GFER/ALR Antibody (829812)

HAF018). Specific bands were detected for GFER/ALR at approximately 20-24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF018). Specific bands were detected for GFER/ALR at approximately 20-24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GFER/ALR Monoclonal Antibody (Catalog # MAB7940) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GFER/ALR at approximately 20-24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GFER/ALR Monoclonal Antibody (Catalog # MAB7940) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="GFER/ALR was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human GFER/ALR Monoclonal Antibody (Catalog # MAB7940) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

GFER/ALR was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human GFER/ALR Monoclonal Antibody (Catalog # MAB7940) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 829812
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GFER/ALR Antibody (829812) Summary

• Immunogen: E. coli-derived recombinant human GFER/ALR
  Arg82-Asp205
  Accession # P55789
• Specificity: Detects human GFER/ALR in ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GFER
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFER/ALR Antibody (829812)

• ALR
• ALRFAD-linked sulfhydryl oxidase ALR
• Augmenter of liver regeneration
• EC 1.8.3.2
• ERV1 homolog
• ERV1
• ERV1hepatopoietin protein
• erv1-like growth factor
• GFER
• growth factor, augmenter of liver regeneration
• growth factor, erv1 (S. cerevisiae)-like (augmenter of liver regeneration)
• Hepatopoietin
• HERV1
• HERV1hepatic regenerative stimulation substance
• HPO
• HPO1
• HPO2
• HSS
• truncated augmenter of liver regeneration

Background

GFER, also known as Augmenter of Liver Regeneration (ALR) and Hepatopoietin, is an approximately 30 kDa mitochondrial sulfhydryl oxidase that plays a role in protein disulfide bond formation. Alternative splicing of human GFER generates a 15 kDa secreted isoform that lacks the 80 N-terminal amino acids. Within aa 82‑205, human GFER shares approximately 86% aa sequence identity with mouse and rat GFER. GFER protects hepatocytes from toxin-induced damage, promotes hepatic regeneration, and inhibits the metastasis of hepatocellular carcinoma. It also restricts the proliferation of pluripotent and hematopoietic stem cells and maintains mitochondrial integrity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFER/ALR Antibody (MAB7940)(1)

Publication using MAB7940

• C Sima, E Montero, D Nguyen, M Freire, P Norris, CN Serhan, TE Van Dyke ERV1 Overexpression in Myeloid Cells Protects against High Fat Diet Induced Obesity and Glucose Intolerance Sci Rep, 2017-10-09;7(1):12848. 2017-10-09 [PMID: 28993702] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: GFER
• Uniprot:
  • P55789()