| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | DyLight 594 |
| Immunogen | Synthetic peptides made to the human TAZ protein. These peptides were selected for the lack of homology to YAP protein. [UniProt# Q9GZV5] |
| Localization | Nuclear and cytoplasmic. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | WWTR1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NB110-58359AF350 | |
| Alexa Fluor 405 | NB110-58359AF405 | |
| Alexa Fluor 488 | NB110-58359AF488 | |
| Alexa Fluor 532 | NB110-58359AF532 | |
| Alexa Fluor 594 | NB110-58359AF594 | |
| Alexa Fluor 647 | NB110-58359AF647 | |
| Alexa Fluor 700 | NB110-58359AF700 | |
| Alexa Fluor 750 | NB110-58359AF750 | |
| Biotin | NB110-58359B | |
| DyLight 350 | NB110-58359UV | |
| DyLight 405 | NB110-58359V | |
| DyLight 488 | NB110-58359G | |
| DyLight 550 | NB110-58359R | |
| DyLight 594 | NB110-58359DL594 | |
| DyLight 650 | NB110-58359C | |
| DyLight 680 | NB110-58359FR | |
| DyLight 755 | NB110-58359IR | |
| FITC | NB110-58359F | |
| HRP | NB110-58359H | |
| Janelia Fluor 549 | NB110-58359JF549 | |
| Janelia Fluor 646 | NB110-58359JF646 |
Diseases for TAZ/WWTR1 Antibody (NB110-58359DL594)Discover more about diseases related to TAZ/WWTR1 Antibody (NB110-58359DL594).
| Pathways for TAZ/WWTR1 Antibody (NB110-58359DL594)View related products by pathway.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | WWTR1 |
![Knockout Validated: YAP1 Antibody [NB110-58358] - Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A specific band was detected for YAP1 at approximately 81 kDa (as indicated) using 50 ug/mL of Rabbit Anti-YAP1 Polyclonal Antibody (Catalog # NB110-58358). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/images2/YAP1-Antibody-Knockout-Validated-NB110-58358-img0009.jpg)
![Knockout Validated: YAP1 Antibody [NB110-58358] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human YAP1 Polyclonal Antibody (Catalog # NB110-58358) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/YAP1-Antibody-Knockout-Validated-NB110-58358-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: YAP1 Antibody [NB110-58358] - Analysis in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (red) and DAPI (blue), respectively.](http://images.novusbio.com/images2/YAP1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58358-img0006.jpg)
![Immunohistochemistry-Paraffin: YY1AP1 Antibody [NBP1-81763] Staining of human kidney shows strong cytoplasmic and membranous positivity in tubules.](http://images.novusbio.com/images/YY1AP1 Antibody-Immunohistochemistry-Paraffin-NBP1-81763-img0003.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-77461] - Vascularization of mineralized cell-laden collagen and interaction with prostate cancer cells. HUVECs formed endothelial networks that were supported by SMA-expressing hMSCs (scale bar: 50m) and were also positive for CD31 (scale bar: 400m). The remainder of hMSCs expressed RUNX2 as a marker for osteogenic differentiation (scale bar: 50m). Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralization. Nat Commun (2019)](http://images.novusbio.com/images2/RUNX2-CBFA1 Antibody-Immunohistochemistry-Paraffin-NBP1-77461-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-77461] - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/RUNX2-CBFA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77461-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-77461] - Characterization of TLC cultured in chondrogenic pellets. Gene expression of TLC cultured four weeks in chondrogenic pellets or in cell monolayer in control medium: levels of expression of osteoblast markers (1), chondrocyte markers (2) and tenocyte markers (3) (N = 3) assessed by qPCR.* =0.05. Rotator Cuff Tenocytes Differentiate into Hypertrophic Chondrocyte-Like Cells to Produce Calcium Deposits in an Alkaline Phosphatase-Dependent Manner. J Clin Med (2019)](http://images.novusbio.com/images2/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-77461-img0008.jpg)
![Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.](http://images.novusbio.com/images2/PPAR-gamma-NR1C3-Antibody-Western-Blot-NBP2-22106-img0003.jpg)
![Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.](http://images.novusbio.com/images2/PPAR-gamma-Antibody-Immunohistochemistry-Paraffin-NBP2-22106-img0001.jpg)
![Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.](http://images.novusbio.com/images2/PPAR-gamma-Antibody-Western-Blot-NBP2-22106-img0002.jpg)
![Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Lung Carcinoma stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)](http://images.novusbio.com/images2/TTF-1---NKX2-1-Antibody-(8G7G3-1-+-NX2.1-690)-Immunohistochemistry-Paraffin-NBP2-44501-img0001.jpg)
![Immunohistochemistry: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - TTF1 staining in thyroid tissue of wild-type C57 mouse (red). Dilution is 1:100. Image from a verified customer review.](http://images.novusbio.com/images2/TTF-1---NKX2-1-Antibody-(8G7G3-1-+-NX2.1-690)-Immunohistochemistry-NBP2-44501-img0003.jpg)
![Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Thyroid stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)](http://images.novusbio.com/images2/TTF-1---NKX2-1-Antibody-(8G7G3-1-+-NX2.1-690)-Immunohistochemistry-Paraffin-NBP2-44501-img0002.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Western-Blot-NB300-560-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Paraffin-NB300-560-img0010.jpg)
![Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Frozen-NB300-560-img0009.jpg)
![Western Blot: LATS1 Antibody [NBP1-86860] - Analysis in human cell line HELA.](http://images.novusbio.com/images2/LATS1-Antibody-Western-Blot-NBP1-86860-img0005.jpg)
![Immunohistochemistry-Paraffin: LATS1 Antibody [NBP1-86860] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/images2/LATS1 Antibody-Immunohistochemistry-Paraffin-NBP1-86860-img0007.jpg)
![Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/images2/14-3-3 Antibody-Western Blot-NBP2-27202-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 Antibody [NBP2-27202] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-14-3-3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/14-3-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27202-img0004.jpg)
![Simple Western: 14-3-3 Antibody [NBP2-27202] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/14-3-3-Antibody-Simple-Western-NBP2-27202-img0002.jpg)
![Western Blot: AMOT Antibody (OTI1A8) - Azide and BSA Free [NBP2-72429] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMOT.](http://images.novusbio.com/images2/AMOT-Antibody-(OTI1A8)---Azide-and-BSA-Free-Western-Blot-NBP2-72429-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: AMOT Antibody (OTI1A8) - Azide and BSA Free [NBP2-72429] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY AMOT.](http://images.novusbio.com/images2/AMOT Antibody (OTI1A8) - Azide and BSA Free-Immunocytochemistry-Immunofluorescence-NBP2-72429-img0002.jpg)
![Western Blot: Musculin Antibody [NBP2-56244] - Western blot analysis in human cell line RT-4.](http://images.novusbio.com/images2/Musculin Antibody-Western Blot-NBP2-56244-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Musculin Antibody [NBP2-56244] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm.](http://images.novusbio.com/images2/Musculin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56244-img0001.jpg)
![Immunohistochemistry-Paraffin: Prosurfactant Protein C Antibody [NBP1-87201] - Analysis in human lung and kidney tissues using HPA010928 antibody. Corresponding SFTPC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Prosurfactant-Protein-C-Antibody-Immunohistochemistry-Paraffin-NBP1-87201-img0008.jpg)
![Western Blot: Prosurfactant Protein C Antibody [NBP1-87201] - Analysis in human lung tissue.](http://images.novusbio.com/images2/Prosurfactant-Protein-C-Antibody-Western-Blot-NBP1-87201-img0005.jpg)
![Immunohistochemistry-Paraffin: Prosurfactant Protein C Antibody [NBP1-87201] - Staining of human kidney shows no positivity in cells in glomeruli as expected.](http://images.novusbio.com/images2/Prosurfactant-Protein-C-Antibody-Immunohistochemistry-Paraffin-NBP1-87201-img0006.jpg)
![Western Blot: LDB3 Antibody (3C8) [H00011155-M06] - Analysis of LDB3 expression in A-431 (Cat # L015V1).](http://images.novusbio.com/images2/LDB3-Antibody-(3C8)-Western-Blot-H00011155-M06-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: LDB3 Antibody (3C8) [H00011155-M06] - Analysis of monoclonal antibody to LDB3 on A-431 cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/images2/LDB3-Antibody-(3C8)-Immunocytochemistry-Immunofluorescence-H00011155-M06-img0007.jpg)
![Western Blot: LDB3 Antibody (3C8) [H00011155-M06] - Western blot analysis of LDB3 over-expressed 293 cell line, cotransfected with LDB3 Validated Chimera RNAi or non-transfected control. Blot probed with H00011155-M06. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/images2/LDB3-Antibody-(3C8)-Western-Blot-H00011155-M06-img0004.jpg)
![Western Blot: ASRGL1 Antibody [NBP1-89133] - Analysis in control (vector only transfected HEK293T lysate) and ASRGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/ASRGL1-Antibody-Western-Blot-NBP1-89133-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: ASRGL1 Antibody [NBP1-89133] - Staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules. Antibody staining is shown in green.](http://images.novusbio.com/images2/ASRGL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89133-img0004.jpg)
![Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133] - Staining of human cervix, uterine, endometrium, fallopian tube and skeletal muscle using Anti-ASRGL1 antibody NBP1-89133 (A) shows similar protein distribution across tissues to independent antibody NBP2-38759 (B).](http://images.novusbio.com/images2/ASRGL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89133-img0012.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")