TH1L Antibody - Azide and BSA Free

Western Blot: TH1L Antibody [NBP3-16475] - Western blot analysis of extracts of various cell lines, using TH1L antibody (NBP3-16475) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: TH1L Antibody [NBP3-16475] - Immunohistochemistry of paraffin-embedded rat testis using TH1L Rabbit pAb (NBP3-16475) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: TH1L Antibody [NBP3-16475] - Immunohistochemistry of paraffin-embedded human colon using TH1L Rabbit pAb (NBP3-16475) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: TH1L Antibody [NBP3-16475] - Immunohistochemistry of paraffin-embedded mouse spleen using TH1L Rabbit pAb (NBP3-16475) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TH1L Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-182 of human TH1L (NP_945327.3). HKFSTRDYIMEPSIFNTLKRYFQAGGSPENVIQLLSENYTAVAQTVNLLAEWLIQTGVEPVQVQETVENHLKSLLIKHFDPRKADSIFTEEGETPAWLEQMIAHTTWRDLFYKLAEAHPDCLMLNFTVKLISDAGYQGEITSV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NELFCD
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for TH1L Antibody - Azide and BSA Free

• HSPC130
• negative elongation factor proteins C and D
• NELF-C
• NELF-C/D
• NELFD
• NELF-D
• TH1 drosophila homolog
• TH1-like (Drosophila homolog)
• TH1-like (Drosophila)
• TH1-like protein
• TH1negative elongation factor C/D
• trihydrophobin 1

Background

The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NELFCD