YAP1 Antibody

Knockout Validated: YAP1 Antibody [NB110-58358] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human YAP1 Polyclonal Antibody (Catalog # NB110-58358) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Immunocytochemistry/Immunofluorescence: YAP1 Antibody [NB110-58358] - Analysis in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (red) and DAPI (blue), respectively.

Western Blot: YAP1 Antibody [NB110-58358] - Analysis in transfected HEK 293 cell lysate.

Simple Western: YAP1 Antibody [NB110-58358] - Simple Western lane view shows a specific band for YAP1 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Immunohistochemistry-Paraffin: YAP1 Antibody [NB110-58358] - YAP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human YAP1 polyclonal Antibody (Catalog # NB110-58358) at 1:200 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclear and cytoplasm in trophoblast cells.

Knockout Validated: YAP1 Antibody [NB110-58358] - Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A specific band was detected for YAP1 at approximately 81 kDa (as indicated) using 50 ug/mL of Rabbit Anti-YAP1 Polyclonal Antibody (Catalog # NB110-58358). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Dr
• Applications: WB, Simple Western, ChIP, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

YAP1 Antibody Summary

• Immunogen: Recombinant human YAP1 protein expressed in bacteria. [Uniprot: P46937]
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Simple Western 1:12.5
  • Chromatin Immunoprecipitation
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 2-10 ug
  • Knockout Validated
• Application Notes: Western blot was performed with transfected HEK 293 lysates, where a band was detected at ~ 65 kDa.
  
  Use in Immunoblotting reported in scientific literature (PMID: 28406163).
  
  Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 28581498).
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  YAP1 Lysate (NBL1-17923)
  293 Lysate (NB800-PC6)
  Kidney Lysate (NB820-59231)
  Brain Lysate (NB820-59177)
  YAP1 Lysate (NBP2-65458)

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 29262338).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 15% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for YAP1 Antibody

• 65 kDa Yes-associated protein
• YAP
• YAP1
• YAP2
• YAP65
• YAP65yes-associated protein 2
• Yes-associated protein 1
• Yes-associated protein 1, 65kDa
• yes-associated protein beta
• yes-associated protein delta
• YKI
• Yorkie Homolog

Background

YAP1 (Yes-associated protein 1 or Yorkie homolog) is a transcriptional modulator that has been implicated in stem cell differentiation and the regulation of size of organs. It is a transcriptional regulator that can act both as a coactivator as well as a corepressor and is a crucial downstream target of Hippo signaling pathway that plays a pivotal role in restricting cellular proliferation and promoting apoptosis. STK3/MST2, STK4/MST1 and SAV1 activate LATS1/2-MOB1 complex by executing its phosphorylation which in turn phosphorylates and inactivates YAP1 oncoprotein as well as WWTR1/TAZ. LATS1/2 mediated phosphorylation of YAP1 inhibits its translocation into the nucleus to regulate genes responsible for cellular proliferation, death, and migration. TEAD transcription factors are essential for YAP1 to stimulate gene expression, cell growth, anchorage-independent growth, and induction of EMT. YAP1 is frequently overexpressed in certain malignancies including prostate and hepatocellular cancers where it drives the survival of tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for YAP1 Antibody (NB110-58358)(27)

