ADAM9 Antibody [Unconjugated]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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ADAM9 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ADAM9
Ala206-Asp697
Accession # Q61072
Specificity
Detects mouse ADAM9 Ectodomain in direct ELISAs and Western blots. In Western blots no cross-reactivity with the Ectodomain of recombinant mouse ADAM10 and rhADAM8, 15, and 17 (TACE) is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ADAM9
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • CyTOF-ready
  • Knockout Validated
Publications
Read Publications using
AF949 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM9 Antibody [Unconjugated]

  • a disintegrin and metalloproteinase domain 9 (meltrin gamma)
  • ADAM 9
  • ADAM metallopeptidase domain 9 (meltrin gamma)
  • ADAM metallopeptidase domain 9
  • ADAM9
  • Cellular disintegrin-related protein
  • cone rod dystrophy 9
  • CORD9
  • disintegrin and metalloproteinase domain-containing protein 9
  • EC 3.4.24
  • EC 3.4.24.-
  • MCMP
  • MCMPMDC9KIAA0021Mltng
  • MDC9
  • Meltrin gamma
  • Meltrin-gamma
  • Metalloprotease/disintegrin/cysteine-rich protein 9
  • MLTNG
  • Myeloma cell metalloproteinase

Background

ADAM9, also known as MDC9 or meltrin gamma, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1). Like other membrane‑anchored ADAMs, ADAM9 consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. ADAM9 is able to cleave peptides corresponding to cleavage sites of tumor necrosis factor-alpha (TNF-alpha ), the p75-TNF receptor, the beta -amyloid protein precursor, and the c-kit ligand-1, implying that it may participate in shedding of these membrane proteins (2). In fact, ADAM9 has been shown to shed membrane‑anchored heparin‑binding EGF-like growth factor (3). In addition, it also cleaves oxidized insulin B-chain and fibronectin (2, 4). Besides its catalytic activity, ADAM9 functions as an adhesion molelcule through binding of its disintegrin domain to integrins such as alpha v beta 5 and alpha 6 beta 1 (5, 6). The cytoplasmic domain of ADAM9 interacts with Src homology 3
(SH3)‑containing proteins and protein kinase C, and may mediate different signaling pathways (3, 7). ADAM9 is widely expressed in tissues (8).

  1. Moss, M.L. et al. (2001) Drug Discov. Today 6:417.
  2. Roghani, M. et al. (1999) J. Biol. Chem. 274:3531.
  3. Izumi, Y. et al. (1998) EMBO J. 17:7260.
  4. Schwettmann, L. and H. Tschesche (2001) Protein. Expr. Purif. 21:65. 
  5. Nath, D. et al. (2000) J. Cell Sci. 113:2319.
  6. Zhou, M. et al. (2001) Biochem. Biophys. Res. Comm. 280:574.
  7. Howard, L. et al. (1999) J. Biol. Chem. 274:31693.
  8. Weskamp, G. et al. (1996) J. Cell Biol. 132:717.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ADAM9 Antibody (AF949)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, Neutralization, Western Blot.


Filter By Application
IHC
(2)
Neutralization
(1)
Western Blot
(5)
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Human
(4)
Mouse
(4)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF949 Applications Species
LE Bazzone, M King, CR MacKay, PP Kyawe, P Meraner, D Lindstrom, J Rojas-Quin, CA Owen, JP Wang, AL Brass, EA Kurt-Jones, RW Finberg A Disintegrin and Metalloproteinase 9 Domain (ADAM9) Is a Major Susceptibility Factor in the Early Stages of Encephalomyocarditis Virus Infection MBio, 2019;10(1):. 2019 [PMID: 30723129] (Western Blot, Human) Western Blot Human
KJ Mygind, T Störiko, ML Freiberg, J Samsøe-Pet, J Schwarz, OM Andersen, M Kveiborg Sorting nexin 9 (SNX9) regulates levels of the transmembrane disintegrin and metalloproteinase (ADAM)-9 at the cell surface J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29622675] (Western Blot, Human) Western Blot Human
CY Lin, CF Cho, ST Bai, JP Liu, TT Kuo, LJ Wang, YS Lin, CC Lin, LC Lai, TP Lu, CY Hsieh, CN Chu, DC Cheng, YP Sher ADAM9 promotes lung cancer progression through vascular remodeling by VEGFA, ANGPT2, and PLAT Sci Rep, 2017;7(1):15108. 2017 [PMID: 29118335] (Neutralization, Human) Neutralization Human
N Giebeler, A Schönefu beta, J Landsberg, T Tüting, C Mauch, P Zigrino Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasis Oncogene, 2017;0(0):. 2017 [PMID: 28553955] (IHC, Mouse) IHC Mouse
Wing Yip Tam The association between laminin and microglial morphology in vitro Sci Rep, 2016;6(0):28580. 2016 [PMID: 27334934] (IHC, Mouse) IHC Mouse
Delarasse C, Auger R, Gonnord P, Fontaine B, Kanellopoulos JM The purinergic receptor P2X7 triggers alpha-secretase-dependent processing of the amyloid precursor protein. J. Biol. Chem., 2011;286(4):2596-606. 2011 [PMID: 21081501] (Western Blot, Mouse) Western Blot Mouse
Mauch C, Zamek J, Abety AN, Grimberg G Accelerated wound repair in ADAM-9 knockout animals. J. Invest. Dermatol., 2010;130(8):2120-30. 2010 [PMID: 20376065] (Western Blot, Mouse) Western Blot Mouse
Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3. J. Cell Biol., 2006;175(1):179-91. 2006 [PMID: 17030988] (Western Blot, Human) Western Blot Human

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF949

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Bioinformatics

Gene Symbol ADAM9
Uniprot