Genetic Strategies: Knockout Validated: YAP1 Antibody [NB110-58358] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane ...read more
Immunocytochemistry/ Immunofluorescence: YAP1 Antibody [NB110-58358] - Analysis in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (red) and ...read more
Western Blot: YAP1 Antibody [NB110-58358] - Analysis in transfected HEK 293 cell lysate.
Simple Western: YAP1 Antibody [NB110-58358] - Simple Western lane view shows a specific band for YAP1 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation ...read more
Genetic Strategies: Knockout Validated: YAP1 Antibody [NB110-58358] - Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A ...read more
This YAP1 antibody is useful for Immunocytochemistry/Immunofluorescence, Western blot, Immunoprecipitation, Chromatin Immunoprecipitation and Immunohistochemistry paraffin-embedded sections. The Western blot was performed with transfected HEK 293 lysates, where a band was detected approx. 65 kDa. Its use in ChIP application has been reported in PMID 20516196. Use in Immunoblotting reported in scientific literature (PMID 28406163). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 28581498). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
65 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Drosophila reactivity reported in scientific literature (PMID: 29262338).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 15% glycerol
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for YAP1 Antibody
65 kDa Yes-associated protein
YAP65yes-associated protein 2
Yes-associated protein 1
Yes-associated protein 1, 65kDa
yes-associated protein beta
yes-associated protein delta
YAP1 (Yes-associated protein 1 or Yorkie homolog) is a transcriptional modulator that has been implicated in stem cell differentiation and the regulation of size of organs. It is a transcriptional regulator that can act both as a coactivator as well as a corepressor and is a crucial downstream target of Hippo signaling pathway that plays a pivotal role in restricting cellular proliferation and promoting apoptosis. STK3/MST2, STK4/MST1 and SAV1 activate LATS1/2-MOB1 complex by executing its phosphorylation which in turn phosphorylates and inactivates YAP1 oncoprotein as well as WWTR1/TAZ. LATS1/2 mediated phosphorylation of YAP1 inhibits its translocation into the nucleus to regulate genes responsible for cellular proliferation, death, and migration. TEAD transcription factors are essential for YAP1 to stimulate gene expression, cell growth, anchorage-independent growth, and induction of EMT. YAP1 is frequently overexpressed in certain malignancies including prostate and hepatocellular cancers where it drives the survival of tumors.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Lysate prepared with RIPA buffer. Reducing agent was used. 70 deg C for 10 min. Ran on 4-12% bis tris gel at 200v for 40 min.Primary antibody was used at a dilution of 1:1000 and incubated overnight at 4C. Secondary antibody was RDyde 800 CW Goat anti-Rabbit IgG diluted 1:5000 incubated overnight at 4C. Detection method was Licor Odyssey detection.
Product General Protocols
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FAQs for YAP1 Antibody (NB110-58358). (Showing 1 - 2 of 2 FAQs).
I am trying to find a good antibody for YAP1 to use in a ChIP. The one that has been published and seems to work in ChIP was produced by Marius Sudol's lab. Is the YAP antibody NB110-58358 the one Marius Sudol sent you or did you produce it using his antigen?
You are correct. This antibody is from Sudol's lab, and yes it was produced directly by him.
Our customer purchased NB110-58358 for IHC-P. The sample would be mouse skin. Now they face the problem of choosing which protocol of antigen retrieval is the best one to work with NB110-58358, since there are several listed on http://www.novusbio.com/support/support-by-application/antigen-retrieval/protocol.html. Would you please help suggest?
To answer your customer's question, we do not have any protocol specific to NB110-58358 but as a general guideline we usually recommend using Citrate buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0) for antigen retrieving. 20 minutes incubation is usually sufficient in our experience.
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