YAP1 Antibody

Images

 
Genetic Strategies: Knockout Validated: YAP1 Antibody [NB110-58358] - Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A ...read more
Genetic Strategies: Knockout Validated: YAP1 Antibody [NB110-58358] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane ...read more
Immunocytochemistry/ Immunofluorescence: YAP1 Antibody [NB110-58358] - Analysis in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (red) and ...read more
Immunohistochemistry-Paraffin: YAP1 Antibody [NB110-58358] - YAP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human YAP1 polyclonal Antibody (Catalog # NB110-58358) at ...read more
Western Blot: YAP1 Antibody [NB110-58358] - Actomyosin activity inhibits beta-catenin- and YAP-driven proliferation of confluent keratinocytes. Effects of cell density and actomyosin activity on YAP phosphorylation. ...read more
Western Blot: YAP1 Antibody [NB110-58358] - Analysis in transfected HEK 293 cell lysate using YAP1 antibody. Observed molecular weight 75 kDa.
Western Blot: YAP1 Antibody [NB110-58358] - Consequences of ORP5 and ORP8 knockdown on downstream MAPK and PI3K/AKT signaling. Protein from MOH parental, single and double ORP knockdowns as well as cells transfected ...read more
Simple Western: YAP1 Antibody [NB110-58358] - Simple Western lane view shows a specific band for YAP1 in 0.1 mg/ml of HeLa lysate. Observed molecular weight is 75 kDa. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, DrSpecies Glossary
Applications WB, Simple Western, ChIP, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

YAP1 Antibody Summary

Immunogen
This YAP1 Antibody was developed against a partial recombinant human YAP1 protein expressed in bacteria. [Uniprot: P46937], N-terminal GST fusion protein
Localization
Nuclear
Specificity
Expected reactivity based on immunogen homology: Isoform 4 (100%), Isoform 6 (100%)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:12.5
  • Chromatin Immunoprecipitation
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 2-10 ug
  • Knockdown Validated
  • Knockout Validated
Application Notes
Use in Immunoblotting reported in scientific literature (PMID: 28406163). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 28581498). Use in Knockdown Validated reported in scientific literature (PMID:28406163). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
YAP1 Overexpression Lysate
293 Whole Cell Lysate
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
YAP1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 3.5
using
NB110-58358 in the following applications:

Publications
Read Publications using
NB110-58358 in the following applications:

  • 8 publications
  • 7 publications
  • IHC
    5 publications
  • 3 publications
  • 11 publications
  • IP
    7 publications
  • KD
    5 publications
  • WB
    25 publications

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 29262338).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for YAP1 Antibody

  • 65 kDa Yes-associated protein
  • COB1
  • Protein Yorkie Homolog
  • Transcriptional Coactivator YAP1
  • YAP
  • YAP1
  • YAP1-2gamma
  • YAP2
  • YAP2L
  • YAP65
  • YAP65yes-associated protein 2
  • Yes Associated Protein
  • Yes-associated protein 1
  • Yes-associated protein 1, 65kDa
  • yes-associated protein beta
  • yes-associated protein delta
  • Yes-Associated Protein YAP65 Homolog
  • Yes-Associated Protein
  • YKI
  • Yorkie Homolog

Background

The transcriptional coactivator Yes-associated protein (YAP), also known as Yes-associated protein 1 (YAP1), Yes-Associated Protein YAP65 Homolog, Protein Yorkie Homolog and YAP65 has a theoretical molecular weight of 65 kDa. The YAP1 gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. YAP1 shares homology with the WW domain of transcriptional co-activator with PDZ-binding motif (TAZ), which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors and is likely involved in protein-protein interaction. YAP has been identified as a core regulatory mechanism that blocks mammalian glial cell proliferation and cellular reprogramming following damage (1).

YAP plays a role in the development and progression of multiple cancers as a transcriptional regulator of the Hippo signaling pathway. YAP1 encodes a nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis to play a pivotal role in controlling cell growth and organ size and has emerged as a key player in tumor suppression (2,3). Deregulation of the Hippo pathway causes tumor formation and malignancy, with YAP being a key oncogenic driver in liver carcinogenesis (2) and may function as a potential target for cancer treatment (3).

References

1. Rueda, E. M., Hall, B. M., Hill, M. C., Swinton, P. G., Tong, X., Martin, J. F., & Poche, R. A. (2019). The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming. Cell Rep, 27(6), 1637-1649.e1636. doi:10.1016/j.celrep.2019.04.047

2. Liu, A. M., Xu, M. Z., Chen, J., Poon, R. T., & Luk, J. M. (2010). Targeting YAP and Hippo signaling pathway in liver cancer. Expert Opin Ther Targets, 14(8), 855-868. doi:10.1517/14728222.2010.499361

3.Ye, S., & Eisinger-Mathason, T. S. (2016). Targeting the Hippo pathway: Clinical implications and therapeutics. Pharmacol Res, 103, 270-278. doi:10.1016/j.phrs.2015.11.025

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Dr
Applications: WB, Simple Western, ChIP, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO

Publications for YAP1 Antibody (NB110-58358)(48)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Zebrafish.

We have publications tested in 8 applications: ChIP, ICC/IF, IHC, IHC-F, IHC-P, IP, KD, WB.


