TIM-1/KIM-1/HAVCR Antibody (3B3)

Images

 
ELISA: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (NBP2-81119; red line) and ...read more
Surface Plasmon Resonance: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - Surface plasmon resonance (SPR) sensorgram TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119]. 3B3 binding to TIM-1 Fc-fusion protein. Binding of ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA, Flow, Func, ICC/IF, IHC, SPR
Clone
3B3
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TIM-1/KIM-1/HAVCR Antibody (3B3) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was raised by immunising female Lewis strain rats (Harlan Sprague-Dawley) subcutaneously with mouse TIM-1/KIM-1/HAVCR-Ig in complete Freund's adjuvant (CFA), followed by multiple 'boosting' with mouse TIM-1/KIM-1/HAVCR-Ig in PBS.
Specificity
This antibody is specific for murine TIM-1/KIM-1/HAVCR, a member of the T cell immunoglobulin domain, mucin-like domain (TIM) gene family. TIM-1/KIM-1/HAVCR has been found on activated CD4+ T cells, mast cells, and a subset of B cells. TIM-1/KIM-1/HAVCR is involved in renal injury, tissue homeostasis, and T cell costimulation, and promotes the differentiation of Th2 cells and the production of IL-4. TIM-1/KIM-1/HAVCR can bind several ligands, including Hepatitis A virus, phosphatidylserine, TIM4, IgA, as well as itself.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
HAVCR1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Surface Plasmon Resonance
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. This antibody has been used in multiple FACS analyses, such as to determine if specific B cell subsets or if B cell-derived interleukin-10 contributes to tolerance (Lai et al, 2015), to test if blocking several checkpoint receptors boosts anti-tumor immunity in a low-dose, lympho-depleting whole body irradiation model (Jing et al, 2015), and to study the functions of kidney pericytes in vascular stability (Schrimpf et al, 2012). This antibody has also been used in Western Blot and immunohistochemistry to investigate the distinct role of matrix metalloproteinase-3 in TIM-1 shedding by kidney proximal tubular epithelial cells (Lim et al. 2012), and to demonstrate how Bbeta(15-42) attenuates the effect of ischemia-reperfusion injury in renal transplantation (Sorensen et al, 2011). In addition, in vivo applications of this agonistic anti-TIM-1 antibody (clone 3B3) has been shown, for instance, to heighten T cell activation and prevent the development of respiratory tract tolerance in a Th2-driven model of asthma (Umetsu et al, 2005), to increase the frequency of antigen-specific T cells, the production of the proinflammatory cytokines IFN-gamma and IL-17, and thus the severity of experimental autoimmune encephalomyelitis (Sheng et al, 2007), as well as to deprogram Tregs and prevent transplant tolerance in mice (Degauque et al, 2008).

Packaging, Storage & Formulations

Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Buffer
PBS
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for TIM-1/KIM-1/HAVCR Antibody (3B3)

  • CD365
  • HAVCR1
  • HAVCR-1
  • HAVCRT cell immunoglobin domain and mucin domain protein 1
  • hepatitis A virus cellular receptor 1
  • Kidney injury molecule 1
  • KIM1
  • KIM-1
  • T-cell immunoglobulin and mucin domain-containing protein 1
  • TIM1
  • TIM-1
  • TIM-1TIM
  • TIM1TIMD-1
  • TIMD1T-cell membrane protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HAVCR1