Fucosyltransferase 3/FUT3 Antibody (2-25LE) - BSA Free

Immunohistochemistry: Blood Group Lewis b Antibody (2-25LE) [NB500-526] - Immunohistochemistry staining of human colon adenocarcinoma (paraffin sections) using anti-blood group Lewis b (clone 2-25LE).

Immunohistochemistry: Blood Group Lewis b Antibody (2-25LE) [NB500-526] - Staining of human small intestine (paraffin sections) using anti-blood group Lewis b (clone 2-25LE).

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 2-25LE
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Fucosyltransferase 3/FUT3 Antibody (2-25LE) - BSA Free Summary

• Immunogen: Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).
• Specificity: The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FUT3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Western Blot 1:1000
• Application Notes: Purity is > 95% (by SDS-PAGE). Heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Fucosyltransferase 3/FUT3 Antibody (2-25LE) - BSA Free

• CD174
• FT3B
• Fucosyltransferase 3
• FucT-III
• FUT3
• LE
• Les
• Lewis FT

Background

The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fucosyltransferase 3/FUT3 Antibody (NB500-526)(3)

Publications using NB500-526

• Montesino B, Steenackers A, Lozano JM et al. Identification of alpha 1,2-Fucosylated Signaling and adhesion molecules in head and neck squamous cell carcinoma Glycobiology 2021-12-22 [PMID: 34939118]
• Creuzot-Garcher C, Guerzider V, Assem M et al. Alteration of sialyl Lewis epitope expression in pterygium. Invest Ophthalmol Vis Sci. 1999-07-01 [PMID: 10393028]
• First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems. Rev Fr Transfus Immunohematol. 1988-04-01 [PMID: 3212306]

Bioinformatics

• Gene Symbol: FUT3
• Entrez:
  • 2525(Human)
• Uniprot:
  • P21217()