PCAF Antibody (5E11)

Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in A-431 (Cat # L015V1).

Immunocytochemistry/Immunofluorescence: PCAF Antibody (5E11) [H00008850-M04] - Analysis of monoclonal antibody to PCAF on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in LNCaP (Cat # L004V1).

Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in Hela S3 NE (Cat # L013V3).

Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in human uterus myoma.

Sandwich ELISA: PCAF Antibody (5E11) [H00008850-M04] - Detection limit for recombinant GST tagged PCAF is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 5E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PCAF Antibody (5E11) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PCAF (AAH60823, 353 a.a. ~ 452 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LEEEVYSQNSPIWDQDFLSASSRTSQLGIQTVINPPPVAGTISYNSTSSSLEQPNAGSSSPACKASSGLEANPGEKRKMTDSHVLEEAKKPRVMGDIPME
• Specificity: PCAF (5E11)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KAT2B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against tissue lysate, cell lysate and recombinant protein for WB. It has been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PCAF Antibody (5E11)

• CREBBP-associated factor
• EC 2.3.1.48
• GCN5
• GCN5L
• Histone acetylase PCAF
• histone acetyltransferase KAT2B
• Histone acetyltransferase PCAF
• K(lysine) acetyltransferase 2B
• KAT2B
• Lysine acetyltransferase 2B
• P
• P/CAFGCN5
• P300/CBP-associated factorCAF
• PCAF

Background

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for PCAF Antibody (H00008850-M04) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 12/22/2014

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate from HEK 293T cells
• Species: Human

Bioinformatics

• Gene Symbol: KAT2B
• Entrez:
  • 8850(Human)
• OMIM:
  • 602303(Human)
• Uniprot:
  • AAH60823(Human)