YAP1 Antibody

Knockout Validated: YAP1 Antibody [NB110-58358] - Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A specific band was detected for YAP1 at approximately 81 kDa (as indicated) using 50 ug/mL of Rabbit Anti-YAP1 Polyclonal Antibody (Catalog # NB110-58358). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated: YAP1 Antibody [NB110-58358] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human YAP1 Polyclonal Antibody (Catalog # NB110-58358) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Immunocytochemistry/Immunofluorescence: YAP1 Antibody [NB110-58358] - Analysis in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (red) and DAPI (blue), respectively.

Immunohistochemistry-Paraffin: YAP1 Antibody [NB110-58358] - YAP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human YAP1 polyclonal Antibody (Catalog # NB110-58358) at 1:200 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclear and cytoplasm in trophoblast cells.

Western Blot: YAP1 Antibody [NB110-58358] - Actomyosin activity inhibits beta-catenin- and YAP-driven proliferation of confluent keratinocytes. Effects of cell density and actomyosin activity on YAP phosphorylation. HaCaT cells cultured for 40 h under the sparse and confluent conditions were treated with 100 uM blebbistatin (Blebb) or DMSO (for control) for 6 h, and then lysed and immunoblotted for Ser127-phosphorylated YAP (pYAP), YAP, beta-catenin and actin. Similar results were obtained in two independent experiments. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep46326), licensed under a CC-BY licence.

Western Blot: YAP1 Antibody [NB110-58358] - Analysis in transfected HEK 293 cell lysate using YAP1 antibody. Observed molecular weight 75 kDa.

Western Blot: YAP1 Antibody [NB110-58358] - Consequences of ORP5 and ORP8 knockdown on downstream MAPK and PI3K/AKT signaling. Protein from MOH parental, single and double ORP knockdowns as well as cells transfected with empty vector control (pLKO.1) were harvested, and 20 ug was subjected to SDS-PAGE and used for Western blotting. Image collected and cropped by CiteAb from the following publication (//www.life-science-alliance.org/lookup/doi/10.26508/lsa.201900431), licensed under a CC-BY licence.

Simple Western: YAP1 Antibody [NB110-58358] - Simple Western lane view shows a specific band for YAP1 in 0.1 mg/ml of HeLa lysate. Observed molecular weight is 75 kDa. This experiment was performed under reducing conditions using the 12-230kDa separation system.

• Reactivity: Hu, Mu, Dr
• Applications: WB, Simple Western, ChIP, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

YAP1 Antibody Summary

• Immunogen: This YAP1 Antibody was developed against a partial recombinant human YAP1 protein expressed in bacteria. [Uniprot: P46937], N-terminal GST fusion protein
• Localization: Nuclear
• Specificity: Expected reactivity based on immunogen homology: Isoform 4 (100%), Isoform 6 (100%)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Simple Western 1:12.5
  • Chromatin Immunoprecipitation
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 2-10 ug
  • Knockdown Validated
  • Knockout Validated
• Application Notes: Use in Immunoblotting reported in scientific literature (PMID: 28406163). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 28581498). Use in Knockdown Validated reported in scientific literature (PMID:28406163). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  YAP1 Overexpression Lysate
  293 Whole Cell Lysate
  Human Kidney Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)
  YAP1 Knockout HeLa Cell Lysate

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 29262338).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for YAP1 Antibody

• 65 kDa Yes-associated protein
• COB1
• Protein Yorkie Homolog
• Transcriptional Coactivator YAP1
• YAP
• YAP1
• YAP1-2gamma
• YAP2
• YAP2L
• YAP65
• YAP65yes-associated protein 2
• Yes Associated Protein
• Yes-associated protein 1
• Yes-associated protein 1, 65kDa
• yes-associated protein beta
• yes-associated protein delta
• Yes-Associated Protein YAP65 Homolog
• Yes-Associated Protein
• YKI
• Yorkie Homolog

Background

The transcriptional coactivator Yes-associated protein (YAP), also known as Yes-associated protein 1 (YAP1), Yes-Associated Protein YAP65 Homolog, Protein Yorkie Homolog and YAP65 has a theoretical molecular weight of 65 kDa. The YAP1 gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. YAP1 shares homology with the WW domain of transcriptional co-activator with PDZ-binding motif (TAZ), which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors and is likely involved in protein-protein interaction. YAP has been identified as a core regulatory mechanism that blocks mammalian glial cell proliferation and cellular reprogramming following damage (1).

