Sulfatase-2/SULF2 Antibody (2B4) [DyLight 405]

Images

 
There are currently no images for Sulfatase-2/SULF2 Antibody (NBP1-36727V).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
Clone
2B4
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Sulfatase-2/SULF2 Antibody (2B4) [DyLight 405] Summary

Immunogen
Partial recombinant human Sulfatase 2 isoform b [Accession# NP_940998].
Localization
Endoplasmic reticulum
Specificity
Does not recognize human or mouse Sulfatase 1 or other family members.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SULF2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Sulfatase 2 (2B4) antibody is useful for Western Blot, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and ELISA. For WB a band can be seen at 50 kDa. Other bands may be seen at 135 kDa (unprocessed protein) and 37 kDa (fragment of 50 kDa subunit). For ELISA, use this antibody at 1-10 ug/ml for capture and 1-5 ug/ml for detection. It has been reported that NBP1-36727 picks up a 130 kDa band that is not Sulfatase 2 in human plasma. Because of this we do not recommend NBP1-36727 for capture of the human protein.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Sulfatase-2/SULF2 Antibody (2B4) [DyLight 405]

  • DKFZp313E091
  • EC 3.1.6
  • EC 3.1.6.-
  • EC 3.1.6.14
  • extracellular sulfatase Sulf-2
  • FLJ44488
  • FLJ90554
  • hSulf-2
  • KIAA1247HSULF-2
  • MGC126411
  • SULF2
  • SULF-2
  • sulfatase 2
  • Sulfatase2
  • Sulfatase-2

Background

Sulfatase 2 (SULF2) is a member of sulfatase family and catalyzes the removal of 6-O-sulfate groups from the heparan sulfate (HS) disaccharide units of heparan sulfate proteoglycans (HSPGs). HSPGs are present on the cell surface as well as in the extracellular matrix and act either as sequestration sites for growth factor/cytokine ligands or as coreceptors in multiple signal transduction pathways. SULF2 mediated desulfation of HSPGs is proposed to decrease the affinity of HSPGs for a variety of signaling ligands, releasing them from sequestration sites and making them available for triggering signaling stimuli. SULF2 is localized in endoplasmic reticulum, Golgi apparatus and also on the cell surface, and is expressed at highest levels in tissues such as ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta. In its post-translational modification, the conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. SULF2 enzyme regulates the activities of a number of signal transduction pathways which are up-regulated in many tissues during development and disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00023213-D01P
Species: Hu
Applications: WB, ELISA
NBP2-23603
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF6585
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
AF2364
Species: Hu
Applications: WB, IHC
AF2119
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, ICC
H00347527-B01P
Species: Hu
Applications: WB, ELISA
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP
NBP2-13980
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
AF5057
Species: Hu
Applications: WB
232-FA/CF
Species: Hu
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P

Publications for Sulfatase-2/SULF2 Antibody (NBP1-36727V) (0)

There are no publications for Sulfatase-2/SULF2 Antibody (NBP1-36727V).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Sulfatase-2/SULF2 Antibody (NBP1-36727V) (0)

There are no reviews for Sulfatase-2/SULF2 Antibody (NBP1-36727V). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Sulfatase-2/SULF2 Antibody (NBP1-36727V) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-36727B
DyLight 488 Labeled NBP1-36727G
DyLight 550 Labeled NBP1-36727R
DyLight 650 Labeled NBP1-36727C
FITC Labeled NBP1-36727F
HRP Labeled NBP1-36727H

Additional Sulfatase-2/SULF2 Products

Bioinformatics Tool for Sulfatase-2/SULF2 Antibody (NBP1-36727V)

Discover related pathways, diseases and genes to Sulfatase-2/SULF2 Antibody (NBP1-36727V). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Sulfatase-2/SULF2 Antibody (NBP1-36727V)

Discover more about diseases related to Sulfatase-2/SULF2 Antibody (NBP1-36727V).
 

Pathways for Sulfatase-2/SULF2 Antibody (NBP1-36727V)

View related products by pathway.

PTMs for Sulfatase-2/SULF2 Antibody (NBP1-36727V)

Learn more about PTMs related to Sulfatase-2/SULF2 Antibody (NBP1-36727V).
 

Research Areas for Sulfatase-2/SULF2 Antibody (NBP1-36727V)

Find related products by research area.

Blogs on Sulfatase-2/SULF2

There are no specific blogs for Sulfatase-2/SULF2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Sulfatase-2/SULF2 Antibody (2B4) [DyLight 405] and receive a gift card or discount.

Bioinformatics

Gene Symbol SULF2
Entrez