STAT3 Antibody

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, Raji human Burkitt's lymphoma cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human STAT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF17991) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for STAT3 at approximately 92 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

STAT3 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 15 µg/mL Sheep Anti-Human STAT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF17991) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Regulation of hepcidin by IL-10 in primary macrophages, showing (a) the effect of IL-10 on Hepcidin mRNA synthesis with and without anti-IL-10 blocking antibody; (b) Western blot of STAT3 phosphorylation 20 minutes after exposure to increasing concentrations of IL-10; (c) STAT3-P inhibition of IL-10 (30 ng/mL) by Stattic.Bar plots show data from at least 3 independent experiments and the sho Western blot shows data from a representative experiment. M = media, iso ctrl = isotype control. * P<0.05, ***P<0.01 (Mann-Whitney test, compared with Media control). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24520384), licensed under a CC-BY license. Not internally tested by R&D Systems.

Regulation of hepcidin by IL-10 in primary macrophages, showing (a) the effect of IL-10 on Hepcidin mRNA synthesis with and without anti-IL-10 blocking antibody; (b) Western blot of STAT3 phosphorylation 20 minutes after exposure to increasing concentrations of IL-10; (c) STAT3-P inhibition of IL-10 (30 ng/mL) by Stattic.Bar plots show data from at least 3 independent experiments and the sho Western blot shows data from a representative experiment. M = media, iso ctrl = isotype control. * P<0.05, ***P<0.01 (Mann-Whitney test, compared with Media control). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24520384), licensed under a CC-BY license. Not internally tested by R&D Systems.

Product Discontinued

STAT3 Antibody Summary

• Immunogen: E. coli-derived recombinant human STAT3
  Met1-Asn175
  Accession # P40763
• Specificity: Detects human and mouse STAT3 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: STAT3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT3 Antibody

• Acute-phase response factor
• APRFMGC16063
• DNA-binding protein APRF
• FLJ20882
• HIES
• signal transducer and activator of transcription 3 (acute-phase responsefactor)
• signal transducer and activator of transcription 3
• STAT3

Background

Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. Janus kinases (JAKs) phosphorylate STAT proteins and induce dimerization. Homo- or heterodimers translocate to the nucleus where they bind to DNA and activate transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAT3 Antibody (AF17991)(2)

Publications using AF17991

• Huang H, Lamikanra A, Alkaitis M, Thezenas M, Ramaprasad A, Moussa E, Roberts D, Casals-Pascual C Interleukin-10 regulates hepcidin in Plasmodium falciparum malaria. PLoS ONE, 2014-02-10;9(2):e88408. 2014-02-10 [PMID: 24520384] (Western Blot, Human)
• Bauer K, Kretzschmar AK, Cvijic H, Blumert C, Loffler D, Brocke-Heidrich K, Schiene-Fischer C, Fischer G, Sinz A, Clevenger CV, Horn F Cyclophilins contribute to Stat3 signaling and survival of multiple myeloma cells. Oncogene, 2009-06-08;28(31):2784-95. 2009-06-08 [PMID: 19503092] (ChIP, Human)

Bioinformatics

• Gene Symbol: STAT3
• Uniprot:
  • P40763()