YAP plays a role in the development and progression of multiple cancers as a transcriptional regulator of the Hippo signaling pathway. YAP1 encodes a nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis to play a pivotal role in controlling cell growth and organ size and has emerged as a key player in tumor suppression (2,3). Deregulation of the Hippo pathway causes tumor formation and malignancy, with YAP being a key oncogenic driver in liver carcinogenesis (2) and may function as a potential target for cancer treatment (3).

References

1. Rueda, E. M., Hall, B. M., Hill, M. C., Swinton, P. G., Tong, X., Martin, J. F., & Poche, R. A. (2019). The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming. Cell Rep, 27(6), 1637-1649.e1636. doi:10.1016/j.celrep.2019.04.047

2. Liu, A. M., Xu, M. Z., Chen, J., Poon, R. T., & Luk, J. M. (2010). Targeting YAP and Hippo signaling pathway in liver cancer. Expert Opin Ther Targets, 14(8), 855-868. doi:10.1517/14728222.2010.499361

3.Ye, S., & Eisinger-Mathason, T. S. (2016). Targeting the Hippo pathway: Clinical implications and therapeutics. Pharmacol Res, 103, 270-278. doi:10.1016/j.phrs.2015.11.025

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for YAP1 Antibody (NB110-58358)(48)

Publications using NB110-58358

• He Q, Gao Y, Zhou L et al. Deficiency of Yes-Associated Protein Induces Cataract in Mice Aging and disease Jul 9 1905 (Mouse)
• Wang EY. Transcriptional regulation by the Hippo signaling pathway in the liver. Thesis. Jul 9 1905 (ChIP, Mouse)
• Imam Aliagan A, Madungwe N, Tombo N et al. Chronic GPER1 Activation Protects Against Oxidative Stress-Induced Cardiomyoblast Death via Preservation of Mitochondrial Integrity and Deactivation of Mammalian Sterile-20-Like Kinase/Yes-Associated Protein Pathway Front. Endocrinol. Oct 19 2020 [PMID: 33193095] (ICC/IF, Rat)
• Gao C, Hu W, Liu F et al. Aldo-keto reductase family 1 member B induces aortic valve calcification by activating hippo signaling in valvular interstitial cells J Mol Cell Cardiol Oct 9 2020 [PMID: 33045251] (WB, ChIP, Human)
• Aharonov A, Shakked A, Umansky KB, Savidor A ERBB2 drives YAP activation and EMT-like processes during cardiac regeneration bioRxiv Jan 1 2020 (IP, Mouse)
• Lorenzo-Martin LF, Fernandez-Parejo N, Menacho-Marquez M et al. VAV2 signaling promotes regenerative proliferation in both cutaneous and head and neck squamous cell carcinoma Nat Commun Sep 22 2020 [PMID: 32963234] (IHC-P, Mouse)
• Chakravarti D, Hu B, Mao X et al. Telomere dysfunction activates YAP1 to drive tissue inflammation Nat Commun Sep 21 2020 [PMID: 32958778] (ICC/IF, WB, Mouse)
• Yamashita A, Yoshitomi H, Kihara S et al. Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells Stem Cells Transl Med Aug 21 2020 [PMID: 32822104] (WB, KD, Human)
• Wilson KD, Ameen M, Guo H et al. Endogenous Retrovirus-Derived lncRNA BANCR Promotes Cardiomyocyte Migration in Humans and Non-human Primates Dev. Cell Jul 28 2020 [PMID: 32763147]
• Zhang X, Liu P, Shang Y et al. Metformin and LW6 impairs pancreatic cancer cells and reduces nuclear localization of YAP1 J Cancer. Dec 6 2019 [PMID: 31897243] (WB, ICC/IF, Human, Mouse)
• Liu Z, Qian M, Tang X, et al. The beta-catenin/YAP signaling axis is a key regulator of melanoma-associated fibroblasts Signal Transduct Target Ther. Dec 24 2019 [PMID: 31885878] (WB, KD, Human)
• Lefort S, Tan S, Balani S et al. Initiation of human mammary cell tumorigenesis by mutant KRAS requires YAP inactivation Oncogene. Nov 26 2019 [PMID: 31772328] (IHC-P, WB, Mouse)
• St-Jean G, Tsoi M et al. Lats1 and Lats2 are required for the maintenance of multipotency in the Mullerian duct mesenchyme. Development Oct 18 2019 [PMID: 31575647] (ChIP, Mouse)
  
