STAT3 Antibodies: Novus Biologicals

MAB1799

STAT3 Antibody (232209) [Unconjugated]

100 μg

MAB1799

HAF018). A specific band was detected for STAT3 at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NCI‑H1703 human non-small cell lung carcinoma cell line, C6 rat glioma cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for STAT3 at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NCI‑H1703 human non-small cell lung carcinoma cell line, C6 rat glioma cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for STAT3 at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

STAT3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_lightbox">

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody (Catalog # <a class=

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_thumb" />

MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox">

HeLa human cell line was stained with Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody (Catalog # <a class=

MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Genetic Strategies Validation. </b>STAT3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with IFN-alpha 1, but is not detected in STAT3 knockout (KO) HeLa cell line using Mouse Anti-Human/ Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF018). A specific band was detected for STAT3 at approximately 80 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the STAT3 knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line, STAT1 knockout (KO) HeLa cell line, STAT2 KO HeLa cell line, STAT3 KO HeLa cell line, STAT5a KO HeLa cell line, STAT5b KO HeLa cell line, STAT6 KO HeLa cell line,. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for STAT3 at approximately 80 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the STAT3 knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the STAT3 knockout HeLa cell line was observed. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox">

<b>Genetic Strategies Validation. </b>STAT3 knockout HeLa human cervical epithelial cell line was stained with Mouse Anti-Human/Mouse STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody (Catalog # <a class=

MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the STAT3 knockout HeLa cell line was observed. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

Effects of kurarinone on the phosphorylation of p-STAT1 and p-STAT3 in lymph nodes. Single cell suspensions were collected from ILNs on day 42, the protein expression levels of p-STAT1, STAT1, p-STAT3, and STAT3 were measured using Western blots. (A) Representative images of Western blot and (B) Densitometric analysis for protein expressions was performed using ImageJ software. Data are presented as mean ± SEM of 6 mice from one of three experiments. (*) p < 0.05, (**) p < 0.01, (***) p < 0.001 versus vehicle-treated CIA mice group (One Way ANOVA followed by Tukey’s multiple comparison test). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33924467), licensed under a CC-BY license. Not internally tested by R&D Systems.

Galectin-3 expression induces activation of PYK2, STAT1 and GSK3 alpha / beta signalling. Expression of 37 protein kinases in SW620 cells in response to 10 µg/ml galectin-3 or BSA for 0.5 h was assessed by Proteome Profiler Human Phospho-Kinase Array (A, Percentage changes of the kinases in cell response to galectin-3 in comparison to control are shown at the bottom panel). The presence of galectin-3 increases the phosphorylation of PYK2, GSK3 alpha / beta , and STAT1 and decreases phosphorylation of STAT3. SW620 cells treated with 10 µg/ml galectin-3 for different times were assessed by immunoblotting using antibodies against p-PYK2, p-STAT-1, p-GSK3 alpha / beta or p-STAT-3 (B). The blots were striped and reprobed with antibodies against PYK2, STAT-1, GSK3 alpha / beta or STAT-3. The band density was quantified and expressed as percentages of phospho-/non-phosphorylated proteins (C). In D and E, SW620 cells were treated with 10 µg/ml galectin-3 or BSA followed by introduction of GSK3 alpha / beta inhibitor SB 216763 (SB) or PKY2 inhibitor PF-431396 (PF) for 15 min and the levels of phosphorylated PYK2, STAT-1, GSK3 alpha / beta or STAT-3 were analysed by immunoblotting. The blots were striped and reprobed with antibodies against PYK2, STAT-1, GSK3 alpha / beta or STAT-3. The densities of the blots from three independent experiments were quantified and are expressed as the percentage of phosphorylated/non-phosphorylated levels of each protein. ***P < 0.001, **P < 0.01, *P < 0.05 (ANOVA). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37055381), licensed under a CC-BY license. Not internally tested by R&D Systems.

