YAP1 Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: YAP1 Antibody - BSA Free [NB110-58358] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Biological Strategies: Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - LW6 attenuates the accumulation of cellular YAP1 and its nuclear location. After treating PANC-1 cells with LW6 for 12 hours, LW6 ...read more
Immunocytochemistry/ Immunofluorescence: YAP1 Antibody - BSA Free [NB110-58358] - YAP/TAZ signaling is not activated by loss of TRAF3. NT, NF2 KO1, and TRAF3 KO1 cells stained for YAP1 and DAPI. Image collected and ...read more
Immunohistochemistry-Paraffin: YAP1 Antibody - BSA Free [NB110-58358] - YAP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human YAP1 polyclonal Antibody (Catalog # ...read more
Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - Analysis in transfected HEK 293 cell lysate using YAP1 antibody. Observed molecular weight 75 kDa.
Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - Consequences of ORP5 and ORP8 knockdown on downstream MAPK and PI3K/AKT signaling. Protein from MOH parental, single and double ORP knockdowns as well as cells ...read more
Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - YAP1 Antibody [NB110-58358] - Actomyosin activity inhibits beta-catenin- and YAP-driven proliferation of confluent keratinocytes. Effects of cell density and ...read more
Simple Western: YAP1 Antibody - BSA Free [NB110-58358] - Simple Western lane view shows a specific band for YAP1 in 0.1 mg/ml of HeLa lysate. Observed molecular weight is 75 kDa. This experiment was performed under ...read more
Knockout Validated: YAP1 Antibody - BSA Free [NB110-58358] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with ...read more
Knockout Validated: YAP1 Antibody - BSA Free [NB110-58358] - Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A specific band was ...read more
Actomyosin activity inhibits beta-catenin- and YAP-driven proliferation of confluent keratinocytes. (e and f) Confluent HaCaT cells cultured for 40 h were treated for 6 h with 100 uM blebbistatin (Blebb) or DMSO (for ...read more
YAP and TAZ expression at high density and during chondrogenic differentiation of human synovial MSCs. (A) YAP and pYAP expression in human synovial membrane-derived (hSM-)MSCs in monolayer at low (L) and high (H) ...read more
Effect of ET-1 on glucose uptake in HIF-1 alpha -silenced astrocytes.Astrocytes were transfected with NT-siRNA or with HIF-1 alpha -siRNA. After 48 h, astrocytes were incubated in the absence (control, C) or presence of ...read more
FKBP51 increases expression of melanoma CSC markers. (a) FKBP51 silencing decreases ABCB5 levels. (Left) Normalized expression rates (arbitrary units (AU)) (mean±S.D.) of FKBP51 (black) and ABCB5 mRNA (grey) (n=3). ...read more
Tlk1-deficiency in mESCs causes a delay in the downregulation of core pluripotency factors upon differentiation. (A,C and E) Representative immunoblotting images showing Tlk1, Oct4, Sox2, and Nanog levels in Tlk1-KD ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, ZeSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IP, WB, ChIP, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

YAP1 Antibody - BSA Free Summary

Immunogen
This YAP1 Antibody was developed against a partial recombinant human YAP1 protein expressed in bacteria. [Uniprot: P46937], N-terminal GST fusion protein
Localization
Nuclear
Specificity
Expected reactivity based on immunogen homology: Isoform 4 (100%), Isoform 6 (100%)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunoblotting reported in scientific literature (PMID 28406163)
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 28581498)
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 2-10 ug
  • Knockdown Validated reported in scientific literature (PMID 28406163)
  • Knockout Validated
  • Simple Western 1:12.5
  • Western Blot 1:1000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
293 Whole Cell Lysate
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 2 Reviews rated 3.5
using
NB110-58358 in the following applications:

Publications
Read Publications using
NB110-58358 in the following applications:

Reactivity Notes

Use in Human reported in scientific literature (PMID:33737385). Use in Zebrafish reported in scientific literature (PMID:28350986).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for YAP1 Antibody - BSA Free

