SMYD3 Antibody - BSA Free

Western Blot: SMYD3 Antibody [NBP2-20430] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: SMYD3 Antibody [NBP2-20430] - HCT116 cells were fixed in ice-cold MeOH for 5 min. Green: SMYD3 protein stained by SMYD3 antibody diluted at 1:500.Blue: Hoechst 33342 staining.

Chromatin Immunoprecipitation: SMYD3 Antibody [NBP2-20430] - Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either normal rabbit IgG or anti-SMYD3 antibody. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8..

Immunoprecipitation: SMYD3 Antibody [NBP2-20430] - Immunoprecipitation of SMYD3 protein from 293T whole cell extracts using 5 ug of SMYD3 antibody. Western blot analysis was performed using SMYD3 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

SMYD3 Antibody [NBP2-20430] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: SMYD3 Antibody [NBP2-20430] - SMYD3 antibody detects SMYD3 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (NBP2-20430) diluted at a dilution of 1:1000.

Western Blot: SMYD3 Antibody [NBP2-20430] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (NBP2-20430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Po, Pm
• Applications: WB, ICC/IF, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SMYD3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SMYD3 Antibody - BSA Free (NBP2-20430) is a polyclonal antibody validated for use in WB, ICC/IF, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SMYD3. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus
• Predicted Species: Porcine (92%), Monkey (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMYD3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (99%), Bovine (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SMYD3 Antibody - BSA Free

• bA74P14.1 (novel protein)
• bA74P14.1
• EC 2.1.1
• EC 2.1.1.43
• FLJ21080
• KMT3E
• MGC104324
• MYND domain containing 1
• SET and MYND domain containing 3
• SET and MYND domain-containing protein 3
• SMYD3
• Zinc finger MYND domain-containing protein 1
• zinc finger protein, subfamily 3A (MYND domain containing), 1
• ZMYND1
• ZNFN3A1

Background

SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMYD3