Western Blot: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Analysis showing Sulfatase 2 expression in MCF7 conditioned media (CM). In CM, the 75 kDa band is dominant (Tang R, Rosen SD. J Biol Chem. 2009 Aug ...read more
Immunohistochemistry-Paraffin: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Immunohistochemical analysis of Sulfatase 2 in non-small-cell lung carcinoma. (left) lung squamous cell carcinoma stained with 2B4 antibody ...read more
Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Analysis using the FITC conjugate of NBP1-36727. Staining of Human lung cancer line with Sulfatase2-FITC. Image from verified customer review.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Western Blot 1:1000
This Sulfatase 2 (2B4) antibody is useful for Western Blot, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and ELISA. For WB a band can be seen at 50 kDa. Other bands may be seen at 135 kDa (unprocessed protein) and 37 kDa (fragment of 50 kDa subunit). For ELISA, use this antibody at 1-10 ug/ml for capture and 1-5 ug/ml for detection. It has been reported that NBP1-36727 picks up a 130 kDa band that is not Sulfatase 2 in human plasma. Because of this we do not recommend NBP1-36727 for capture of the human protein. Flow data from customer review on FITC-conjugated antibody.
Read Publications using NBP1-36727 in the following applications:
Alternate Names for Sulfatase-2/SULF2 Antibody (2B4)
extracellular sulfatase Sulf-2
Sulfatase 2 (SULF2) is a member of sulfatase family and catalyzes the removal of 6-O-sulfate groups from the heparan sulfate (HS) disaccharide units of heparan sulfate proteoglycans (HSPGs). HSPGs are present on the cell surface as well as in the extracellular matrix and act either as sequestration sites for growth factor/cytokine ligands or as coreceptors in multiple signal transduction pathways. SULF2 mediated desulfation of HSPGs is proposed to decrease the affinity of HSPGs for a variety of signaling ligands, releasing them from sequestration sites and making them available for triggering signaling stimuli. SULF2 is localized in endoplasmic reticulum, Golgi apparatus and also on the cell surface, and is expressed at highest levels in tissues such as ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta. In its post-translational modification, the conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. SULF2 enzyme regulates the activities of a number of signal transduction pathways which are up-regulated in many tissues during development and disease.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.