Immunogen | This YAP1 Antibody was developed against a partial recombinant human YAP1 protein expressed in bacteria. [Uniprot: P46937], N-terminal GST fusion protein |
Localization | Nuclear |
Specificity | Expected reactivity based on immunogen homology: Isoform 4 (100%), Isoform 6 (100%) |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | YAP1 |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Use in Immunoblotting reported in scientific literature (PMID: 28406163). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 28581498). Use in Knockdown Validated reported in scientific literature (PMID:28406163). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
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Theoretical MW | 48 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
Buffer | PBS |
Preservative | 0.02% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Immunogen affinity purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||||
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Enlarge |
reviewed by:
Anonymous |
WB | Human | 02/04/2019 |
Summary
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Enlarge |
reviewed by:
Anonymous |
WB | Zebrafish
 | 01/24/2018 |
Summary
Comments
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YAP1 Overexpression Lysate | |
293 Whole Cell Lysate | |
Human Kidney Whole Tissue Lysate (Adult Whole Normal) | |
Human Brain Whole Tissue Lysate (Adult Whole Normal) | |
YAP1 Knockout HeLa Cell Lysate |
Secondary Antibodies |
Isotype Controls |
Alexa Fluor 350 | NB110-58358AF350 | |
Alexa Fluor 532 | NB110-58358AF532 | |
Alexa Fluor 594 | NB110-58358AF594 | |
Alexa Fluor 750 | NB110-58358AF750 | |
DyLight 350 | NB110-58358UV | |
DyLight 594 | NB110-58358DL594 | |
DyLight 680 | NB110-58358FR | |
DyLight 755 | NB110-58358IR | |
Janelia Fluor 549 | NB110-58358JF549 | |
Janelia Fluor 646 | NB110-58358JF646 |
Diseases for YAP1 Antibody (NB110-58358)Discover more about diseases related to YAP1 Antibody (NB110-58358).
| Pathways for YAP1 Antibody (NB110-58358)View related products by pathway.
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PTMs for YAP1 Antibody (NB110-58358)Learn more about PTMs related to YAP1 Antibody (NB110-58358).
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YAP1 - a transcription co-activator and the downstream target of Hippo pathway YAP1 (Yes-associated protein 1) is a transcriptional co-activator which acts as a major effector of Hippo signaling pathway that regulates organ size/ tissue homeostasis and cell proliferation, and is an established oncogene (1). Hippo sig... Read full blog post. |
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Anonymous 02/04/2019 |
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Application: | WB | |
Species: | Human |
Anonymous 01/24/2018 |
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Application: | WB | |
Species: | Zebrafish
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Gene Symbol | YAP1 |