TAZ/WWTR1 Antibody

Images

 
Western Blot: TAZ/WWTR1 Antibody [NB110-58359] - WB analysis of Taz in HEK293T cell lysate. Verified customer review from 1DegreeBio.
Immunocytochemistry/ Immunofluorescence: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ (Green) in HepG2 cells using NB110-58359. Nuclei (Blue) are counterstained with Hoecsht 33258.
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NB110-58359] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using TAZ/WWTR1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The ...read more
Western Blot: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ on HEK 293 cell lysate using NB110-58359.
Simple Western: TAZ/WWTR1 Antibody [NB110-58359] - Simple Western lane view shows a specific band for TAZ in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TAZ/WWTR1 Antibody Summary

Immunogen
Synthetic peptides made to the human TAZ protein. These peptides were selected for the lack of homology to YAP protein. [UniProt# Q9GZV5]
Localization
Nuclear and cytoplasmic. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WWTR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Simple Western 1:100
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:100
Application Notes
This TAZ Antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot. Use in chromatin immunoprecipitation and Immunohistochemistry-paraffin reported in scientific literature (PMID 25587023).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
293 Lysate (NB800-PC6)
Kidney Lysate (NB820-59231)
TAZ/WWTR1 Lysate (NBL1-17895)
Reviewed Applications
Read 1 Review rated 3
using
NB110-58359 in the following applications:

Publications
Read Publications using
NB110-58359 in the following applications:

  • 2 publications
  • 1 publication
  • 1 publication
  • IP
    2 publications
  • WB
    5 publications

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TAZ/WWTR1 Antibody

  • FLJ27004
  • FLJ45718
  • TAZ
  • TAZDKFZp586I1419
  • transcriptional co-activator with PDZ-binding motif
  • Transcriptional coactivator with PDZ-binding motif
  • WW domain containing transcription regulator 1
  • WW domain-containing transcription regulator protein 1
  • WWTR1

Background

TAZ (transcriptional co-activator with PDZ-binding motif) was identified as a binding partner of 14-3-3 family of proteins which contains seven homologous proteins with ability to bind phosphorylated serine with certain sequence motifs, thereby involving in diverse cellular functions such as differentiation, cell cycle progression and apoptosis via their interaction with diverse intracellular phosphoproteins involved in signaling networks. TAZ is homologous to YAP and displays transcriptional co-activator function via interaction with PPXY-containing transcriptional factors through its WW domain. Several transcriptional factors such as Runx/PEBP2, AP2, C/EBP, c-Jun, Krox-20, Krox-24, MEF2B, NF-E2, Oct-4 and p73 containing proline-rich PPXY motif have been proposed as TAZ's interacting partners and TAZ is a major target of Hippo core kinase cascade which regulates organ size control as well as stem cell properties by governing cell proliferation and apoptosis processes Hippo mediated phosphorylation of YAP and TAZ leads to their sequestration into cytoplasm by interaction with 14-3-3 proteins and ubiquitination-dependent proteosomal degradation, thereby governing its distribution, protein levels and functionality also. TAZ interact primarily with transcriptional factors TEAD1-4 (TEADs) and activate expression of target genes such as CTGF, IGFBP3, ITGB2, Birc5/Survivin, Gli2, and Axl.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TAZ/WWTR1 Antibody (NB110-58359)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ChIP, ICC/IF, IHC-P, IP, WB.


Filter By Application
ChIP
(2)
ICC/IF
(1)
IHC-P
(1)
IP
(2)
WB
(5)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Rat
(1)
All Species

Review for TAZ/WWTR1 Antibody (NB110-58359) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB110-58359:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot TAZ/WWTR1 NB110-58359
Enlarge
reviewed by:
Andrew Cosgrove
WB Human 11/06/2013
View

Summary

ApplicationWestern Blot
Sample TestedHEK293T cell lysate
SpeciesHuman
CommentsDetects human TAZ (WWTR1) and not YAP.

Primary Anitbody

Dilution Ratio1:1000 in 1%BSA in TBST

Comments

CommentsDetects human TAZ (WWTR1) and not YAP.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for TAZ/WWTR1 Antibody (NB110-58359). (Showing 1 - 3 of 3 FAQs).

  1. I want to know which TAZ primary antibody is best produced for WB (human and mouse)!
    • I would recommend this TAZ antibody: NB110-58359. This is also comes in a sample size.
  2. We have an inquiry for your product #NB110-58359, TAZ Antibody. Our customer would like to try this product with rat cells lysate. Could you please let me know if you have data image of rat cells lysate can provide?
    • This antibody has been validated and is therefore guaranteed for use in human, rat and mouse in the following applications: Western blot, Immunoprecipitation and ICC/IF. We do not currently have a WB image that we can share however rat is one of the guaranteed species.
  3. Our customer was interested in the TAZ Antibody (Cat.NB110-58359). Could you please help to confirm if it has been tested with rat cells?
    • The TAZ antibody, NB110-58359, has been tested and validated for use in rat and is therefore guaranteed. Our 100% guarantee covers all of our products for the listed species and applications.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TAZ/WWTR1 Antibody (NB110-58359)

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Research Areas for TAZ/WWTR1 Antibody (NB110-58359)

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Blogs on TAZ/WWTR1

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Recent Reviews

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Andrew Cosgrove
11/06/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol WWTR1
Entrez
Uniprot