Aspirin modulated the JAK/p-STAT3 signaling pathway in atypical hyperplastic intestinal mucosal cells of UC mice (n = 4 for each group). (A) Western blotting images for JAK/p-STAT3 signaling pathway-associated molecules expression. (B) Statistical analysis and comparison for p-STAT3 expression. (C) Statistical analysis and comparison for STAT3 expression. (D) Statistical analysis and comparison for cyclin D1 expression. (E) Statistical analysis and comparison for SOCS3 expression. *P <.05 versus control group. #P <.05 versus UC model group. JAK, Janus kinase; UC, ulcerative colitis; p-STAT3, phosphorylated-STAT3; STAT3, signal transducer and activator of transcription 3; SOCS3, suppressor of cytokine signaling 3. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35946886), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC1799U-100UG
Alexa Fluor 405	IC1799V-100UG
Alexa Fluor 488	IC1799G-100UG
Alexa Fluor 532	FAB1799X
Alexa Fluor 594	IC1799T-100UG
Alexa Fluor 647	IC1799R-100UG
Alexa Fluor 700	IC1799N-100UG
Alexa Fluor 750	IC1799S-100UG
Alexa Fluor Plus 405	FAB1799AFP405-100UG
Alexa Fluor Plus 488	FAB1799AFP488-100UG
Alexa Fluor Plus 555	FAB1799AFP555-100UG
Alexa Fluor Plus 594	FAB1799AFP594-100UG
Alexa Fluor Plus 647	FAB1799AFP647-100UG
Alexa Fluor Plus 680	FAB1799AFP680-100UG
Allophycocyanin	FAB1799A
Biotin	FAB1799B
CoraFluor 1	FAB1799CL1
DyLight 350	FAB1799D
DyLight 405	FAB1799E
DyLight 488	FAB1799K
DyLight 550	FAB1799L
DyLight 594	FAB1799M
DyLight 650	FAB1799W
DyLight 680	FAB1799Y
DyLight 755	FAB1799Z
Fluorescein	IC1799F
HRP	FAB1799H
Janelia Fluor 525	FAB1799JF525
Janelia Fluor 549	FAB1799I
Janelia Fluor 646	FAB1799J
Janelia Fluor 669	FAB1799JF669
PerCP	FAB1799C
Phycoerythrin	IC1799P
mFluor Violet 450 SE	FAB1799MFV450
mFluor Violet 500 SE	FAB1799MFV500
mFluor Violet 610 SE	FAB1799MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC1799P
Carrier Free	IC1799F
Carrier Free	MAB1799-SP
Carrier Free	IC1799V-100UG
Carrier Free	IC1799U-100UG
Carrier Free	IC1799T-100UG
Carrier Free	IC1799S-100UG
Carrier Free	IC1799R-100UG
Carrier Free	IC1799N-100UG
Carrier Free	IC1799G-100UG

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NBP2-22471

STAT3 Antibody (9D8)

(1 Review)

(5 Publications)

100 uL

NBP2-22471

Immunohistochemistry-Paraffin: STAT3 Antibody (9D8) [NBP2-22471] - Biopsies of normal and cancer tissues.

<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: STAT3 Antibody (9D8) [NBP2-22471] - Analysis performed using cross-linked chromatin from rat hepatoma cells treated with insulin. IP performed using a multiplex microplate Matrix ChIP assay with STAT3 monoclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data done in quadruplicate using 1ul of DNA in 2ul SYBR real-time PCR reactions containing primers to amplify exon-1 or exon-28 of LAMC1.Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the rat LAMC1 locus is shown; boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by LAMC1 primers are represented by black bars. Data courtesy of the Innovators Program.

Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of 25ug HepG2 total lysate.

Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of 25ug of various whole cell lysates.

Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of 25ug of U2-OS lysate from STAT3 SMART pool siRNA transfected or non-targeting control transfected U2-OS cells onto a 4-20% Tris-HCl polyacrylamide gel.

Immunoprecipitation: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of STAT3 was performed on HepG2 cells. The antigen: antibody complex was formed by incubating 750ug whole cell lysate with 2ug of mouse monoclonal antibody recognizing STAT3 overnight on a rocking platform at 4C. The immune-complex was then captured on 50ul Protein A/G Plus Agarose. Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing STAT3 at a dilution of 1:5000 overnight rotating at 4C. Membranes were washed in TBST and probed with Clean-blot IP detection reagent at a dilution of 1:2000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura.

