Sulfatase-2/SULF2 Antibody (2B4)

Western Blot: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Analysis showing Sulfatase 2 expression in MCF7 conditioned media (CM). In CM, the 75 kDa band is dominant (Tang R, Rosen SD. J Biol Chem. 2009 Aug 7;284(32):21505-14). Image courtesy of Mark Singer at UCSF.

Immunohistochemistry-Paraffin: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Immunohistochemical analysis of Sulfatase 2 in non-small-cell lung carcinoma. (left) lung squamous cell carcinoma stained with 2B4 antibody and (right) tumor-associated stromal cells stained with 2B4 antibody. Image courtesy of Mark Singer at UCSF.

Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Analysis using the FITC conjugate of NBP1-36727. Staining of Human lung cancer line with Sulfatase2-FITC. Image from verified customer review.

Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - An intracellular stain was performed on HeLa cells with Sulfatase-2/SULF2 Antibody (2B4) NBP1-36727AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, IHC, IHC-P, IP, CyTOF-ready
• Clone: 2B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Sulfatase-2/SULF2 Antibody (2B4) Summary

• Immunogen: Partial recombinant human Sulfatase 2 isoform b [Accession# NP_940998].
• Localization: Endoplasmic reticulum
• Specificity: Does not recognize human or mouse Sulfatase 1 or other family members.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SULF2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunohistochemistry 1:250-1:500
  • Immunohistochemistry-Paraffin 1:250-1:500
  • Immunoprecipitation 1:10-1:100
  • CyTOF-ready
• Application Notes: This Sulfatase 2 (2B4) antibody is useful for Western Blot, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and ELISA. For WB a band can be seen at 50 kDa. Other bands may be seen at 135 kDa (unprocessed protein) and 37 kDa (fragment of 50 kDa subunit). For ELISA, use this antibody at 1-10 ug/ml for capture and 1-5 ug/ml for detection. It has been reported that NBP1-36727 picks up a 130 kDa band that is not Sulfatase 2 in human plasma. Because of this we do not recommend NBP1-36727 for capture of the human protein. Flow data from customer review on FITC-conjugated antibody.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Sulfatase-2/SULF2 Antibody (2B4)

• DKFZp313E091
• EC 3.1.6
• EC 3.1.6.-
• EC 3.1.6.14
• extracellular sulfatase Sulf-2
• FLJ44488
• FLJ90554
• hSulf-2
• KIAA1247HSULF-2
• MGC126411
• SULF2
• SULF-2
• sulfatase 2
• Sulfatase2
• Sulfatase-2

Background

Sulfatase 2 (SULF2) is a member of sulfatase family and catalyzes the removal of 6-O-sulfate groups from the heparan sulfate (HS) disaccharide units of heparan sulfate proteoglycans (HSPGs). HSPGs are present on the cell surface as well as in the extracellular matrix and act either as sequestration sites for growth factor/cytokine ligands or as coreceptors in multiple signal transduction pathways. SULF2 mediated desulfation of HSPGs is proposed to decrease the affinity of HSPGs for a variety of signaling ligands, releasing them from sequestration sites and making them available for triggering signaling stimuli. SULF2 is localized in endoplasmic reticulum, Golgi apparatus and also on the cell surface, and is expressed at highest levels in tissues such as ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta. In its post-translational modification, the conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. SULF2 enzyme regulates the activities of a number of signal transduction pathways which are up-regulated in many tissues during development and disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sulfatase-2/SULF2 Antibody (NBP1-36727)(6)

Publications using NBP1-36727

• Yue X. Epithelial Deletion of Sulf2 Exacerbates Bleomycin-induced Lung Injury, Inflammation and Mortality Am. J. Respir. Cell Mol. Biol. 2017 Jun 28 [PMID: 28657777] (IHC, Mouse)
• Zhu C, He L, Zhou X et al. Sulfatase 2 promotes breast cancer progression through regulating some tumor-related factors. Oncology reports. 2015 Dec 02 [PMID: 26708018] (WB, Human)
  
  Details:
  Sulf2 antibody used for WB on various breast cancer cell lines (MCF-7, MDA-MB-231, MDA-MB-468 and BT-549) and HBL-100 (mammary epithelial cell line) (Figure 1).
• Solari V, Borriello L, Turcatel G et al. MYCN-dependent Expression of Sulfatase-2 Regulates Neuroblastoma Cell Survival Cancer Res. 2014 Aug 27 [PMID: 25164011]
• Yue X, Lu J, Auduong L et al. Overexpression of Sulf2 in idiopathic pulmonary fibrosis. Glycobiology 2013 Feb 14 [PMID: 23418199] (WB, Human)
• Lui NS, van Zante A, Rosen SD et al. SULF2 expression by immunohistochemistry and overall survival in oesophageal cancer: a cohort study BMJ Open 2012 Nov 23 [PMID: 23180455] (IHC, Human)
• Lemjabbar-Alaoui H, van Zante A, Singer MS, Xue Q, Wang YQ, Tsay D, He B, Jablons DM, Rosen SD. Sulf-2, a heparan sulfate endosulfatase, promotes human lung carcinogenesis. Oncogene;29(5):635-46. 2010 Feb 4. [PMID: 19855436] (WB, IHC-P, Human)

FAQs for Sulfatase-2/SULF2 Antibody (NBP1-36727). (Showing 1 - 1 of 1 FAQs).

1. Would you please let me know if the immunogen region of NBP1-36727 is before position 539aa or beyond this position?
   • NBP1-36727 utilizes the full-length protein as the immunogen and unfortunately, has not been epitope mapped.

Bioinformatics

• Gene Symbol: SULF2
• Entrez:
  • 55959(Human)
  • 72043(Mouse)
  • 311642(Rat)
• Uniprot:
  • NP_940998()