  Details:
  Meriones unguiculatus (Mongolian gerbil)
• Kattan WE, Chen W, Ma X et al. Targeting plasma membrane phosphatidylserine content to inhibit oncogenic KRAS function Life Sci Alliance. Oct 1 2019 [PMID: 31451509] (WB, Human)
• Pattschull G, Walz S, Grundl M et al. The Myb-MuvB Complex Is Required for YAP-Dependent Transcription of Mitotic Genes Cell Rep Jun 18 2019 [PMID: 31216474] (IP, Human)
• Chen P, Zhao D, Li J et al. Symbiotic Macrophage-Glioma Cell Interactions Reveal Synthetic Lethality in PTEN-Null Glioma Cancer Cell Jun 10 2019 [PMID: 31185211]
• Flentke GR, Baulch JW, Berres ME et al. The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming Cell Rep. May 7 2019 [PMID: 31067451] (ChIP, Mouse)
• He Q, Gao Y, Wang, T et al. Deficiency of Yes-Associated Protein Induces Cataract in Mice Aging Dis. 2019 Apr 1 [PMID: 31011480] (IHC-F, IHC-P, WB, KD, Mouse)
• Ma R, Ren JM, Li P et al. Activated YAP causes renal damage of type 2 diabetic nephropathy Eur Rev Med Pharmacol Sci. Jan 1 2019 [PMID: 30720184] (IHC, Human)
• Mueller kA, Glajch kE, Huizenga MN et al. Hippo Signaling Pathway Dysregulation in Human Huntington's Disease Brain and Neuronal Stem Cells. Sci Rep. Jul 27 2018 [PMID: 30054496] (IHC-F, Mouse)
• Liu M, Jiang k, Lin G et al. Ajuba inhibits hepatocellular carcinoma cell growth via targeting of B-catenin and YAP signaling and is regulated by E3 ligase Hakai through neddylation. J Exp Clin Cancer Res. Jul 24 2018 [PMID: 30041665] (WB, KD, Human)
• Frank S R, Kollmann C P et al. p190 RhoGAP promotes contact inhibition in epithelial cells by repressing YAP activity. J Cell Biol Mar 9 2018 [PMID: 29934311] (WB, Canine)
• Cai J, Song X, Wang W et al. A RhoA-YAP-c-Myc signaling axis promotes the development of polycystic kidney disease Genes Dev. Jun 1 2018 [PMID: 29891559] (ChIP, Human, Mouse)
• Dehghanian F, Hojati Z, Esmaeili F, Masoudi-Nejad A. Network-based expression analyses and experimental validations revealed high co-expression between Yap1 and stem cell markers compared to differentiated cells. Genomics. 2018 May 21 [PMID: 29775782] (WB, Mouse)
• Artap S, Manderfield LJ, Smith CL et al. Endocardial Hippo signaling regulates myocardial growth and cardiogenesis Dev Biol. May 1 2018 [PMID: 29727635] (ChIP, Human)
• Xiao Y, Hill MC, Zhang M et al. Hippo Signaling Plays an Essential Role in Cell State Transitions during Cardiac Fibroblast Development. Dev Cell. Apr 23 2018 [PMID: 29689192] (ChIP, Mouse)
• Zheng Y, Liu B, Wang L et al. Homeostatic Control of Hpo/MST Kinase Activity through Autophosphorylation-Dependent Recruitment of the STRIPAK PP2A Phosphatase Complex. Cell Rep. 2017 Dec 19 [PMID: 29262338] (ICC/IF, Human)
• Meliambro K, Wong JS, Ray J et al. JBC Papers in Press. Published on October 5, 2017 as Manuscript M117. 819029 J Biol Chem. 2017 Oct 05 [PMID: 28982981] (IHC, Human, Mouse)
• Leach JP, Heallen T, Zhang M et al. Hippo pathway deficiency reverses systolic heart failure after infarction. Nature. Oct 12 2017 [PMID: 28976966] (WB, Human)
• Sarkar S, Bristow CA, Dey P et al. PRKCI promotes immune suppression in ovarian cancer Genes Dev. 2017 Jun 01 [PMID: 28698296] (IHC-P, Mouse)
• Morikawa Y, Heallen T, Leach J et al. Dystrophin-glycoprotein complex sequesters Yap to inhibit cardiomyocyte proliferation. Nature. 2017 Jul 13 [PMID: 28581498] (IHC, WB, IP, Mouse)
