Genetic Strategies: Knockout Validated: YAP1 Antibody [NB110-58358] - Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A ...read more
Genetic Strategies: Knockout Validated: YAP1 Antibody [NB110-58358] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane ...read more
Immunocytochemistry/ Immunofluorescence: YAP1 Antibody [NB110-58358] - Analysis in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (red) and ...read more
Immunohistochemistry-Paraffin: YAP1 Antibody [NB110-58358] - YAP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human YAP1 polyclonal Antibody (Catalog # NB110-58358) at ...read more
Western Blot: YAP1 Antibody [NB110-58358] - Actomyosin activity inhibits beta-catenin- and YAP-driven proliferation of confluent keratinocytes. Effects of cell density and actomyosin activity on YAP phosphorylation. ...read more
Western Blot: YAP1 Antibody [NB110-58358] - Analysis in transfected HEK 293 cell lysate using YAP1 antibody. Observed molecular weight 75 kDa.
Western Blot: YAP1 Antibody [NB110-58358] - Consequences of ORP5 and ORP8 knockdown on downstream MAPK and PI3K/AKT signaling. Protein from MOH parental, single and double ORP knockdowns as well as cells transfected ...read more
Simple Western: YAP1 Antibody [NB110-58358] - Simple Western lane view shows a specific band for YAP1 in 0.1 mg/ml of HeLa lysate. Observed molecular weight is 75 kDa. This experiment was performed under reducing ...read more
Use in Immunoblotting reported in scientific literature (PMID: 28406163). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 28581498). Use in Knockdown Validated reported in scientific literature (PMID:28406163). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
48 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Drosophila reactivity reported in scientific literature (PMID: 29262338).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for YAP1 Antibody
65 kDa Yes-associated protein
Protein Yorkie Homolog
Transcriptional Coactivator YAP1
YAP65yes-associated protein 2
Yes Associated Protein
Yes-associated protein 1
Yes-associated protein 1, 65kDa
yes-associated protein beta
yes-associated protein delta
Yes-Associated Protein YAP65 Homolog
The transcriptional coactivator Yes-associated protein (YAP), also known as Yes-associated protein 1 (YAP1), Yes-Associated Protein YAP65 Homolog, Protein Yorkie Homolog and YAP65 has a theoretical molecular weight of 65 kDa. The YAP1 gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. YAP1 shares homology with the WW domain of transcriptional co-activator with PDZ-binding motif (TAZ), which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors and is likely involved in protein-protein interaction. YAP has been identified as a core regulatory mechanism that blocks mammalian glial cell proliferation and cellular reprogramming following damage (1).
YAP plays a role in the development and progression of multiple cancers as a transcriptional regulator of the Hippo signaling pathway. YAP1 encodes a nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis to play a pivotal role in controlling cell growth and organ size and has emerged as a key player in tumor suppression (2,3). Deregulation of the Hippo pathway causes tumor formation and malignancy, with YAP being a key oncogenic driver in liver carcinogenesis (2) and may function as a potential target for cancer treatment (3).
1. Rueda, E. M., Hall, B. M., Hill, M. C., Swinton, P. G., Tong, X., Martin, J. F., & Poche, R. A. (2019). The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming. Cell Rep, 27(6), 1637-1649.e1636. doi:10.1016/j.celrep.2019.04.047
2. Liu, A. M., Xu, M. Z., Chen, J., Poon, R. T., & Luk, J. M. (2010). Targeting YAP and Hippo signaling pathway in liver cancer. Expert Opin Ther Targets, 14(8), 855-868. doi:10.1517/14728222.2010.499361
3.Ye, S., & Eisinger-Mathason, T. S. (2016). Targeting the Hippo pathway: Clinical implications and therapeutics. Pharmacol Res, 103, 270-278. doi:10.1016/j.phrs.2015.11.025
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Lysate prepared with RIPA buffer. Reducing agent was used. 70 deg C for 10 min. Ran on 4-12% bis tris gel at 200v for 40 min.Primary antibody was used at a dilution of 1:1000 and incubated overnight at 4C. Secondary antibody was RDyde 800 CW Goat anti-Rabbit IgG diluted 1:5000 incubated overnight at 4C. Detection method was Licor Odyssey detection.
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for YAP1 Antibody (NB110-58358). (Showing 1 - 2 of 2 FAQs).
I am trying to find a good antibody for YAP1 to use in a ChIP. The one that has been published and seems to work in ChIP was produced by Marius Sudol's lab. Is the YAP antibody NB110-58358 the one Marius Sudol sent you or did you produce it using his antigen?
You are correct. This antibody is from Sudol's lab, and yes it was produced directly by him.
Our customer purchased NB110-58358 for IHC-P. The sample would be mouse skin. Now they face the problem of choosing which protocol of antigen retrieval is the best one to work with NB110-58358, since there are several listed on http://www.novusbio.com/support/support-by-application/antigen-retrieval/protocol.html. Would you please help suggest?
To answer your customer's question, we do not have any protocol specific to NB110-58358 but as a general guideline we usually recommend using Citrate buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0) for antigen retrieving. 20 minutes incubation is usually sufficient in our experience.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.