N-EV & H-EV treatment promote macrophage M2 polarization by delivering miR-21-5p that targets PTEN. a, western blot analysis of PTEN protein expression level in induced macrophages. H/i-miR-EV, monocytes were induced with the presence of EV secreted by miR-21-5p-inhibited, hypoxia pre-challenged MSCs; H-EV + i-miR, monocytes were transfected with miR-21-5p inhibitor-expressing vector before induction with the presence of H-EV. Macrophages induced without MSC-EV were used as negative control (NC). b, c, flow cytometry determining the percentage of CD163+CD206+ cells among total CD68+ cells after induction. N-EV + O/E PTEN or H-EV + O/E PTEN, monocytes were transfected with PTEN overexpressing vector before N-EV or H-EV treatment, respectively. d–f, western blot detecting Akt & STAT3 protein expression as well as their activating phosphorylation (p-Ser473 for Akt & p-tyr705 for STAT3) in macrophages after induction. g–i, ELISA evaluating IL-10, TGF-beta & VEGF-alpha in macrophage culture medium after induction. Macrophages induced with the presence of N-EV were used as negative control in b–i. Tukey’s test was used for statistical analysis. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30736829), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm, Pm

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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AF4607

STAT3 [p Tyr705] Antibody [Unconjugated]

(1 Review)

(11 Publications)

100 μg

AF4607

11100-1) for 20 minutes or 50 µg/mL Recombinant Human IL-22 (Catalog # 782-IL) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-STAT3 (Y705) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4607) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT3 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # <a class=

11100-1) for 20 minutes or 50 µg/mL Recombinant Human IL-22 (Catalog # 782-IL) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-STAT3 (Y705) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4607) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT3 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes or 50 µg/mL Recombinant Human IL-22 (Catalog # 782-IL) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-STAT3 (Y705) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4607) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT3 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_lightbox">

<b>Biological Strategies Validation. </b>Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 U/mL rhIFN-alpha for 20 minutes (dark orange filled histogram) was stained with Human Phospho-STAT3 (Y705) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4607) or control antibody (Catalog # <a class=

AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_thumb" />

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>Phospho-STAT3 was detected in immersion fixed IFN-alpha treated Daudi human Burkitt's lymphoma cell line using 10 µg/mL Human Phospho-STAT3 (Y705) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4607) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # <a class=

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 µg/mL Recombinant Human IL‑22 (Catalog # <a class=NoLineLink href='//www.rndsystems.com/product_results.aspx?k=782-IL'>782-IL</a>) for 15 minutes, loaded at 0.2 mg/ml. A specific band was detected for STAT3 at approximately 91 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-STAT3 (Y705) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4607). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

<b>Biological Strategies Validation. </b>IL-22BPi1 does not interact with IL-22 or IL-22BPi2. (A) A549 cells were exposed for 30 minutes to dilutions of IL-22-containing culture medium (CM) previously produced by IL22-transfected HEK293 cells. A549 cell lysates (CL) were immunoblotted for pSTAT3 and tubulin as loading control. The relative densitometry of pSTAT3 normalized to that of tubulin is also represented. (B) A549 cells were treated with the optimum IL-22 dilution from A (1/512) for different periods of time, lysed and immunoblotted for pSTAT3. An unspecific protein band (u.p.) was used as loading control. (C) IL-22BP concentration in conditioned medium (CM) of transfected HEK293 cells was measured by ELISA, and the indicated amounts in nanograms (ng) of IL-22BPi1 or IL-22BPi2 were pre-incubated for 1 h at 37°C with the selected IL-22 concentration from (A). A549 were exposed to the pre-incubated combinations for 20 min. An excess of IL-22BPi2 was used as phosphorylation blocking control (lane 3). Cells were lysed and immunoblotted for pSTAT3 and actin as loading control. (D) HeLa cells were co-transfected with the indicated expression plasmids, 24 h later cells were lysed and the CM was subjected to acetone precipitation (AP), and proteins were immunoblotted for FLAG. Intracellular IL-22BPi1 is indicated with dark purple arrows, intracellular and secreted IL-22BPi2 is indicated with green arrows, and co-expressed IL-22 and IL-17 are also indicated. (E) Conditioned media (CM) from 3 independent experiments, in which expression vectors for the three IL-22BP isoforms were individually transfected into HEK293 cells together with either IL-17 or IL-22 expression vectors or an empty vector control (EV), were analyzed 24 h after transfection by ELISA for IL-22BP (mean ± SEM; n = 3; **p < 0.01 by unpaired t-test). (F) IL-22BPi1 does not interact with IL-22BPi2. IL-22BPi1-MF expression plasmid containing Myc and FLAG tags was co-transfected with an inducible pTRE3G-based vector expressing IL-22BPi2 with onl

