Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody

Western blot shows lysates of H4 human neuroglioma cell line. PVDF Membrane was probed with 1 µg/mL of Human HS6ST1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5057) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HS6ST1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody Summary

• Immunogen: CHO cell-derived recombinant human HS6ST1
  Pro28-Trp401
  Accession # AAY14736
• Specificity: Detects human HS6ST1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse HS6ST3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: HS6ST1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody

• DKFZp547H098
• EC 2.8.2
• EC 2.8.2.-
• FLJ25392
• heparan sulfate 6-O-sulfotransferase 1
• heparan-sulfate 6-sulfotransferase
• HS6ST1
• HS6ST-1
• HS6STheparan-sulfate 6-O-sulfotransferase 1
• MGC116899
• MGC116901

Background

Heparan Sulfate is a highly sulfated polysaccharide that can be found on cell surface and extracellular matrix. It is usually covalently attached to a protein core as the glycan component of a proteoglycan. Heparan Sulfate interacts with a variety of proteins, such as growth factors, protease inhibitors, cytokines, lipoprotein lipase and viral envelope proteins, thus plays roles from cell growth, cell differentiation, cell motility, blood coagulation, lipid metabolism to viral infection (1, 2). Heparan Sulfate consists of repeating residues of uronic acid and N‑acetylglucosamine. The uronic acid residues can be sulfated at 2-O position by Heparan Sulfate 2-O Sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at N-, 3-O, and 6-O positions by N‑deacetylase/N‑sulfotransferases (NDSTs), Heparan Sulfate 3-O and 6-O sulfotransferases (HS3STs and HS6STs) respectively. However, the reactions catalyzed by these sulfotransferases are normally incomplete on the whole chain of Heparan Sulfate. As a result, Heparan Sulfate displays enormous sequence diversity that allows it to interact with a wide spectrum of proteins differently. Among three HS6STs, HS6ST1 is the first to be cloned (3). Mice deficient of the HS6ST1 homologue gene showed embryonic lethality (4).

1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
2. Esko, J.D. and S.B. Selleck (2002) Annu. Rev. Biochem. 71:435.
3. Habuchi, H et al. (1998) J. Biol. Chem. 273:9208.
4. Habuchi, H et al. (2007) J. Biol. Chem. 282:15578.
5. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
6. MacRae, I.J. et al. (2000) Biochemistry 39:1613.
7. Wu, Z.L. et al. (2002) Faseb J. 16:539.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody (AF5057)(1)

Publication using AF5057

• BE Thacker, KJ Thorne, C Cartwright, J Park, K Glass, A Chea, BP Kellman, NE Lewis, Z Wang, A Di Nardo, ST Sharfstein, W Jeske, J Walenga, J Hogwood, E Gray, B Mulloy, JD Esko, CA Glass Multiplex genome editing of mammalian cells for producing recombinant heparin Metabolic engineering, 2022-01-14;0(0):. 2022-01-14 [PMID: 35038554] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: HS6ST1
• Uniprot:
  • AAY14736()