TAZ/WWTR1 Antibody

Images

 
Western Blot: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ on HEK 293 cell lysate using NB110-58359.
Immunocytochemistry/ Immunofluorescence: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ (Green) in HepG2 cells using NB110-58359. Nuclei (Blue) are counterstained with Hoecsht 33258.
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NB110-58359] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using TAZ/WWTR1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The ...read more
Simple Western: TAZ/WWTR1 Antibody [NB110-58359] - Simple Western lane view shows a specific band for TAZ in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TAZ/WWTR1 Antibody Summary

Immunogen
Synthetic peptides made to the human TAZ protein. These peptides were selected for the lack of homology to YAP protein. [UniProt# Q9GZV5]
Localization
Nuclear and cytoplasmic. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WWTR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Simple Western 1:100
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:100
  • Proximity Ligation Assay
Application Notes
This TAZ Antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot. Use in chromatin immunoprecipitation and Immunohistochemistry-paraffin reported in scientific literature (PMID 25587023). Use in Proximity Ligation Assay reported in scientific literature (PMID: 25587023).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
293 Whole Cell Lysate
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
TAZ/WWTR1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 3
using
NB110-58359 in the following applications:

Publications
Read Publications using
NB110-58359 in the following applications:

  • 3 publications
  • 2 publications
  • 2 publications
  • IP
    3 publications
  • WB
    7 publications

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TAZ/WWTR1 Antibody

  • FLJ27004
  • FLJ45718
  • TAZ
  • TAZDKFZp586I1419
  • transcriptional co-activator with PDZ-binding motif
  • Transcriptional coactivator with PDZ-binding motif
  • WW domain containing transcription regulator 1
  • WW domain-containing transcription regulator protein 1
  • WWTR1

Background

TAZ (transcriptional co-activator with PDZ-binding motif) was identified as a binding partner of 14-3-3 family of proteins which contains seven homologous proteins with ability to bind phosphorylated serine with certain sequence motifs, thereby involving in diverse cellular functions such as differentiation, cell cycle progression and apoptosis via their interaction with diverse intracellular phosphoproteins involved in signaling networks. TAZ is homologous to YAP and displays transcriptional co-activator function via interaction with PPXY-containing transcriptional factors through its WW domain. Several transcriptional factors such as Runx/PEBP2, AP2, C/EBP, c-Jun, Krox-20, Krox-24, MEF2B, NF-E2, Oct-4 and p73 containing proline-rich PPXY motif have been proposed as TAZ's interacting partners. TAZ is also a major target of the Hippo core kinase cascade which regulates organ size control as well as stem cell properties by governing cell proliferation and apoptosis processes. Hippo mediated phosphorylation of YAP and TAZ leads to their sequestration into the cytoplasm by interaction with 14-3-3 proteins and ubiquitination-dependent proteosomal degradation, thereby governing its distribution, protein levels and functionality. TAZ interacts primarily with transcriptional factors TEAD1-4 (TEADs) and activates expression of target genes such as CTGF, IGFBP3, ITGB2, Birc5/Survivin, Gli2, and Axl.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TAZ/WWTR1 Antibody (NB110-58359)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ChIP, ICC/IF, IHC-P, IP, WB.


