TACE/ADAM17 Antibody - BSA Free

Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TACE KO celllysates. Antibodies: TACE/ADAM17 NBP1-77044 (0.25 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - HeLa whole cell lysate. Image from verified customer review.

Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - Analysis of TACE in HeLa (A, D), Jurkat (B, E) and Raji (C,F) cell lysate in (A-C) the absence or (D-F) the presence of blocking peptide with TACE antibody at 0.5 ug/mL.

Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - IAV via Protein Expression Profile in Cells Lines. Antibodies: TACE and GAPDH. Secondary: Goat anti-rabbit IgG HRP conjugate.

Immunocytochemistry/ Immunofluorescence: TACE/ADAM17 Antibody - BSA Free [NBP1-77044] - Figure 5 Immunocytochemistry Validation of TACE/ADAM17 in HeLa CellsImmunohistochemical analysis of HeLa cells using anti-TACE/ADAM17 antibody at 10 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: TACE/ADAM17 Antibody - BSA Free [NBP1-77044] - Figure 4 Immunofluorescence Validation of TACE/ADAM17 in HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TACE/ADAM17 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).

• Reactivity: Hu, Rt, Mu
• Applications: WB, Simple Western, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TACE/ADAM17 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TACE. The immunogen is located within the last 50 amino acids of TACE. Amino Acid Squence: ASFKLQRQNRVDSKETE
• Specificity: 80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE/ADAM17. Human TACE/ADAM17 has 2 isoforms, including isoform A (824aa, 93kD), isoform B (694aa, 78.5kD). This antibody detects only isoform A. Mouse TACE/ADAM17 has 2 isoforms, long isoform (827aa, 93.1kD) and short isoform (655aa, 73.9kD). Rat TACE/ADAM17 has only one isoform identified so far (827aa, 93kD).
• Predicted Species: Mouse (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADAM17
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Simple Western
  • Western Blot 0.25-2 ug/mL
• Theoretical MW: 93 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  TACE/ADAM17 Peptide (NBP1-77044PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TACE/ADAM17 Antibody - BSA Free

• ADAM 17
• ADAM metallopeptidase domain 17
• ADAM metallopeptidase domain 18
• ADAM17
• ADAM18
• CD156b antigen
• CD156b
• CSVP
• disintegrin and metalloproteinase domain-containing protein 17
• EC 3.4.24.86
• MGC71942
• Snake venom-like protease
• TACE
• TACEcSVP
• TNF-alpha convertase
• TNF-alpha converting enzyme
• TNF-alpha-converting enzyme
• tumor necrosis factor, alpha, converting enzyme

Background

Tumor-necrosis factor-alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-alpha is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-alpha. TNF-alpha converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TACE/ADAM17 Antibody (NBP1-77044)(2)

Publications using NBP1-77044

• Brishti MA, Raghavan S, Lamar K et al. Diabetic Endothelial Cell Glycogen Synthase Kinase 3? Activation Induces VCAM1 Ectodomain Shedding Int J Mol Sci 2023-09-14 [PMID: 37762417]
• Lemjabbar H, Li D, Gallup M et al. Tobacco smoke-induced lung cell proliferation mediated by tumor necrosis factor alpha-converting enzyme and amphiregulin. J Biol Chem. 2003-07-11 [PMID: 12711607]

Review for TACE/ADAM17 Antibody (NBP1-77044) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 09/09/2014

Summary

• Application: Western Blot
• Sample Tested: Hello cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: ADAM17
• Uniprot:
  • NP_003174(Human)