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Fucosyltransferase 3/FUT3 Antibody (2-25LE)

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Immunohistochemistry: Blood Group Lewis b Antibody (2-25LE) [NB500-526] - Immunohistochemistry staining of human colon adenocarcinoma (paraffin sections) using anti-blood group Lewis b (clone 2-25LE).
Immunohistochemistry: Blood Group Lewis b Antibody (2-25LE) [NB500-526] - Immunohistochemistry staining of human colon adenocarcinoma (paraffin sections) using anti-blood group Lewis b (clone 2-25LE).
Immunohistochemistry: Blood Group Lewis b Antibody (2-25LE) [NB500-526] - Staining of human small intestine (paraffin sections) using anti-blood group Lewis b (clone 2-25LE).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
2-25LE
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB500-526AF350
Alexa Fluor 405
NB500-526AF405
Alexa Fluor 488
NB500-526AF488
Alexa Fluor 532
NB500-526AF532
Alexa Fluor 594
NB500-526AF594
Alexa Fluor 647
NB500-526AF647
Alexa Fluor 700
NB500-526AF700
Alexa Fluor 750
NB500-526AF750
Biotin
NB500-526B
DyLight 350
NB500-526UV
DyLight 405
NB500-526V
DyLight 488
NB500-526G
DyLight 550
NB500-526R
DyLight 594
NB500-526DL594
DyLight 650
NB500-526C
DyLight 680
NB500-526FR
DyLight 755
NB500-526IR
FITC
NB500-526F
HRP
NB500-526H
Janelia Fluor 549
NB500-526JF549
Janelia Fluor 646
NB500-526JF646
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Fucosyltransferase 3/FUT3 Antibody (2-25LE) Summary

Immunogen
Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).
Specificity
The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FUT3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:2000
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
Application Notes
Purity is > 95% (by SDS-PAGE). Heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB
Publications
Read Publications using NB500-526.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Fucosyltransferase 3/FUT3 Antibody (2-25LE)

  • CD174
  • FT3B
  • Fucosyltransferase 3
  • FucT-III
  • FUT3
  • Les
  • Lewis FT

Background

The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Fucosyltransferase 3/FUT3 Antibody (2-25LE)
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Publications for Blood Group Lewis b Antibody (NB500-526)(2)


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FAQs for Blood Group Lewis b Antibody (NB500-526) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB500-526AF350
Alexa Fluor 405 NB500-526AF405
Alexa Fluor 488 NB500-526AF488
Alexa Fluor 532 NB500-526AF532
Alexa Fluor 594 NB500-526AF594
Alexa Fluor 647 NB500-526AF647
Alexa Fluor 700 NB500-526AF700
Alexa Fluor 750 NB500-526AF750
Biotin NB500-526B
DyLight 350 NB500-526UV
DyLight 405 NB500-526V
DyLight 488 NB500-526G
DyLight 550 NB500-526R
DyLight 594 NB500-526DL594
DyLight 650 NB500-526C
DyLight 680 NB500-526FR
DyLight 755 NB500-526IR
FITC NB500-526F
HRP NB500-526H
Janelia Fluor 549 NB500-526JF549
Janelia Fluor 646 NB500-526JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol FUT3
Entrez
Uniprot