Publications using NB110-58358

• Mueller kA, Glajch kE, Huizenga MN et al. Hippo Signaling Pathway Dysregulation in Human Huntington's Disease Brain and Neuronal Stem Cells. Sci Rep. Jul 27 2018 [PMID: 30054496] (IHC-Fr, Mouse)
• Liu M, Jiang k, Lin G et al. Ajuba inhibits hepatocellular carcinoma cell growth via targeting of B-catenin and YAP signaling and is regulated by E3 ligase Hakai through neddylation. J. Exp. Clin. Cancer Res. Jul 24 2018 [PMID: 30041665] (WB, Human)
• Cai J, Song X, Wang W et al. A RhoA-YAP-c-Myc signaling axis promotes the development of polycystic kidney disease Genes Dev. Jun 1 2018 [PMID: 29891559] (Mouse)
• Dehghanian F, Hojati Z, Esmaeili F, Masoudi-Nejad A. Network-based expression analyses and experimental validations revealed high co-expression between Yap1 and stem cell markers compared to differentiated cells. Genomics 2018 May 21 [PMID: 29775782]
• Artap S, Manderfield LJ, Smith CL et al. Endocardial Hippo signaling regulates myocardial growth and cardiogenesis Dev. Biol. May 1 2018 [PMID: 29727635] (ChIP)
• Xiao Y, Hill MC, Zhang M et al. Hippo Signaling Plays an Essential Role in Cell State Transitions during Cardiac Fibroblast Development. Dev. Cell Apr 23 2018 [PMID: 29689192] (ICC/IF, Mouse)
• Zheng Y, Liu B, Wang L et al. Homeostatic Control of Hpo/MST Kinase Activity through Autophosphorylation-Dependent Recruitment of the STRIPAK PP2A Phosphatase Complex. Cell Rep 2017 Dec 19 [PMID: 29262338] (WB, Drosophila)
• Leach JP, Heallen T, Zhang M et al. Hippo pathway deficiency reverses systolic heart failure after infarction. Nature Oct 12 2017 [PMID: 28976966] (WB, Human)
• Sarkar S, Bristow CA, Dey P et al. PRKCI promotes immune suppression in ovarian cancer Genes Dev. 2017 Jun 01 [PMID: 28698296] (Human)
• Morikawa Y, Heallen T, Leach J et al. Dystrophin-glycoprotein complex sequesters Yap to inhibit cardiomyocyte proliferation. Nature 2017 Jul 13 [PMID: 28581498] (IHC-Fr, Mouse)
• Bassat E, Mutlak YE, Genzelinakh A et al. The extracellular matrix protein agrin promotes heart regeneration in mice. Nature. Jul 13 2017 [PMID: 28581497] (ICC/IF, Mouse)
• Kim Y, Kim W, Song Y et al. Deubiquitinase YOD1 potentiates YAP/TAZ activities through enhancing ITCH stability Proc. Natl. Acad. Sci. U.S.A. 2017 May 02 [PMID: 28416659] (IHC, Human)
• Hirata H, Samsonov M, Sokabe M. Actomyosin contractility provokes contact inhibition in E-cadherin-ligated keratinocytes. Sci Rep. Apr 13 2017 [PMID: 28406163] (ICC/IF, IB, Human)
• Plubell DL, Wilmarth PA, Zhao Y et al. Extended multiplexing of TMT labeling reveals age and high fat diet specific proteome changes in mouse epididymal adipose tissue. Mol. Cell Proteomics. Mar 21 2017 [PMID: 28325852]
• Lin C, Yao E, Zhang K et al. YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis. Elife. Mar 21 2017 [PMID: 28323616] (ICC/IF, Mouse)
• Tao G, Kahr PC, Morikawa Y et al. Pitx2 promotes heart repair by activating the antioxidant response after cardiac injury. Nature. Jun 2 2016 [PMID: 27251288] (WB, ChIP, Mouse)
• Rizvi S, Yamada D, Hirsova P et al. A Hippo and Fibroblast Growth Factor Receptor Autocrine Pathway in Cholangiocarcinoma J. Biol. Chem. Jan 29 2016 [PMID: 26826125] (IHC-Fr, Human)
• Jung Kwang Hwa, McCarthy Ryan L, Zhou Chong et al. MicroRNA Regulates Hepatocytic Differentiation of Progenitor Cells by Targeting YAP1. Stem Cells (Dayton, Ohio) 2016 [PMID: 26731713] (WB, Human)
• Chen WC, Lin HH, Tang MJ et al. Matrix-Stiffness-Regulated Inverse Expression of Kruppel-Like Factor 5 and Kruppel-Like Factor 4 in the Pathogenesis of Renal Fibrosis Am. J. Pathol. 2015 Jul 23 [PMID: 26212907] (IHC-P, ICC/IF, Mouse)
  
