TIM-1/KIM-1/HAVCR Antibody (3B3) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Mu
• Applications: ELISA, Flow, Func, ICC/IF, IHC, B/N, SPR
• Clone: 3B3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

TIM-1/KIM-1/HAVCR Antibody (3B3) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunising female Lewis strain rats (Harlan Sprague-Dawley) subcutaneously with mouse TIM-1/KIM-1/HAVCR-Ig in complete Freund's adjuvant (CFA), followed by multiple 'boosting' with mouse TIM-1/KIM-1/HAVCR-Ig in PBS.
• Specificity: This antibody is specific for murine TIM-1/KIM-1/HAVCR, a member of the T cell immunoglobulin domain, mucin-like domain (TIM) gene family. TIM-1/KIM-1/HAVCR has been found on activated CD4+ T cells, mast cells, and a subset of B cells. TIM-1/KIM-1/HAVCR is involved in renal injury, tissue homeostasis, and T cell costimulation, and promotes the differentiation of Th2 cells and the production of IL-4. TIM-1/KIM-1/HAVCR can bind several ligands, including Hepatitis A virus, phosphatidylserine, TIM4, IgA, as well as itself.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HAVCR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Surface Plasmon Resonance
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for TIM-1/KIM-1/HAVCR Antibody (3B3) [CoraFluor™ 1]

• CD365
• HAVCR1
• HAVCR-1
• HAVCRT cell immunoglobin domain and mucin domain protein 1
• hepatitis A virus cellular receptor 1
• Kidney injury molecule 1
• KIM1
• KIM-1
• T-cell immunoglobulin and mucin domain-containing protein 1
• TIM1
• TIM-1
• TIM-1TIM
• TIM1TIMD-1
• TIMD1T-cell membrane protein 1

Background

TIM 1 is a transmembrane protein containing immunoglobulin domain and mucin-like domain; it is developmentally expressed at very high levels in the blastocyst. TIM 1 binds hepatitis virus in humans and is expressed on activated CD4+ lymphocytes especially on Th2 cells and has been implicated to play a critical role in the development of atopic disease (in particular airway hyperreactivity) and other Th2-biased immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HAVCR1