SUV39H2 Antibody

Western Blot: SUV39H2 Antibody [NB100-1140] - staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry: SUV39H2 Antibody [NB100-1140] - Dermal Microvascular Endothelial Cells were fixed with 4% Paraformaldehyde followed by immunofluorescence staining using 1:100 dilution of pAb. This image was submitted via customer Review.

• Reactivity: Hu, Rt, Mu, Po, Bv, Ca
• Applications: WB, ELISA, ICC/IF, IP, ChIP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SUV39H2 Antibody Summary

• Immunogen: Peptide with sequence CKCGAVTCRGYLN corresponding to C-Terminus according to NP_078946.1.
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SUV39H2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Peptide ELISA detection limit 1:8000
  • Western Blot 1-3 ug/ml
• Application Notes: ChIP: An anonymous customer has reported it to work very well. IP per custumer review. ICC per customer review. WB: Approx. 38 kDa band observed in rat kidney and testis lysates (calculated MW of 39.9 kDa band according to NP_078946.1).Preliminary testing was unsuccessful on Mouse, Human and Pig Testis and HEK293 cell line for this particular batch. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:31682228).
• Control:

  SUV39H2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SUV39H2 Antibody

• FLJ23414Histone H3-K9 methyltransferase 2
• H3-K9-HMTase 2
• histone-lysine N-methyltransferase SUV39H2
• KMT1BEC 2.1.1.43
• Lysine N-methyltransferase 1B
• Su(var)3-9 homolog 2
• suppressor of variegation 3-9 homolog 2 (Drosophila)
• Suppressor of variegation 3-9 homolog 2

Background

Suppressor of variegation 3-9 homolog 2 (Drosophila) is a histone lysine N-methyltransferase activity (H3-K9 specific). This protein contains a SET domain (HMTase activity) and binds chromatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SUV39H2 Antibody (NB100-1140)(2)

Publications using NB100-1140

• Roy A, Ghosh A, Kumar B, Chandran B IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP Elife 2019-11-04 [PMID: 31682228] (IP, ICC/IF, Chemotaxis, WB, Human)
• Peters AH, O'Carroll D, Scherthan H et al. Loss of the Suv39h histone methyltransferases impairs mammalian heterochromatin and genome stability. Cell 2001-11-02 [PMID: 11701123]

Reviews for SUV39H2 Antibody (NB100-1140) (3)

Average Rating: 3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Verified Customer IP Human 05/16/2018

Summary

• Application: Immunoprecipitation
• Sample Tested: RL human non-Hodgkin's lymphoma B cell line
• Species: Human
• Lot: P1E280211

reviewed by: Verified Customer ICC Human 07/11/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Human Dermal Microvascular Endothelial cells - fixed cells used in In-cell ELISA,Human Dermal Microvascular Endothelial Cells - Paraformaldehyde Fixed
• Species: Human
• Lot: P1E280211

reviewed by: Arunava Roy WB Human 05/10/2017

Summary

• Application: Western Blot
• Sample Tested: Human Dermal Microvascular Endothelial whole cell lysate
• Species: Human
• Lot: P1E280211

Bioinformatics

• Gene Symbol: SUV39H2
• Entrez:
  • 79723(Human)
• Uniprot:
  • NP_078946.1(Human)