  • 65 kDa Yes-associated protein
  • COB1
  • Protein Yorkie Homolog
  • Transcriptional Coactivator YAP1
  • YAP
  • YAP1
  • YAP1-2gamma
  • YAP2
  • YAP2L
  • YAP65
  • YAP65yes-associated protein 2
  • Yes Associated Protein
  • Yes-associated protein 1
  • Yes-associated protein 1, 65kDa
  • yes-associated protein beta
  • yes-associated protein delta
  • Yes-Associated Protein YAP65 Homolog
  • Yes-Associated Protein
  • YKI
  • Yorkie Homolog

Background

The transcriptional coactivator Yes-associated protein (YAP), also known as Yes-associated protein 1 (YAP1), Yes-Associated Protein YAP65 Homolog, Protein Yorkie Homolog and YAP65 has a theoretical molecular weight of 65 kDa. The YAP1 gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. YAP1 shares homology with the WW domain of transcriptional co-activator with PDZ-binding motif (TAZ), which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors and is likely involved in protein-protein interaction. YAP has been identified as a core regulatory mechanism that blocks mammalian glial cell proliferation and cellular reprogramming following damage (1).

YAP plays a role in the development and progression of multiple cancers as a transcriptional regulator of the Hippo signaling pathway. YAP1 encodes a nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis to play a pivotal role in controlling cell growth and organ size and has emerged as a key player in tumor suppression (2,3). Deregulation of the Hippo pathway causes tumor formation and malignancy, with YAP being a key oncogenic driver in liver carcinogenesis (2) and may function as a potential target for cancer treatment (3).

References

1. Rueda, E. M., Hall, B. M., Hill, M. C., Swinton, P. G., Tong, X., Martin, J. F., & Poche, R. A. (2019). The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming. Cell Rep, 27(6), 1637-1649.e1636. doi:10.1016/j.celrep.2019.04.047

2. Liu, A. M., Xu, M. Z., Chen, J., Poon, R. T., & Luk, J. M. (2010). Targeting YAP and Hippo signaling pathway in liver cancer. Expert Opin Ther Targets, 14(8), 855-868. doi:10.1517/14728222.2010.499361

3.Ye, S., & Eisinger-Mathason, T. S. (2016). Targeting the Hippo pathway: Clinical implications and therapeutics. Pharmacol Res, 103, 270-278. doi:10.1016/j.phrs.2015.11.025

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for YAP1 Antibody (NB110-58358)(79)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Zebrafish.

We have publications tested in 11 applications: B/N, Chemotaxis, ICC/IF, IF/IHC, IHC, IHC-F, IHC-Fr, IHC-P, IP, KD, WB.


Filter By Application
B/N
(2)
Chemotaxis
(13)
ICC/IF
(15)
IF/IHC
(7)
IHC
(2)
IHC-F
(3)
IHC-Fr
(2)
IHC-P
(12)
IP
(6)
KD
(5)
WB
(32)
All Applications
Filter By Species
Human
(29)
Mouse
(30)
Rat
(1)
Canine
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 79. Show All 79 Publications.
Publications using NB110-58358 Applications Species
McCourt JL, Stearns-Reider KM, Mamsa H et al. Multi-omics analysis of sarcospan overexpression in mdx skeletal muscle reveals compensatory remodeling of cytoskeleton-matrix interactions that promote mechanotransduction pathways Skeletal Muscle 2023-01-06 [PMID: 36609344] (ICC/IF, IHC-Fr) ICC/IF, IHC-Fr
Sakabe M, Thompson M, Chen N et al. Inhibition of b 1-AR/G alpha s signaling promotes cardiomyocyte proliferation in juvenile mice through activation of RhoA-YAP axis eLife 2022-12-08 [PMID: 36479975] (WB, Mouse)

