13. Mesri, M. et al. (2006) Int. Immunol. 18:473.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIM-1/KIM-1/HAVCR Antibody (MAB3689)(2)

Publications using MAB3689

• Y Nozaki, S Hino, J Ri, K Sakai, Y Nagare, M Kawanishi, K Niki, M Funauchi, I Matsumura Lipopolysaccharide-Induced Acute Kidney Injury Is Dependent on an IL-18 Receptor Signaling Pathway Int J Mol Sci, 2017-12-20;18(12):. 2017-12-20 [PMID: 29261164] (ICC, Mouse)
• FM Ornellas, DS Ornellas, SV Martini, RC Castiglion, GM Ventura, PR Rocco, B Gutfilen, SA de Souza, CM Takiya, MM Morales Bone Marrow-Derived Mononuclear Cell Therapy Accelerates Renal Ischemia-Reperfusion Injury Recovery by Modulating Inflammatory, Antioxidant and Apoptotic Related Molecules Cell. Physiol. Biochem., 2017-03-31;41(5):1736-1752. 2017-03-31 [PMID: 28365681] (IHC, Rat)

Bioinformatics

• Gene Symbol: HAVCR1
• Uniprot:
  • O54947()