TIM-1/KIM-1/HAVCR Antibody (511227) [Alexa Fluor® 488]

• Reactivity: Rt
• Applications: ELISA(Cap), ELISA(Det)
• Clone: 511227
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

TIM-1/KIM-1/HAVCR Antibody (511227) [Alexa Fluor® 488] Summary

• Immunogen: Mouse Myeloma cell line NS0-derived recombinant rat TIM-1/KIM-1/HAVCR
  Ser18-Val238
  Accession # O54947
• Specificity: Detects rat TIM-1/KIM-1/HAVCR in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse (rm) TIM-1, rmTIM-3, rmTIM-4, recombinant human (rh) TIM-1, rhTIM-4, or rhNKp44 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-1/KIM-1/HAVCR Antibody (511227) [Alexa Fluor® 488]

• CD365
• HAVCR1
• HAVCR-1
• HAVCRT cell immunoglobin domain and mucin domain protein 1
• hepatitis A virus cellular receptor 1
• Kidney injury molecule 1
• KIM1
• KIM-1
• T-cell immunoglobulin and mucin domain-containing protein 1
• TIM1
• TIM-1
• TIM-1TIM
• TIM1TIMD-1
• TIMD1T-cell membrane protein 1

Background

TIM-1, also known as KIM-1 (Kidney-injury molecule-1) and HAVCR, is a 50-80 kDa, variably glycosylated, type I transmembrane glycoprotein that is a member of the TIM family of immunoglobulin superfamily molecules (1-5). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM/KIM genes (# 1-8) vs. only three genes in human (# 1, 3, 4) (1, 2, 5). It is unknown if the rat genome exactly parallels that of mouse. Rat TIM-1 is synthesized as a 307 amino acid (aa) precursor that contains a 21 aa signal sequence, a 214 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (4). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple Thr-Ser-Pro motifs. The mucin region may undergo extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and this may be reflected in rat (4, 6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing an 85 kDa soluble molecule (7). A similar process has now been described in rat (8). The ECD of rat TIM-1 is 50% and 81% aa identical to human and mouse TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (9, 10). However, others are believed to exist, and based on the ligand for TIM-3, one might be an S-type lectin (11). TIM-1 is found on CD4 + T cells and proximal renal tubular cells (4, 12). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 11). In particular, it induces IL-4 production, and requires the TIM-1 cytoplasmic tyrosine phosphorylation motif (12). Alternatively, TIM-1 activation of TIM-4 induces cell cycle arrest (13).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O54947()