Details:
Dilution used in WB
WB Mouse
Grundl M Biochemical characterization of the MMB-Hippo crosstalk and its physiological relevance for heart development Thesis 2021-01-01
Sunderland A, Williams J, Andreou T et al. Biglycan and reduced glycolysis are associated with breast cancer cell dormancy in the brain Frontiers in Oncology 2023-06-29 [PMID: 37456245] (ICC/IF) ICC/IF
Karatsai O, Lehka L, Wojton D et al. Unconventional myosin VI in the heart: Involvement in cardiac dysfunction progressing with age Biochimica et biophysica acta. Molecular basis of disease 2023-05-09 [PMID: 37169038] (WB, Mouse) WB Mouse
Macfarlane E, Cavanagh L, Fong-Yee C et al. Deletion of the Chondrocyte Glucocorticoid Receptor Attenuates Cartilage Degradation through Suppression of Early Synovial Activation in Murine Posttraumatic Osteoarthritis Osteoarthritis and cartilage 2023-04-25 [PMID: 37105394]
Fetiva MC, Liss F, Gertzmann D et al. Oncogenic YAP mediates changes in chromatin accessibility and activity that drive cell cycle gene expression and cell migration Nucleic Acids Research 2023-05-22 [PMID: 36864753] (B/N) B/N
Meliambro K, Yang Y, de Cos M et al. KIBRA upregulation increases susceptibility to glomerular injury and correlates with kidney function decline JCI insight 2023-02-28 [PMID: 36853804] (IHC-Fr, ICC/IF, Mouse, Human) IHC-Fr, ICC/IF Mouse, Human
Banerjee P, Kumaravel S, Roy S et al. Conjugated Bile Acids Promote Lymphangiogenesis by Modulation of the Reactive Oxygen Species-p90RSK-Vascular Endothelial Growth Factor Receptor 3 Pathway Cells 2023-02-06 [PMID: 36831193] (ICC/IF, IHC, Human) ICC/IF, IHC Human
Wang EY. Transcriptional regulation by the Hippo signaling pathway in the liver. Thesis. 1905-07-09 (Chemotaxis, Mouse) Chemotaxis Mouse
Show All 79 Publications.

Reviews for YAP1 Antibody (NB110-58358) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Zebrafish.

Reviews using NB110-58358:
Filter by Applications
WB
(2)
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Human
(1)
Zebrafish
(1)
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Images Ratings Applications Species Date Details
Western Blot YAP1 NB110-58358
Enlarge
3
reviewed by:
Verified Customer
WB Human 02/04/2019
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Summary

ApplicationWestern Blot
Sample TestedHuman prostate cancer cell line (PC3)
SpeciesHuman
Lot-
Western Blot YAP1 NB110-58358
Enlarge
4
reviewed by:
Verified Customer
WB Zebrafish 01/24/2018
View

Summary

ApplicationWestern Blot
Sample TestedProtein Lysate
SpeciesZebrafish
Lotna

Comments

CommentsLysate prepared with RIPA buffer. Reducing agent was used. 70 deg C for 10 min. Ran on 4-12% bis tris gel at 200v for 40 min.Primary antibody was used at a dilution of 1:1000 and incubated overnight at 4C. Secondary antibody was RDyde 800 CW Goat anti-Rabbit IgG diluted 1:5000 incubated overnight at 4C. Detection method was Licor Odyssey detection.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for YAP1 Antibody (NB110-58358). (Showing 1 - 2 of 2 FAQs).

  1. I am trying to find a good antibody for YAP1 to use in a ChIP. The one that has been published and seems to work in ChIP was produced by Marius Sudol's lab. Is the YAP antibody NB110-58358 the one Marius Sudol sent you or did you produce it using his antigen?
    • You are correct. This antibody is from Sudol's lab, and yes it was produced directly by him.
  2. Our customer purchased NB110-58358 for IHC-P. The sample would be mouse skin. Now they face the problem of choosing which protocol of antigen retrieval is the best one to work with NB110-58358, since there are several listed on //www.novusbio.com/support/support-by-application/antigen-retrieval/protocol.html. Would you please help suggest?
    • To answer your customer's question, we do not have any protocol specific to NB110-58358 but as a general guideline we usually recommend using Citrate buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0) for antigen retrieving. 20 minutes incubation is usually sufficient in our experience.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on YAP1.


  Read full blog post.

YAP1 - a transcription co-activator and the downstream target of Hippo pathway
YAP1 (Yes-associated protein 1) is a  transcriptional co-activator which acts as a major effector of Hippo signaling pathway that regulates organ size/ tissue homeostasis and cell proliferation, and is an established oncogene (1).  Hippo sig...  Read full blog post.

mFluor Violet Conjugated Antibodies

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Verified Customer
02/04/2019
Application: WB
Species: Human

Verified Customer
01/24/2018
Application: WB
Species: Zebrafish

Bioinformatics

Gene Symbol YAP1