YAP1 Antibody


Genetic Strategies: Knockout Validated: YAP1 Antibody [NB110-58358] - Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A ...read more
Genetic Strategies: Knockout Validated: YAP1 Antibody [NB110-58358] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane ...read more
Immunocytochemistry/ Immunofluorescence: YAP1 Antibody [NB110-58358] - Analysis in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (red) and ...read more
Immunohistochemistry-Paraffin: YAP1 Antibody [NB110-58358] - YAP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human YAP1 polyclonal Antibody (Catalog # NB110-58358) at ...read more
Western Blot: YAP1 Antibody [NB110-58358] - Actomyosin activity inhibits beta-catenin- and YAP-driven proliferation of confluent keratinocytes. Effects of cell density and actomyosin activity on YAP phosphorylation. ...read more
Western Blot: YAP1 Antibody [NB110-58358] - Analysis in transfected HEK 293 cell lysate using YAP1 antibody. Observed molecular weight 75 kDa.
Western Blot: YAP1 Antibody [NB110-58358] - Consequences of ORP5 and ORP8 knockdown on downstream MAPK and PI3K/AKT signaling. Protein from MOH parental, single and double ORP knockdowns as well as cells transfected ...read more
Simple Western: YAP1 Antibody [NB110-58358] - Simple Western lane view shows a specific band for YAP1 in 0.1 mg/ml of HeLa lysate. Observed molecular weight is 75 kDa. This experiment was performed under reducing ...read more

Product Details

Reactivity Hu, Mu, Rt, Ca, Dr, ZeSpecies Glossary
Applications WB, Simple Western, ChIP, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO
1.0 mg/ml
Validated by:

Genetic Strategies


YAP1 Antibody Summary

This YAP1 Antibody was developed against a partial recombinant human YAP1 protein expressed in bacteria. [Uniprot: P46937], N-terminal GST fusion protein
Expected reactivity based on immunogen homology: Isoform 4 (100%), Isoform 6 (100%)
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 2-10 ug
  • Knockdown Validated
  • Knockout Validated
  • Simple Western 1:12.5
  • Western Blot 1:1000
Application Notes
Use in Immunoblotting reported in scientific literature (PMID: 28406163). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 28581498). Use in Knockdown Validated reported in scientific literature (PMID:28406163). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
293 Whole Cell Lysate
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 2 Reviews rated 3.5
NB110-58358 in the following applications:

Read Publications using
NB110-58358 in the following applications:

Reactivity Notes

Use in Human reported in scientific literature (PMID:33737385). Use in Zebrafish reported in scientific literature (PMID:28350986).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for YAP1 Antibody

  • 65 kDa Yes-associated protein
  • COB1
  • Protein Yorkie Homolog
  • Transcriptional Coactivator YAP1
  • YAP
  • YAP1
  • YAP1-2gamma
  • YAP2
  • YAP2L
  • YAP65
  • YAP65yes-associated protein 2
  • Yes Associated Protein
  • Yes-associated protein 1
  • Yes-associated protein 1, 65kDa
  • yes-associated protein beta
  • yes-associated protein delta
  • Yes-Associated Protein YAP65 Homolog
  • Yes-Associated Protein
  • YKI
  • Yorkie Homolog


The transcriptional coactivator Yes-associated protein (YAP), also known as Yes-associated protein 1 (YAP1), Yes-Associated Protein YAP65 Homolog, Protein Yorkie Homolog and YAP65 has a theoretical molecular weight of 65 kDa. The YAP1 gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. YAP1 shares homology with the WW domain of transcriptional co-activator with PDZ-binding motif (TAZ), which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors and is likely involved in protein-protein interaction. YAP has been identified as a core regulatory mechanism that blocks mammalian glial cell proliferation and cellular reprogramming following damage (1).

YAP plays a role in the development and progression of multiple cancers as a transcriptional regulator of the Hippo signaling pathway. YAP1 encodes a nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis to play a pivotal role in controlling cell growth and organ size and has emerged as a key player in tumor suppression (2,3). Deregulation of the Hippo pathway causes tumor formation and malignancy, with YAP being a key oncogenic driver in liver carcinogenesis (2) and may function as a potential target for cancer treatment (3).


1. Rueda, E. M., Hall, B. M., Hill, M. C., Swinton, P. G., Tong, X., Martin, J. F., & Poche, R. A. (2019). The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming. Cell Rep, 27(6), 1637-1649.e1636. doi:10.1016/j.celrep.2019.04.047

2. Liu, A. M., Xu, M. Z., Chen, J., Poon, R. T., & Luk, J. M. (2010). Targeting YAP and Hippo signaling pathway in liver cancer. Expert Opin Ther Targets, 14(8), 855-868. doi:10.1517/14728222.2010.499361

3.Ye, S., & Eisinger-Mathason, T. S. (2016). Targeting the Hippo pathway: Clinical implications and therapeutics. Pharmacol Res, 103, 270-278. doi:10.1016/j.phrs.2015.11.025


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for YAP1 Antibody (NB110-58358)(57)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Zebrafish.

We have publications tested in 9 applications: ChIP, ChIP-seq, ICC/IF, IHC, IHC-F, IHC-P, IP, KD, WB.

