Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ADAM9 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat ...read more
Western blot shows lysates of C2C12 mouse myoblast cell line and WI‑38 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ADAM9 Ectodomain Antigen ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for ADAM9 Antibody [Unconjugated]
a disintegrin and metalloproteinase domain 9 (meltrin gamma)
ADAM metallopeptidase domain 9 (meltrin gamma)
ADAM metallopeptidase domain 9
Cellular disintegrin-related protein
cone rod dystrophy 9
disintegrin and metalloproteinase domain-containing protein 9
Metalloprotease/disintegrin/cysteine-rich protein 9
Myeloma cell metalloproteinase
ADAM9, also known as MDC9 or meltrin gamma , is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1). Like other membrane‑anchored ADAMs, ADAM9 consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. ADAM9 is able to cleave peptides corresponding to cleavage sites of tumor necrosis factor-alpha (TNF-alpha ), the p75-TNF receptor, the beta -amyloid protein precursor, and the c-kit ligand-1, implying that it may participate in shedding of these membrane proteins (2). In fact, ADAM9 has been shown to shed membrane‑anchored heparin‑binding EGF-like growth factor (3). In addition, it also cleaves oxidized insulin B-chain and fibronectin (2, 4). Besides its catalytic activity, ADAM9 functions as an adhesion molelcule through binding of its disintegrin domain to integrins such as alpha v beta 5 and alpha 6 beta 1 (5, 6). The cytoplasmic domain of ADAM9 interacts with Src homology 3 (SH3)‑containing proteins and protein kinase C, and may mediate different signaling pathways (3, 7). ADAM9 is widely expressed in tissues (8).
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Roghani, M. et al. (1999) J. Biol. Chem. 274:3531.
Izumi, Y. et al. (1998) EMBO J. 17:7260.
Schwettmann, L. and H. Tschesche (2001) Protein. Expr. Purif. 21:65.
Nath, D. et al. (2000) J. Cell Sci. 113:2319.
Zhou, M. et al. (2001) Biochem. Biophys. Res. Comm. 280:574.
Howard, L. et al. (1999) J. Biol. Chem. 274:31693.
Weskamp, G. et al. (1996) J. Cell Biol. 132:717.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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