• Bassat E, Mutlak YE, Genzelinakh A et al. The extracellular matrix protein agrin promotes heart regeneration in mice. Nature. Jul 13 2017 [PMID: 28581497] (IHC-P, Mouse)
• Kim Y, Kim W, Song Y et al. Deubiquitinase YOD1 potentiates YAP/TAZ activities through enhancing ITCH stability Proc Natl Acad Sci U S A. 2017 May 02 [PMID: 28416659] (IHC, Human)
• Hirata H, Samsonov M, Sokabe M. Actomyosin contractility provokes contact inhibition in E-cadherin-ligated keratinocytes. Sci Rep. Apr 13 2017 [PMID: 28406163] (KD, ICC/IF, WB, Human)
• Nakajima H, Yamamoto K et al. Flow-Dependent Endothelial YAP Regulation Contributes to Vessel Maintenance. Dev Cell Mar 27 2017 [PMID: 28350986] (IP, WB, Zebrafish, Human)
• Plubell DL, Wilmarth PA, Zhao Y et al. Extended multiplexing of TMT labeling reveals age and high fat diet specific proteome changes in mouse epididymal adipose tissue. Mol Cell Proteomics. Mar 21 2017 [PMID: 28325852] (WB, Mouse)
• Lin C, Yao E, Zhang K et al. YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis. Elife. Mar 21 2017 [PMID: 28323616] (IHC-P, IP, Mouse)
• Tao G, Kahr PC, Morikawa Y et al. Pitx2 promotes heart repair by activating the antioxidant response after cardiac injury. Nature. Jun 2 2016 [PMID: 27251288] (WB, IP, ChIP, Mouse)
• Rizvi S, Yamada D, Hirsova P et al. A Hippo and Fibroblast Growth Factor Receptor Autocrine Pathway in Cholangiocarcinoma J Biol Chem. Jan 29 2016 [PMID: 26826125] (WB, IHC-F, IHC-P, Mouse, Human)
• Jung Kwang Hwa, McCarthy Ryan L, Zhou Chong et al. MicroRNA Regulates Hepatocytic Differentiation of Progenitor Cells by Targeting YAP1. Stem Cells 2016 Feb 1 [PMID: 26731713] (WB, Human)
• Brusgard JL, Choe M, Chumsri S et al. RUNX2 and TAZ-dependent signaling pathways regulate soluble E-Cadherin levels and tumorsphere formation in breast cancer cells. Oncotarget. 2015 Sep 29 [PMID: 26320173] (WB, Human)
• Chen WC, Lin HH, Tang MJ et al. Matrix-Stiffness-Regulated Inverse Expression of Kruppel-Like Factor 5 and Kruppel-Like Factor 4 in the Pathogenesis of Renal Fibrosis Am J Pathol. 2015 Jul 23 [PMID: 26212907] (IHC, ICC/IF, Mouse)
• Cai J, Maitra A, Anders RA et al. beta-Catenin destruction complex-independent regulation of Hippo-YAP signaling by APC in intestinal tumorigenesis. Genes Dev. 2015 Jul 15 [PMID: 26193883] (WB, Human)
• Chen Q, Zhang N, Xie R et al. Homeostatic control of Hippo signaling activity revealed by an endogenous activating mutation in YAP. Genes Dev. 2015 Jun 15 [PMID: 26109051] (ICC/IF, IHC-P, WB, Mouse)
• Karystinou A, Roelofs AJ, Neve A et al. Yes-associated protein (YAP) is a negative regulator of chondrogenesis in mesenchymal stem cells. Arthritis Res Ther. 2015 May 30 [PMID: 26025096] (IHC-P, Mouse)
• Tsujiura M, Mazack V, Sudol M et al. Yes-Associated Protein (YAP) Modulates Oncogenic Features and Radiation Sensitivity in Endometrial Cancer. PLoS One 2014 Jun 28 [PMID: 24972085] (IHC-P, WB, Human)
• Kapoor A, Yao W, Ying H et al. Yap1 Activation Enables Bypass of Oncogenic Kras Addiction in Pancreatic Cancer. Cell 2014 Jul 03 [PMID: 24954535] (WB, IHC-P, IP, Mouse)
• Miao HL, Pan ZJ, Lei CJ et al. Knockdown of GPC3 inhibits the proliferation of Huh7 hepatocellular carcinoma cells through down-regulation of YAP. J Cell Biochem. 2013 Mar [PMID: 23060277] (WB, Human)

