YAP1 Antibody

Images

 
Genetic Strategies: Knockout Validated: YAP1 Antibody [NB110-58358] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane ...read more
Immunocytochemistry/ Immunofluorescence: YAP1 Antibody [NB110-58358] - Analysis in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (red) and ...read more
Western Blot: YAP1 Antibody [NB110-58358] - Analysis in transfected HEK 293 cell lysate.
Simple Western: YAP1 Antibody [NB110-58358] - Simple Western lane view shows a specific band for YAP1 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation ...read more
Immunohistochemistry-Paraffin: YAP1 Antibody [NB110-58358] - YAP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human YAP1 polyclonal Antibody (Catalog # NB110-58358) at ...read more
Genetic Strategies: Knockout Validated: YAP1 Antibody [NB110-58358] - Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A ...read more

Product Details

Summary
Reactivity Hu, Mu, DrSpecies Glossary
Applications WB, Simple Western, ChIP, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

YAP1 Antibody Summary

Immunogen
Recombinant human YAP1 protein expressed in bacteria. [UniProt# P46937]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:12.5
  • Chromatin Immunoprecipitation
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 2-10 ug
  • Knockout Validated
Application Notes
This YAP1 antibody is useful for Immunocytochemistry/Immunofluorescence, Western blot, Immunoprecipitation, Chromatin Immunoprecipitation and Immunohistochemistry paraffin-embedded sections. The Western blot was performed with transfected HEK 293 lysates, where a band was detected approx. 65 kDa. Its use in ChIP application has been reported in PMID 20516196. Use in Immunoblotting reported in scientific literature (PMID 28406163). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 28581498). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
YAP1 Lysate (NBL1-17923)
293 Lysate (NB800-PC6)
Kidney Lysate (NB820-59231)
Brain Lysate (NB820-59177)
YAP1 Lysate (NBP2-65458)
Reviewed Applications
Read 1 Review rated 4
using
NB110-58358 in the following applications:

Publications
Read Publications using
NB110-58358 in the following applications:

  • 3 publications
  • IB
    1 publication
  • 6 publications
  • IHC
    2 publications
  • 3 publications
  • 3 publications
  • IP
    1 publication
  • WB
    11 publications

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 29262338).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 15% glycerol
Preservative
0.02% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for YAP1 Antibody

  • 65 kDa Yes-associated protein
  • YAP
  • YAP1
  • YAP2
  • YAP65
  • YAP65yes-associated protein 2
  • Yes-associated protein 1
  • Yes-associated protein 1, 65kDa
  • yes-associated protein beta
  • yes-associated protein delta
  • YKI
  • Yorkie Homolog

Background

YAP1 (Yes-associated protein 1 or Yorkie homolog) is a transcriptional modulator that has been implicated in stem cell differentiation and the regulation of size of organs. It is a transcriptional regulator that can act both as a coactivator as well as a corepressor and is a crucial downstream target of Hippo signaling pathway that plays a pivotal role in restricting cellular proliferation and promoting apoptosis. STK3/MST2, STK4/MST1 and SAV1 activate LATS1/2-MOB1 complex by executing its phosphorylation which in turn phosphorylates and inactivates YAP1 oncoprotein as well as WWTR1/TAZ. LATS1/2 mediated phosphorylation of YAP1 inhibits its translocation into the nucleus to regulate genes responsible for cellular proliferation, death, and migration. TEAD transcription factors are essential for YAP1 to stimulate gene expression, cell growth, anchorage-independent growth, and induction of EMT. YAP1 is frequently overexpressed in certain malignancies including prostate and hepatocellular cancers where it drives the survival of tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Dr
Applications: WB, Simple Western, ChIP, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO

Publications for YAP1 Antibody (NB110-58358)(27)

We have publications tested in 3 confirmed species: Human, Mouse, Drosophila.