Filter By Application
ChIP
(3)
ICC/IF
(2)
IHC-P
(2)
IP
(3)
WB
(7)
All Applications
Filter By Species
Human
(4)
Mouse
(4)
Rat
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB110-58359 Applications Species
Li W, Zhao J, Wang J et al. ROCK-TAZ signaling axis regulates mechanical tension-induced osteogenic differentiation of rat cranial sagittal suture mesenchymal stem cells J. Cell. Physiol. Jan 22 2020 [PMID: 31970784] (IP, WB, Rat) IP, WB Rat
Kegelman CD, Coulombe JC, Jordan KM et al. YAP and TAZ Mediate Osteocyte Perilacunar/Canalicular Remodeling J. Bone Miner. Res. 2019 Oct 14 [PMID: 31610061] (Mouse) Mouse
Hwang JH, Kim AR, Kim KM et al. TAZ couples Hippo/Wnt signalling and insulin sensitivity through Irs1 expression. Nat Commun Jan 24 2019 [PMID: 30679431] (ChIP, Mouse) ChIP Mouse
Yu B, Huo L, Liu Y et al. PGC-1a Controls Skeletal Stem Cell Fate and Bone-Fat Balance in Osteoporosis and Skeletal Aging by Inducing TAZ. Cell Stem Cell. Jul 3 2018 [PMID: 30017591] (IHC-P, ICC/IF, WB, Mouse) IHC-P, ICC/IF, WB Mouse
Wu LMN, Deng Y, Wang J, Zhao C. Programming of Schwann Cells by Lats1/2-TAZ/YAP Signaling Drives Malignant Peripheral Nerve Sheath Tumorigenesis. Cancer Cell. 2018 Feb 12 [PMID: 29438698] (Human) Human
Deng Y, Wu LMN, Bai S et al. A reciprocal regulatory loop between TAZ/YAP and G-protein Gas regulates Schwann cell proliferation and myelination. Nat Commun Apr 26 2017 [PMID: 28443644] (WB, Human) WB Human
Xiang L, Gilkes DM, Hu H et al. HIF-1alpha and TAZ serve as reciprocal co-activators in human breast cancer cells. Oncotarget. 2015 May 20 [PMID: 26059435] (IP, ChIP, WB, Human) IP, ChIP, WB Human
Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014 [PMID: 25587023] (ChIP, IP, WB, IHC-P, ICC/IF, Human) ChIP, IP, WB, IHC-P, ICC/IF Human
de Cristofaro T, Di Palma T, Ferraro A et al. TAZ/WWTR1 is overexpressed in papillary thyroid carcinoma Eur J Cancer 2011 Apr [PMID: 21131195] (WB, Rat) WB Rat
Varelas X, Miller BW, Sopko R et al. The Hippo pathway regulates Wnt/beta-catenin signaling. Dev Cell;18(4):579-91. 2010 Apr 20. [PMID: 20412773] (WB, Mouse) WB Mouse
Show All 11 Publications.

Review for TAZ/WWTR1 Antibody (NB110-58359) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-58359:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot TAZ/WWTR1 NB110-58359
Enlarge
reviewed by:
Andrew Cosgrove
WB Human 11/06/2013
View

Summary

ApplicationWestern Blot
Sample TestedHEK293T cell lysate
SpeciesHuman
CommentsDetects human TAZ (WWTR1) and not YAP.

Primary Anitbody

Dilution Ratio1:1000 in 1%BSA in TBST

Comments

CommentsDetects human TAZ (WWTR1) and not YAP.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for TAZ/WWTR1 Antibody (NB110-58359). (Showing 1 - 4 of 4 FAQs).

  1. I want to know which TAZ primary antibody is best produced for WB (human and mouse)!
    • I would recommend this TAZ antibody: NB110-58359. This is also comes in a sample size.
  2. We have an inquiry for your product #NB110-58359, TAZ Antibody. Our customer would like to try this product with rat cells lysate. Could you please let me know if you have data image of rat cells lysate can provide?
    • This antibody has been validated and is therefore guaranteed for use in human, rat and mouse in the following applications: Western blot, Immunoprecipitation and ICC/IF. We do not currently have a WB image that we can share however rat is one of the guaranteed species.
  3. Our customer was interested in the TAZ Antibody (Cat.NB110-58359). Could you please help to confirm if it has been tested with rat cells?
    • The TAZ antibody, NB110-58359, has been tested and validated for use in rat and is therefore guaranteed. Our 100% guarantee covers all of our products for the listed species and applications.
  4. I am looking for a WWTR1 antibody and NB110-58359 looks like the best one. I wonder if you guys can provide me the target sequence information of the antibody?
    • WWTR1 antibody (NB110-58359) is generated against residues 178-197 of WWTR1 (immunogen accession number Q9GZV5). The exact immunogen sequence for this antibody is: CPQRSMAVSQPNLVMNHQHQQ

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-58359AF350
Alexa Fluor 405 NB110-58359AF405
Alexa Fluor 488 NB110-58359AF488
Alexa Fluor 532 NB110-58359AF532
Alexa Fluor 594 NB110-58359AF594
Alexa Fluor 647 NB110-58359AF647
Alexa Fluor 700 NB110-58359AF700
Alexa Fluor 750 NB110-58359AF750
Biotin NB110-58359B
DyLight 350 NB110-58359UV
DyLight 405 NB110-58359V
DyLight 488 NB110-58359G
DyLight 550 NB110-58359R
DyLight 594 NB110-58359DL594
DyLight 650 NB110-58359C
DyLight 680 NB110-58359FR
DyLight 755 NB110-58359IR
FITC NB110-58359F
HRP NB110-58359H
Janelia Fluor 549 NB110-58359JF549
Janelia Fluor 646 NB110-58359JF646

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Bioinformatics Tool for TAZ/WWTR1 Antibody (NB110-58359)

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Blogs on TAZ/WWTR1

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Contact Information

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Recent Reviews

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Andrew Cosgrove
11/06/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol WWTR1
Entrez
Uniprot