N-EV and H-EV treatment promote macrophage M2 polarization by delivering miR-21-5p that targets PTEN. a, western blot analysis of PTEN protein expression level in induced macrophages. H/i-miR-EV, monocytes were induced with the presence of EV secreted by miR-21-5p-inhibited, hypoxia pre-challenged MSCs; H-EV + i-miR, monocytes were transfected with miR-21-5p inhibitor-expressing vector before induction with the presence of H-EV. Macrophages induced without MSC-EV were used as negative control (NC). b, c, flow cytometry determining the percentage of CD163+CD206+ cells among total CD68+ cells after induction. N-EV + O/E PTEN or H-EV + O/E PTEN, monocytes were transfected with PTEN overexpressing vector before N-EV or H-EV treatment, respectively. d–f, western blot detecting Akt and STAT3 protein expression as well as their activating phosphorylation (p-Ser473 for Akt and p-tyr705 for STAT3) in macrophages after induction. g–i, ELISA evaluating IL-10, TGF-beta and VEGF-alpha in macrophage culture medium after induction. Macrophages induced with the presence of N-EV were used as negative control in b–i. Tukey’s test was used for statistical analysis. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30736829), licensed under a CC-BY license. Not internally tested by R&D Systems.

Interleukin 6 (IL-6) and STAT3 expression in BC cells. (A) IL-6 levels were measured by ELISA assay in supernatants of MCF-10, MCF-7, MDA-MB-231, BT-549, MDA-MB-157 and MDA-MB-453 cells. IL-6 mRNA relative levels of MCF-10, MCF-7, MDA-MB-231, BT-549, MDA-MB-157 and MDA-MB-453 cells were also recorded. (B) Expression of activated STAT3 (STAT3pY705) in MCF-10, MCF-7, MDA-MB-231, BT-549, MDA-MB-157 and MDA-MB-453 cells was analyzed by flow cytometry (gray peak). As the negative control, the secondary antibody alone was used (white peak). (C) STAT3 mRNA relative levels of MCF-10, MCF-7, MDA-MB-231, BT-549, MDA-MB-157 and MDA-MB-453 cells. (D) STAT3 phosphorylation levels (STAT3pY705-dark gray peak with tracing line) were analyzed in MDA-MB-231 and MDA-MB-157 cells treated with Tocilizumab (TCZ), recombinant IL-6 (rIL-6) and STAT3 inhibitor, STATTIC (STC). As the negative control, the secondary antibody alone was used (white peak). * p < 0.05; ** p < 0.001. Three independent experiments were performed. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35626741), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF4607U-100UG
Alexa Fluor 405	AF4607V-100UG
Alexa Fluor 488	AF4607G-100UG
Alexa Fluor 532	AF4607X-100UG
Alexa Fluor 594	AF4607T-100UG
Alexa Fluor 647	AF4607R-100UG
Alexa Fluor 700	AF4607N-100UG
Alexa Fluor 750	AF4607S-100UG
Alexa Fluor Plus 405	AF4607AFP405-100UG
Alexa Fluor Plus 488	AF4607AFP488-100UG
Alexa Fluor Plus 555	AF4607AFP555-100UG
Alexa Fluor Plus 594	AF4607AFP594-100UG
Alexa Fluor Plus 647	AF4607AFP647-100UG
Alexa Fluor Plus 680	AF4607AFP680-100UG
Allophycocyanin	AF4607A
Biotin	AF4607B
CoraFluor 1	AF4607CL1
DyLight 350	AF4607D
DyLight 405	AF4607E
DyLight 488	AF4607K
DyLight 550	AF4607L
DyLight 594	AF4607M
DyLight 650	AF4607W
DyLight 680	AF4607Y
DyLight 755	AF4607Z
FITC	AF4607F
HRP	AF4607H
Janelia Fluor 525	AF4607JF525
Janelia Fluor 549	AF4607I
Janelia Fluor 646	AF4607J
Janelia Fluor 669	AF4607JF669
PE	AF4607P
PerCP	AF4607C
mFluor Violet 450 SE	AF4607MFV450
mFluor Violet 500 SE	AF4607MFV500
mFluor Violet 610 SE	AF4607MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4607-SP