We have publications tested in 8 applications: ChIP, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ChIP
(3)
IB
(1)
ICC/IF
(6)
IHC
(2)
IHC-Fr
(3)
IHC-P
(3)
IP
(1)
WB
(11)
All Applications
Filter By Species
Human
(9)
Mouse
(11)
Drosophila
(1)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using NB110-58358 Applications Species
Mueller kA, Glajch kE, Huizenga MN et al. Hippo Signaling Pathway Dysregulation in Human Huntington's Disease Brain and Neuronal Stem Cells. Sci Rep. Jul 27 2018 [PMID: 30054496] (IHC-Fr, Mouse) IHC-Fr Mouse
Wang EY. Transcriptional regulation by the Hippo signaling pathway in the liver. Thesis. Jul 9 1905 (ChIP, Mouse) ChIP Mouse
Liu M, Jiang k, Lin G et al. Ajuba inhibits hepatocellular carcinoma cell growth via targeting of B-catenin and YAP signaling and is regulated by E3 ligase Hakai through neddylation. J. Exp. Clin. Cancer Res. Jul 24 2018 [PMID: 30041665] (WB, Human) WB Human
Cai J, Song X, Wang W et al. A RhoA-YAP-c-Myc signaling axis promotes the development of polycystic kidney disease Genes Dev. Jun 1 2018 [PMID: 29891559] (Mouse) Mouse
Dehghanian F, Hojati Z, Esmaeili F, Masoudi-Nejad A. Network-based expression analyses and experimental validations revealed high co-expression between Yap1 and stem cell markers compared to differentiated cells. Genomics 2018 May 21 [PMID: 29775782]
Artap S, Manderfield LJ, Smith CL et al. Endocardial Hippo signaling regulates myocardial growth and cardiogenesis Dev. Biol. May 1 2018 [PMID: 29727635] (ChIP) ChIP
Xiao Y, Hill MC, Zhang M et al. Hippo Signaling Plays an Essential Role in Cell State Transitions during Cardiac Fibroblast Development. Dev. Cell Apr 23 2018 [PMID: 29689192] (ICC/IF, Mouse) ICC/IF Mouse
Zheng Y, Liu B, Wang L et al. Homeostatic Control of Hpo/MST Kinase Activity through Autophosphorylation-Dependent Recruitment of the STRIPAK PP2A Phosphatase Complex. Cell Rep 2017 Dec 19 [PMID: 29262338] (WB, Drosophila) WB Drosophila
Leach JP, Heallen T, Zhang M et al. Hippo pathway deficiency reverses systolic heart failure after infarction. Nature Oct 12 2017 [PMID: 28976966] (WB, Human) WB Human
Sarkar S, Bristow CA, Dey P et al. PRKCI promotes immune suppression in ovarian cancer Genes Dev. 2017 Jun 01 [PMID: 28698296] (Human) Human
Show All 27 Publications.

Review for YAP1 Antibody (NB110-58358) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Zebrafish
.

Reviews using NB110-58358:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Zebrafish
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot YAP1 NB110-58358
Enlarge
reviewed by:
Anonymous
WB Zebrafish
 01/24/2018
View

Summary

ApplicationWestern Blot
Sample TestedProtein Lysate
SpeciesZebrafish
Lotna

Comments

CommentsLysate prepared with RIPA buffer. Reducing agent was used. 70 deg C for 10 min. Ran on 4-12% bis tris gel at 200v for 40 min.Primary antibody was used at a dilution of 1:1000 and incubated overnight at 4C. Secondary antibody was RDyde 800 CW Goat anti-Rabbit IgG diluted 1:5000 incubated overnight at 4C. Detection method was Licor Odyssey detection.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for YAP1 Antibody (NB110-58358). (Showing 1 - 2 of 2 FAQs).

  1. I am trying to find a good antibody for YAP1 to use in a ChIP. The one that has been published and seems to work in ChIP was produced by Marius Sudol's lab. Is the YAP antibody NB110-58358 the one Marius Sudol sent you or did you produce it using his antigen?
    • You are correct. This antibody is from Sudol's lab, and yes it was produced directly by him.
  2. Our customer purchased NB110-58358 for IHC-P. The sample would be mouse skin. Now they face the problem of choosing which protocol of antigen retrieval is the best one to work with NB110-58358, since there are several listed on http://www.novusbio.com/support/support-by-application/antigen-retrieval/protocol.html. Would you please help suggest?
    • To answer your customer's question, we do not have any protocol specific to NB110-58358 but as a general guideline we usually recommend using Citrate buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0) for antigen retrieving. 20 minutes incubation is usually sufficient in our experience.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for YAP1 Antibody (NB110-58358)

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Blogs on YAP1.

YAP1 - a transcription co-activator and the downstream target of Hippo pathway
YAP1 (Yes-associated protein 1) is a  transcriptional co-activator which acts as a major effector of Hippo signaling pathway that regulates organ size/ tissue homeostasis and cell proliferation, and is an established oncogene (1).  Hippo sig...  Read full blog post.

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Recent Reviews

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Anonymous
01/24/2018
Application: WB
Species: Zebrafish

Bioinformatics

Gene Symbol YAP1