  Details:
  YAP1 antibody used for ICC/IF and IHC-P on mouse samples. ICC/IF performed on primary mouse proximal tubular epithelial cells (mPTECs) (Fig 5), and IHC-P performed on mouse kidney sections from sham (control), UUO (unilateral ureteral obstruction), or UUO + BAPN at day 7 (Fig 6B).
• Wang EY. Transcriptional regulation by the Hippo signaling pathway in the liver. Thesis. Jul 9 1905 (ChIP, Mouse)
• Brusgard JL, Choe M, Chumsri S et al. RUNX2 and TAZ-dependent signaling pathways regulate soluble E-Cadherin levels and tumorsphere formation in breast cancer cells. Oncotarget 2015 Sep 29 [PMID: 26320173] (WB)
• Cai J, Maitra A, Anders RA et al. beta-Catenin destruction complex-independent regulation of Hippo-YAP signaling by APC in intestinal tumorigenesis. Genes Dev 2015 Jul 15 [PMID: 26193883] (WB)
• Chen Q, Zhang N, Xie R et al. Homeostatic control of Hippo signaling activity revealed by an endogenous activating mutation in YAP. Genes Dev 2015 Jun 15 [PMID: 26109051] (WB, Mouse)
• Karystinou A, Roelofs AJ, Neve A et al. Yes-associated protein (YAP) is a negative regulator of chondrogenesis in mesenchymal stem cells. Arthritis Res Ther 2015 [PMID: 26025096] (IHC, Mouse)
• Tsujiura M, Mazack V, Sudol M et al. Yes-Associated Protein (YAP) Modulates Oncogenic Features and Radiation Sensitivity in Endometrial Cancer. PLoS ONE. 2014 Jun 28 [PMID: 24972085] (IHC-P, WB, ICC/IF, Human)
• Kapoor A, Yao W, Ying H et al. Yap1 Activation Enables Bypass of Oncogenic Kras Addiction in Pancreatic Cancer. Cell. 2014 Jul 03 [PMID: 24954535] (IHC-P, WB, IP)
• Miao HL, Pan ZJ, Lei CJ et al. Knockdown of GPC3 inhibits the proliferation of Huh7 hepatocellular carcinoma cells through down-regulation of YAP. J Cell Biochem 2013 Mar [PMID: 23060277] (WB, Human)

Reviews for YAP1 Antibody (NB110-58358) (2)

Average Rating: 3.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Zebrafish
.

reviewed by: Anonymous WB Human 02/04/2019

Summary

• Application: Western Blot
• Sample Tested: Human prostate cancer cell line (PC3)
• Species: Human
• Lot: -

reviewed by: Anonymous WB Zebrafish
 01/24/2018

Summary

• Application: Western Blot
• Sample Tested: Protein Lysate
• Species: Zebrafish
• Lot: na

Comments

• Comments: Lysate prepared with RIPA buffer. Reducing agent was used. 70 deg C for 10 min. Ran on 4-12% bis tris gel at 200v for 40 min.Primary antibody was used at a dilution of 1:1000 and incubated overnight at 4C. Secondary antibody was RDyde 800 CW Goat anti-Rabbit IgG diluted 1:5000 incubated overnight at 4C. Detection method was Licor Odyssey detection.

FAQs for YAP1 Antibody (NB110-58358). (Showing 1 - 2 of 2 FAQs).

1. I am trying to find a good antibody for YAP1 to use in a ChIP. The one that has been published and seems to work in ChIP was produced by Marius Sudol's lab. Is the YAP antibody NB110-58358 the one Marius Sudol sent you or did you produce it using his antigen?
   • You are correct. This antibody is from Sudol's lab, and yes it was produced directly by him.
2. Our customer purchased NB110-58358 for IHC-P. The sample would be mouse skin. Now they face the problem of choosing which protocol of antigen retrieval is the best one to work with NB110-58358, since there are several listed on http://www.novusbio.com/support/support-by-application/antigen-retrieval/protocol.html. Would you please help suggest?
   • To answer your customer's question, we do not have any protocol specific to NB110-58358 but as a general guideline we usually recommend using Citrate buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0) for antigen retrieving. 20 minutes incubation is usually sufficient in our experience.

Bioinformatics

• Gene Symbol: YAP1