Sulfatase-2/SULF2 Antibody (2B4) [Biotin]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
2B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

Sulfatase-2/SULF2 Antibody (2B4) [Biotin] Summary

Immunogen
Partial recombinant human Sulfatase 2 isoform b [Accession# NP_940998].
Localization
Endoplasmic reticulum
Specificity
Does not recognize human or mouse Sulfatase 1 or other family members.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SULF2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Sulfatase 2 (2B4) antibody is useful for Western Blot, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and ELISA. For WB a band can be seen at 50 kDa. Other bands may be seen at 135 kDa (unprocessed protein) and 37 kDa (fragment of 50 kDa subunit). For ELISA, use this antibody at 1-10 ug/ml for capture and 1-5 ug/ml for detection. It has been reported that NBP1-36727 picks up a 130 kDa band that is not Sulfatase 2 in human plasma. Because of this we do not recommend NBP1-36727 for capture of the human protein.
Readout System

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Sulfatase-2/SULF2 Antibody (2B4) [Biotin]

  • DKFZp313E091
  • EC 3.1.6
  • EC 3.1.6.-
  • EC 3.1.6.14
  • extracellular sulfatase Sulf-2
  • FLJ44488
  • FLJ90554
  • hSulf-2
  • KIAA1247HSULF-2
  • MGC126411
  • SULF2
  • SULF-2
  • sulfatase 2
  • Sulfatase2
  • Sulfatase-2

Background

Heparan sulfate 6-O-endosulfatases, such as Sulfatase 2, selectively remove 6-O-sulfate groups from heparan sulfate. Heparan sulfate proteoglycans (HSPGs) act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules (Dai et al., 2005 [PubMed 16192265]) [supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NBP1-36727V
DyLight 488 Labeled NBP1-36727G
DyLight 550 Labeled NBP1-36727R
DyLight 650 Labeled NBP1-36727C
FITC Labeled NBP1-36727F
HRP Labeled NBP1-36727H

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Bioinformatics Tool for Sulfatase-2/SULF2 Antibody (NBP1-36727B)

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Bioinformatics

Gene Symbol SULF2
Entrez