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Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using NB110-58358 Applications Species
Wang EY. Transcriptional regulation by the Hippo signaling pathway in the liver. Thesis. Jul 9 1905 (ChIP, Mouse) ChIP Mouse
He Q, Gao Y, Zhou L et al. Deficiency of Yes-Associated Protein Induces Cataract in Mice Aging and disease Jul 9 1905 (Mouse) Mouse
Zhu M, Peng R, Liang X, et al. P4HA2-induced prolyl hydroxylation suppresses YAP1-mediated prostate cancer cell migration, invasion, and metastasis bioRxiv Jan 26 2021 (ChIP-seq, Mouse) ChIP-seq Mouse
Savorani C, Malinverno M, Seccia R Et al. A dual role of YAP in driving TGF beta-mediated endothelial-to-mesenchymal transition Journal of cell science Aug 1 2021 [PMID: 34338295]
Aharonov A, Shakked A, Umansky KB, Savidor A ERBB2 drives YAP activation and EMT-like processes during cardiac regeneration bioRxiv Jan 1 2020 (IP, Mouse) IP Mouse
Hicks-Berthet J, Ning B, Federico A Et Al. Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis Cell reports Jul 13 2021 [PMID: 34260916] (ChIP) ChIP
Chakravarti D, Lee R, Multani As Et Al. Telomere dysfunction instigates inflammation in inflammatory bowel disease Proceedings of the National Academy of Sciences of the United States of America Jul 20 2021 [PMID: 34253611] (IHC, WB) IHC, WB
Liu S, Li K, Wagner Florencio L Et Al. Gene therapy knockdown of Hippo signaling induces cardiomyocyte renewal in pigs after myocardial infarction Science translational medicine Jun 30 2021 [PMID: 34193613]
Tang Q, Efe G, Chiarella AM et al. Mutant p53 regulates Survivin to foster lung metastasis Genes & development Mar 18 2021 [PMID: 33737385] (ChIP, Human) ChIP Human
Li C, Peng Z, Wang Y, et al. Epithelial Cell Transforming 2 (ECT2) is regulated by Yes-associated protein 1 (YAP1) and mediates pancreatic cancer progression and metastasis American journal of physiology. Gastrointestinal and liver physiology Jan 27 2021 [PMID: 33501895]
Show All 57 Publications.

Reviews for YAP1 Antibody (NB110-58358) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Zebrafish

Reviews using NB110-58358:
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Images Ratings Applications Species Date Details
Western Blot YAP1 NB110-58358
reviewed by:
Verified Customer
WB Human 02/04/2019


ApplicationWestern Blot
Sample TestedHuman prostate cancer cell line (PC3)
Western Blot YAP1 NB110-58358
reviewed by:
Verified Customer
WB Zebrafish


ApplicationWestern Blot
Sample TestedProtein Lysate


CommentsLysate prepared with RIPA buffer. Reducing agent was used. 70 deg C for 10 min. Ran on 4-12% bis tris gel at 200v for 40 min.Primary antibody was used at a dilution of 1:1000 and incubated overnight at 4C. Secondary antibody was RDyde 800 CW Goat anti-Rabbit IgG diluted 1:5000 incubated overnight at 4C. Detection method was Licor Odyssey detection.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for YAP1 Antibody (NB110-58358). (Showing 1 - 2 of 2 FAQs).

  1. I am trying to find a good antibody for YAP1 to use in a ChIP. The one that has been published and seems to work in ChIP was produced by Marius Sudol's lab. Is the YAP antibody NB110-58358 the one Marius Sudol sent you or did you produce it using his antigen?
    • You are correct. This antibody is from Sudol's lab, and yes it was produced directly by him.
  2. Our customer purchased NB110-58358 for IHC-P. The sample would be mouse skin. Now they face the problem of choosing which protocol of antigen retrieval is the best one to work with NB110-58358, since there are several listed on http://www.novusbio.com/support/support-by-application/antigen-retrieval/protocol.html. Would you please help suggest?
    • To answer your customer's question, we do not have any protocol specific to NB110-58358 but as a general guideline we usually recommend using Citrate buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0) for antigen retrieving. 20 minutes incubation is usually sufficient in our experience.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-58358AF350
Alexa Fluor 532 NB110-58358AF532
Alexa Fluor 594 NB110-58358AF594
Alexa Fluor 750 NB110-58358AF750
DyLight 350 NB110-58358UV
DyLight 594 NB110-58358DL594
DyLight 680 NB110-58358FR
DyLight 755 NB110-58358IR
Janelia Fluor 549 NB110-58358JF549
Janelia Fluor 646 NB110-58358JF646

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Blogs on YAP1.

  Read full blog post.

YAP1 - a transcription co-activator and the downstream target of Hippo pathway
YAP1 (Yes-associated protein 1) is a  transcriptional co-activator which acts as a major effector of Hippo signaling pathway that regulates organ size/ tissue homeostasis and cell proliferation, and is an established oncogene (1).  Hippo sig...  Read full blog post.

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Recent Reviews


Verified Customer
Application: WB
Species: Human

Verified Customer
Application: WB
Species: Zebrafish


Gene Symbol YAP1