Filter By Application
ChIP
(8)
ICC/IF
(7)
IHC
(5)
IHC-F
(3)
IHC-P
(11)
IP
(7)
KD
(5)
WB
(25)
All Applications
Filter By Species
Human
(22)
Mouse
(26)
Rat
(1)
Canine
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 48. Show All 48 Publications.
Publications using NB110-58358 Applications Species
He Q, Gao Y, Zhou L et al. Deficiency of Yes-Associated Protein Induces Cataract in Mice Aging and disease Jul 9 1905 (Mouse) Mouse
Wang EY. Transcriptional regulation by the Hippo signaling pathway in the liver. Thesis. Jul 9 1905 (ChIP, Mouse) ChIP Mouse
Imam Aliagan A, Madungwe N, Tombo N et al. Chronic GPER1 Activation Protects Against Oxidative Stress-Induced Cardiomyoblast Death via Preservation of Mitochondrial Integrity and Deactivation of Mammalian Sterile-20-Like Kinase/Yes-Associated Protein Pathway Front. Endocrinol. Oct 19 2020 [PMID: 33193095] (ICC/IF, Rat) ICC/IF Rat
Gao C, Hu W, Liu F et al. Aldo-keto reductase family 1 member B induces aortic valve calcification by activating hippo signaling in valvular interstitial cells J Mol Cell Cardiol Oct 9 2020 [PMID: 33045251] (WB, ChIP, Human) WB, ChIP Human
Aharonov A, Shakked A, Umansky KB, Savidor A ERBB2 drives YAP activation and EMT-like processes during cardiac regeneration bioRxiv Jan 1 2020 (IP, Mouse) IP Mouse
Lorenzo-Martin LF, Fernandez-Parejo N, Menacho-Marquez M et al. VAV2 signaling promotes regenerative proliferation in both cutaneous and head and neck squamous cell carcinoma Nat Commun Sep 22 2020 [PMID: 32963234] (IHC-P, Mouse) IHC-P Mouse
Chakravarti D, Hu B, Mao X et al. Telomere dysfunction activates YAP1 to drive tissue inflammation Nat Commun Sep 21 2020 [PMID: 32958778] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Yamashita A, Yoshitomi H, Kihara S et al. Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells Stem Cells Transl Med Aug 21 2020 [PMID: 32822104] (WB, KD, Human) WB, KD Human
Wilson KD, Ameen M, Guo H et al. Endogenous Retrovirus-Derived lncRNA BANCR Promotes Cardiomyocyte Migration in Humans and Non-human Primates Dev. Cell Jul 28 2020 [PMID: 32763147]
Zhang X, Liu P, Shang Y et al. Metformin and LW6 impairs pancreatic cancer cells and reduces nuclear localization of YAP1 J Cancer. Dec 6 2019 [PMID: 31897243] (WB, ICC/IF, Human, Mouse) WB, ICC/IF Human, Mouse
Show All 48 Publications.

Reviews for YAP1 Antibody (NB110-58358) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Zebrafish
.

Reviews using NB110-58358:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Zebrafish
(1)
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Images Ratings Applications Species Date Details
Western Blot YAP1 NB110-58358
Enlarge
3
reviewed by:
Anonymous
WB Human 02/04/2019
View

Summary

ApplicationWestern Blot
Sample TestedHuman prostate cancer cell line (PC3)
SpeciesHuman
Lot-
Western Blot YAP1 NB110-58358
Enlarge
4
reviewed by:
Anonymous
WB Zebrafish
 01/24/2018
View

Summary

ApplicationWestern Blot
Sample TestedProtein Lysate
SpeciesZebrafish
Lotna

Comments

CommentsLysate prepared with RIPA buffer. Reducing agent was used. 70 deg C for 10 min. Ran on 4-12% bis tris gel at 200v for 40 min.Primary antibody was used at a dilution of 1:1000 and incubated overnight at 4C. Secondary antibody was RDyde 800 CW Goat anti-Rabbit IgG diluted 1:5000 incubated overnight at 4C. Detection method was Licor Odyssey detection.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for YAP1 Antibody (NB110-58358). (Showing 1 - 2 of 2 FAQs).

  1. I am trying to find a good antibody for YAP1 to use in a ChIP. The one that has been published and seems to work in ChIP was produced by Marius Sudol's lab. Is the YAP antibody NB110-58358 the one Marius Sudol sent you or did you produce it using his antigen?
    • You are correct. This antibody is from Sudol's lab, and yes it was produced directly by him.
  2. Our customer purchased NB110-58358 for IHC-P. The sample would be mouse skin. Now they face the problem of choosing which protocol of antigen retrieval is the best one to work with NB110-58358, since there are several listed on http://www.novusbio.com/support/support-by-application/antigen-retrieval/protocol.html. Would you please help suggest?
    • To answer your customer's question, we do not have any protocol specific to NB110-58358 but as a general guideline we usually recommend using Citrate buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0) for antigen retrieving. 20 minutes incubation is usually sufficient in our experience.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-58358AF350
Alexa Fluor 532 NB110-58358AF532
Alexa Fluor 594 NB110-58358AF594
Alexa Fluor 750 NB110-58358AF750
DyLight 350 NB110-58358UV
DyLight 594 NB110-58358DL594
DyLight 680 NB110-58358FR
DyLight 755 NB110-58358IR
Janelia Fluor 549 NB110-58358JF549
Janelia Fluor 646 NB110-58358JF646

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Blogs on YAP1.

YAP1 - a transcription co-activator and the downstream target of Hippo pathway
YAP1 (Yes-associated protein 1) is a  transcriptional co-activator which acts as a major effector of Hippo signaling pathway that regulates organ size/ tissue homeostasis and cell proliferation, and is an established oncogene (1).  Hippo sig...  Read full blog post.

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Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
0

Anonymous
02/04/2019
Application: WB
Species: Human

Anonymous
01/24/2018
Application: WB
Species: Zebrafish

Bioinformatics

Gene Symbol YAP1