ADAM9 Antibody [Unconjugated]


Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ADAM9 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat more
Western blot shows lysates of C2C12 mouse myoblast cell line and WI‑38 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ADAM9 Ectodomain Antigen more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready, KO
Validated by:

Genetic Strategies


Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

ADAM9 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse ADAM9
Accession # Q61072
Detects mouse ADAM9 Ectodomain in direct ELISAs and Western blots. In Western blots no cross-reactivity with the Ectodomain of recombinant mouse ADAM10 and rhADAM8, 15, and 17 (TACE) is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Knockout Validated
  • Western Blot 1 ug/mL
Read Publications using
AF949 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM9 Antibody [Unconjugated]

  • a disintegrin and metalloproteinase domain 9 (meltrin gamma)
  • ADAM 9
  • ADAM metallopeptidase domain 9 (meltrin gamma)
  • ADAM metallopeptidase domain 9
  • ADAM9
  • Cellular disintegrin-related protein
  • cone rod dystrophy 9
  • CORD9
  • disintegrin and metalloproteinase domain-containing protein 9
  • EC 3.4.24
  • EC 3.4.24.-
  • MCMP
  • MCMPMDC9KIAA0021Mltng
  • MDC9
  • Meltrin gamma
  • Meltrin-gamma
  • Metalloprotease/disintegrin/cysteine-rich protein 9
  • Myeloma cell metalloproteinase


ADAM9, also known as MDC9 or meltrin gamma , is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1). Like other membrane‑anchored ADAMs, ADAM9 consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. ADAM9 is able to cleave peptides corresponding to cleavage sites of tumor necrosis factor-alpha (TNF-alpha ), the p75-TNF receptor, the beta -amyloid protein precursor, and the c-kit ligand-1, implying that it may participate in shedding of these membrane proteins (2). In fact, ADAM9 has been shown to shed membrane‑anchored heparin‑binding EGF-like growth factor (3). In addition, it also cleaves oxidized insulin B-chain and fibronectin (2, 4). Besides its catalytic activity, ADAM9 functions as an adhesion molelcule through binding of its disintegrin domain to integrins such as alpha v beta 5 and alpha 6 beta 1 (5, 6). The cytoplasmic domain of ADAM9 interacts with Src homology 3
(SH3)‑containing proteins and protein kinase C, and may mediate different signaling pathways (3, 7). ADAM9 is widely expressed in tissues (8).

  1. Moss, M.L. et al. (2001) Drug Discov. Today 6:417.
  2. Roghani, M. et al. (1999) J. Biol. Chem. 274:3531.
  3. Izumi, Y. et al. (1998) EMBO J. 17:7260.
  4. Schwettmann, L. and H. Tschesche (2001) Protein. Expr. Purif. 21:65. 
  5. Nath, D. et al. (2000) J. Cell Sci. 113:2319.
  6. Zhou, M. et al. (2001) Biochem. Biophys. Res. Comm. 280:574.
  7. Howard, L. et al. (1999) J. Biol. Chem. 274:31693.
  8. Weskamp, G. et al. (1996) J. Cell Biol. 132:717.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: EnzAct
Species: Hu
Applications: EnzAct
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: CyTOF-ready, ICC, IHC, IP, ICFlow, WB
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
Species: Hu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu, Mu
Applications: CyTOF-ready, IHC, ICFlow, WB
Species: Hu
Applications: ICC, IP, WB
Species: Hu
Applications: BA
Species: Hu, Mu
Applications: ICC/IF, IHC, WB
Species: Av, Bv, Sh
Applications: WB
Species: Hu
Applications: BA
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
Species: Hu
Applications: IHC
Species: Hu, Mu
Applications: WB, Flow, IHC, IP, CyTOF-ready, KO

Publications for ADAM9 Antibody (AF949)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, Neutralization, Western Blot.

Filter By Application
Western Blot
All Applications
Filter By Species
All Species
Showing Publications 1 - 9 of 9.
Publications using AF949 Applications Species
WC Cheng, CY Chang, CC Lo, CY Hsieh, TT Kuo, GC Tseng, SC Wong, SF Chiang, KC Huang, LC Lai, TP Lu, KSC Chao, YP Sher Identification of theranostic factors for patients developing metastasis after surgery for early-stage lung adenocarcinoma Theranostics, 2021;11(8):3661-3675. 2021 [PMID: 33664854] (IHC, Human) IHC Human
LE Bazzone, M King, CR MacKay, PP Kyawe, P Meraner, D Lindstrom, J Rojas-Quin, CA Owen, JP Wang, AL Brass, EA Kurt-Jones, RW Finberg A Disintegrin and Metalloproteinase 9 Domain (ADAM9) Is a Major Susceptibility Factor in the Early Stages of Encephalomyocarditis Virus Infection MBio, 2019;10(1):. 2019 [PMID: 30723129] (Western Blot, Human) Western Blot Human
KJ Mygind, T Störiko, ML Freiberg, J Samsøe-Pet, J Schwarz, OM Andersen, M Kveiborg Sorting nexin 9 (SNX9) regulates levels of the transmembrane disintegrin and metalloproteinase (ADAM)-9 at the cell surface J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29622675] (Western Blot, Human) Western Blot Human
CY Lin, CF Cho, ST Bai, JP Liu, TT Kuo, LJ Wang, YS Lin, CC Lin, LC Lai, TP Lu, CY Hsieh, CN Chu, DC Cheng, YP Sher ADAM9 promotes lung cancer progression through vascular remodeling by VEGFA, ANGPT2, and PLAT Sci Rep, 2017;7(1):15108. 2017 [PMID: 29118335] (Neutralization, Human) Neutralization Human
N Giebeler, A Schönefu beta , J Landsberg, T Tüting, C Mauch, P Zigrino Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasis Oncogene, 2017;0(0):. 2017 [PMID: 28553955] (IHC, Mouse) IHC Mouse
Wing Yip Tam The association between laminin and microglial morphology in vitro Sci Rep, 2016;6(0):28580. 2016 [PMID: 27334934] (IHC, Mouse) IHC Mouse
Delarasse C, Auger R, Gonnord P, Fontaine B, Kanellopoulos JM The purinergic receptor P2X7 triggers alpha-secretase-dependent processing of the amyloid precursor protein. J. Biol. Chem., 2011;286(4):2596-606. 2011 [PMID: 21081501] (Western Blot, Mouse) Western Blot Mouse
Mauch C, Zamek J, Abety AN, Grimberg G Accelerated wound repair in ADAM-9 knockout animals. J. Invest. Dermatol., 2010;130(8):2120-30. 2010 [PMID: 20376065] (Western Blot, Mouse) Western Blot Mouse
Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3. J. Cell Biol., 2006;175(1):179-91. 2006 [PMID: 17030988] (Western Blot, Human) Western Blot Human

Reviews for ADAM9 Antibody (AF949) (0)

There are no reviews for ADAM9 Antibody (AF949). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ADAM9 Antibody (AF949) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional ADAM9 Products

Bioinformatics Tool for ADAM9 Antibody (AF949)

Discover related pathways, diseases and genes to ADAM9 Antibody (AF949). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ADAM9 Antibody (AF949)

Discover more about diseases related to ADAM9 Antibody (AF949).

Pathways for ADAM9 Antibody (AF949)

View related products by pathway.

PTMs for ADAM9 Antibody (AF949)

Learn more about PTMs related to ADAM9 Antibody (AF949).

Blogs on ADAM9

There are no specific blogs for ADAM9, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our ADAM9 Antibody [Unconjugated] and receive a gift card or discount.


Gene Symbol ADAM9