Synaptophysin Antibody [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Synaptophysin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Synaptophysin
Glu50-Phe106
Accession # P08247
Specificity
Detects human Synaptophysin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SYP
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF5555 in the following applications:

Publications
Read Publications using
AF5555 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Synaptophysin Antibody [Unconjugated]

  • Major synaptic vesicle protein p38
  • MRX
  • MRXSYP
  • Synaptophysin
  • SYP

Background

Synaptophysin (also p38 and BM89 in mouse) is a 38‑40 kDa member of the synaptophysin/synaptobrevin family of proteins. It is expressed in neurons, and interacts with VAMP2 at an early stage of vesicle formation, guiding VAMP2 placement into secretory vesicles that are destined for release upon stimulation. Human Synaptophysin is a four transmembrane protein that is 313 amino acids (aa) in length. It contains two luminal domains (aa 50‑106 and 162‑199) and a cytoplasmic C‑terminal targeting region that specifies its localization (aa 214‑313). Synaptophysin forms homodimers and oligomers, and heterodimerizes with VAMP2. There is one isoform variant that shows a six aa substitution for aa 35‑313. Over aa 50‑106, human Synaptophysin shares 89% aa identity with mouse Synaptophysin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Synaptophysin Antibody (AF5555)(3)

Review for Synaptophysin Antibody (AF5555) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF5555:
Filter by Applications
IF
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Synaptophysin AF5555
Enlarge
5
reviewed by:
Anonymous
IF Mouse 05/18/2016
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Summary

ApplicationImmunofluorescence
Sample Testedmouse brain
SpeciesMouse

Blocking

Blocking Details10% donkey serum in 0.1 M soidum phosphate, pH 7.4 for 30 min at RT

Primary Anitbody

Dilution Ratio3 ug/ml, 48 hrs, 4 degrees C (refrigerator), 0.1 M sodium phosphate, pH 7.4,.05% Triton

Secondary Antibody

Secondary DescriptionDonkey anti-goat Alexa Fluor 555
Secondary Manufacturer Cat#A21432 life technologies
Secondary Concentration1:1500 in 0.1.M sodium phosphate, pH 7.4 (no Triton)

Details

Detection NotesNormal serum
Fixation DetailsTissue was fixed by cardiac perfusion with 4% paraformaldehyde in 0.1 M sodium phosphate and fixed overnight in the same fix.
Wash Description0.05% Triton in 0.1 M sodium phosphate X 3

Comments

CommentsThe antibody labels very well at this concentration and will probably label at lower concentrations.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Synaptophysin Antibody (AF5555) (0)

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Secondary Antibodies

 

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Blogs on Synaptophysin.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.

Synaptophysin a Marker Protein in Neuroendocrine Cells
Synaptophysin a Marker Protein in Neuroendocrine Cells Synaptophysin is a major integral membrane glycoprotein of neuronal synaptic vesicles present in virtually all synapses and shows a high degree of evolutionary conservation across the mammals. Syn...  Read full blog post.

Characterizing Synaptophysin is "a Snap"
Synaptophysin is an integral membrane glycoprotein found within the small synaptic vesicles in brain and endocrine cells. Studies with synaptophysin antibodies show that it is one of the most abundant small vesicle proteins, constituting approximately...  Read full blog post.

Synaptophysin and Dementing Disorders
 Synaptophysin (a presynaptic vesicle protein) is an integral membrane glycoprotein originally isolated from presynaptic vesicles of bovine neurons. Synaptophysin is found in all nerve terminals and synaptophysin measurements have been used to quantif...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
05/18/2016
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol SYP
Uniprot