TGF-beta 1 Antibody - BSA Free

Western Blot: TGF-beta 1 Antibody [NBP1-03276] - Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). The blot was incubated with a 1:200 dilution of the antibody at room temperature for 1 h followed by detection using labeled Goat-a-Rabbit IgG (H&L).

Immunohistochemistry-Paraffin: TGF-beta 1 Antibody [NBP1-03276] - Staining of human spleen tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

• Reactivity: Hu, Mu, Eq
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TGF-beta 1 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide from human TGF-beta 1.
• Specificity: A BLAST analysis was used to suggest reactivity with this protein from most mammalian sources based on 100% homology for the immunogen sequence. Cross-reactivity is expected with TGF-beta 1 from non-mammalian sources as only a single amino acid residue change is found within the immunogen sequence from many other organisms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGFB1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000 - 1:20000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:100 - 1:500
• Application Notes: IHC-Frozen reactivity reported in (PMID: 23611525). .

Reactivity Notes

Mouse reactivity reported in (PMID: 27713548), Equine reactivity reported in (PMID: 23611525).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for TGF-beta 1 Antibody - BSA Free

• CEDLAP
• DPD1
• latency-associated peptide
• TGF beta
• TGF beta1
• TGFB
• TGFB1
• TGF-beta 1 protein
• TGFbeta 1
• TGF-beta 1
• TGFbeta
• TGF-beta-1
• transforming growth factor beta-1
• transforming growth factor, beta 1

Background

TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, which includes three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25kDa homodimer composed of two 12.5kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule - the amino acid sequence between human and mouse differ only by one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues such as placenta and kidneys.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-beta 1 Antibody (NBP1-03276)(5)

Publications using NBP1-03276

• Li B, Chi X, Huang Y et Al. Bifidobacterium longum-Derived Extracellular Vesicles Prevent Hepatocellular Carcinoma by Modulating the TGF-?1/Smad Signaling in Mice Front Biosci (Landmark Ed) 2024-07-31 [PMID: 39082347]
• Gunin AG, Golubtzova NN Transforming Growth Factor-Beta (TGF-Beta) in Human Skin during Aging Advances in Gerontology 2018-11-14 [PMID: 31228363]
• Parween S, Kostromina E, Nord C et al. Intra-islet lesions and lobular variations in beta-cell mass expansion in ob/ob mice revealed by 3D imaging of intact pancreas. Sci Rep 2016-10-07 [PMID: 27713548] (IF/IHC, Mouse)
• Wang ZP, Mu XY, Guo M et al. Transforming growth factor-beta signaling participates in the maintenance of the primordial follicle pool in the mouse ovary. J. Biol. Chem. 2014-02-10 [PMID: 24515103] (WB, Mouse)
• Barsby T, Guest D. Transforming Growth Factor Beta3 Promotes Tendon Differentiation of Equine Embryo-derived Stem Cells. Tissue Eng Part A 2013-04-24 [PMID: 23611525] (WB, IHC-Fr, Equine)

Bioinformatics

• Gene Symbol: TGFB1
• Entrez:
  • 7040(Human)
• Uniprot:
  • P01137(Human)