Sulfatase-2/SULF2 Antibody (2B4)

Images

 
Western Blot: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Analysis showing Sulfatase 2 expression in MCF7 conditioned media (CM). In CM, the 75 kDa band is dominant (Tang R, Rosen SD. J Biol Chem. 2009 Aug ...read more
Immunohistochemistry-Paraffin: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Immunohistochemical analysis of Sulfatase 2 in non-small-cell lung carcinoma. (left) lung squamous cell carcinoma stained with 2B4 antibody ...read more
Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Analysis using the FITC conjugate of NBP1-36727. Staining of Human lung cancer line with Sulfatase2-FITC. Image from verified customer review.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, IP, CyTOF-ready
Clone
2B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Sulfatase-2/SULF2 Antibody (2B4) Summary

Immunogen
Partial recombinant human Sulfatase 2 isoform b [Accession# NP_940998].
Localization
Endoplasmic reticulum
Specificity
Does not recognize human or mouse Sulfatase 1 or other family members.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SULF2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunohistochemistry 1:250-1:500
  • Immunohistochemistry-Paraffin 1:250-1:500
  • Immunoprecipitation 1:10-1:100
  • CyTOF-ready
Application Notes
This Sulfatase 2 (2B4) antibody is useful for Western Blot, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and ELISA. For WB a band can be seen at 50 kDa. Other bands may be seen at 135 kDa (unprocessed protein) and 37 kDa (fragment of 50 kDa subunit). For ELISA, use this antibody at 1-10 ug/ml for capture and 1-5 ug/ml for detection. It has been reported that NBP1-36727 picks up a 130 kDa band that is not Sulfatase 2 in human plasma. Because of this we do not recommend NBP1-36727 for capture of the human protein. Flow data from customer review on FITC-conjugated antibody.
Publications
Read Publications using
NBP1-36727 in the following applications:

  • IHC
    2 publications
  • 1 publication
  • WB
    3 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Sulfatase-2/SULF2 Antibody (2B4)

  • DKFZp313E091
  • EC 3.1.6
  • EC 3.1.6.-
  • EC 3.1.6.14
  • extracellular sulfatase Sulf-2
  • FLJ44488
  • FLJ90554
  • hSulf-2
  • KIAA1247HSULF-2
  • MGC126411
  • SULF2
  • SULF-2
  • sulfatase 2
  • Sulfatase2
  • Sulfatase-2

Background

Sulfatase 2 (SULF2) is a member of sulfatase family and catalyzes the removal of 6-O-sulfate groups from the heparan sulfate (HS) disaccharide units of heparan sulfate proteoglycans (HSPGs). HSPGs are present on the cell surface as well as in the extracellular matrix and act either as sequestration sites for growth factor/cytokine ligands or as coreceptors in multiple signal transduction pathways. SULF2 mediated desulfation of HSPGs is proposed to decrease the affinity of HSPGs for a variety of signaling ligands, releasing them from sequestration sites and making them available for triggering signaling stimuli. SULF2 is localized in endoplasmic reticulum, Golgi apparatus and also on the cell surface, and is expressed at highest levels in tissues such as ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta. In its post-translational modification, the conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. SULF2 enzyme regulates the activities of a number of signal transduction pathways which are up-regulated in many tissues during development and disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Sulfatase-2/SULF2 Antibody (NBP1-36727)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(2)
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species

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Product General Protocols

View specific protocols for Sulfatase-2/SULF2 Antibody (NBP1-36727): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Sulfatase-2/SULF2 Antibody (NBP1-36727). (Showing 1 - 1 of 1 FAQs).

  1. Would you please let me know if the immunogen region of NBP1-36727 is before position 539aa or beyond this position?
    • NBP1-36727 utilizes the full-length protein as the immunogen and unfortunately, has not been epitope mapped.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-36727AF405
Alexa Fluor 488 Labeled NBP1-36727AF488
Alexa Fluor 647 Labeled NBP1-36727AF647
Alexa Fluor 700 Labeled NBP1-36727AF700
Biotin Labeled NBP1-36727B
DyLight 405 Labeled NBP1-36727V
DyLight 488 Labeled NBP1-36727G
DyLight 550 Labeled NBP1-36727R
DyLight 650 Labeled NBP1-36727C
FITC Labeled NBP1-36727F
HRP Labeled NBP1-36727H

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Bioinformatics

Gene Symbol SULF2
Entrez
Uniprot