TDP-43/TARDBP Antibody (S02-2A8)

Western Blot: TDP-43/TARDBP Antibody (S02-2A8) [NBP3-14980] - Western blot detection of K562, C6, 3T3, Hela cell lysates using NBP3-14980. Predicted band size: 45kDa. Observed band size: 45kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: S02-2A8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

TDP-43/TARDBP Antibody (S02-2A8) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human TDP-43/TARDBP (Uniprot#: Q13148)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TARDBP
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 0.0763888888888889
  • Western Blot 1:500~1:2000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TDP-43/TARDBP Antibody (S02-2A8)

• ALS10
• ALS10TAR DNA-binding protein-43
• TAR DNA binding protein
• TARDBP
• TDP43
• TDP-43
• TDP-43TAR DNA-binding protein 43

Background

TARDBP, also known as TAR DNA binding protein, is a DNA and RNA-binding protein which regulates transcription and splicing. TARDBP is also involved in the regulation of CFTR splicing and promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. TARD-BP may also be involved in microRNA biogenesis, apoptosis and cell division. TARDBP can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TARDBP