Reviews for YAP1 Antibody (NB110-58358) (2)

Average Rating: 3.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Zebrafish
.

reviewed by: Anonymous WB Human 02/04/2019

Summary

• Application: Western Blot
• Sample Tested: Human prostate cancer cell line (PC3)
• Species: Human
• Lot: -

reviewed by: Anonymous WB Zebrafish
 01/24/2018

Summary

• Application: Western Blot
• Sample Tested: Protein Lysate
• Species: Zebrafish
• Lot: na

Comments

• Comments: Lysate prepared with RIPA buffer. Reducing agent was used. 70 deg C for 10 min. Ran on 4-12% bis tris gel at 200v for 40 min.Primary antibody was used at a dilution of 1:1000 and incubated overnight at 4C. Secondary antibody was RDyde 800 CW Goat anti-Rabbit IgG diluted 1:5000 incubated overnight at 4C. Detection method was Licor Odyssey detection.

FAQs for YAP1 Antibody (NB110-58358). (Showing 1 - 2 of 2 FAQs).

1. I am trying to find a good antibody for YAP1 to use in a ChIP. The one that has been published and seems to work in ChIP was produced by Marius Sudol's lab. Is the YAP antibody NB110-58358 the one Marius Sudol sent you or did you produce it using his antigen?
   • You are correct. This antibody is from Sudol's lab, and yes it was produced directly by him.
2. Our customer purchased NB110-58358 for IHC-P. The sample would be mouse skin. Now they face the problem of choosing which protocol of antigen retrieval is the best one to work with NB110-58358, since there are several listed on http://www.novusbio.com/support/support-by-application/antigen-retrieval/protocol.html. Would you please help suggest?
   • To answer your customer's question, we do not have any protocol specific to NB110-58358 but as a general guideline we usually recommend using Citrate buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0) for antigen retrieving. 20 minutes incubation is usually sufficient in our experience.

Bioinformatics

• Gene Symbol: YAP1