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NB100-799

STAT3 Antibody

(19 Publications)

0.1 mg

NB100-799

Flow Cytometry: STAT3 Antibody [NB100-799] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: STAT3 Antibody [NB100-799] - Staining of Mouse Thymus. Antibody at 4 ug/mL. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Immunohistochemistry-Paraffin: STAT3 Antibody [NB100-799] - Negative Control showing staining of Mouse Thymus, with no primary antibody.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NBP2-24463

STAT3 [p Tyr705] Antibody

(3 Reviews)

(5 Publications)

0.02 mg

NBP2-24463

<b>Biological Strategies Validation. </b>Western Blot: STAT3 [p Tyr705] Antibody [NBP2-24463] - Human breast cancer cells MDA-MB-231 were treated with carboplatin for 72 hours and the expression of p-Stat3 at Y705 and total Stat3 were detected. Image from verified customer review.

Reactivity:

Hu, Mu, Rt, Ca, Ch, Pm, Ma-Op

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Ch, Pm, Ma-Op

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6382A

Alt Catalog# IMG-6382A

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB4934

STAT3 [p Ser727] Antibody (788335) [Unconjugated]

(1 Review)

(5 Publications)

100 μg

MAB4934

206-IL) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-STAT3 (S727) Monoclonal Antibody (Catalog # MAB4934) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-STAT3 (S727) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-6 (Catalog # <a class=

206-IL) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-STAT3 (S727) Monoclonal Antibody (Catalog # MAB4934) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-STAT3 (S727) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-STAT3 (S727) Monoclonal Antibody (Catalog # MAB4934) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-STAT3 (S727) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules." class="big_lightbox">

<b>Biological Strategies Validation. </b>Daudi human Burkitt's lymphoma cell line was unstimulated (open histogram) or treated with 500 U/mL rhIFN-alpha for 20 minutes (filled histogram) and stained with Mouse anti-Human Phospho-STAT3 (S727) Molyclonal Antibody (Catalog # MAB4934) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules." class="big_thumb" />

Regulation of hepcidin by IL-10 in primary macrophages, showing (a) the effect of IL-10 on Hepcidin mRNA synthesis with and without anti-IL-10 blocking antibody; (b) Western blot of STAT3 phosphorylation 20 minutes after exposure to increasing concentrations of IL-10; (c) STAT3-P inhibition of IL-10 (30 ng/mL) by Stattic.Bar plots show data from at least 3 independent experiments and the sho Western blot shows data from a representative experiment. M = media, iso ctrl = isotype control. * P<0.05, ***P<0.01 (Mann-Whitney test, compared with Media control). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24520384), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC4934U-100UG
Alexa Fluor 405	IC4934V-100UG
Alexa Fluor 488	IC4934G-100UG
Alexa Fluor 532	FAB4934X
Alexa Fluor 594	IC4934T-100UG
Alexa Fluor 647	IC4934R-100UG
Alexa Fluor 700	IC4934N-100UG
Alexa Fluor 750	IC4934S-100UG
Alexa Fluor Plus 405	FAB4934AFP405-100UG
Alexa Fluor Plus 488	FAB4934AFP488-100UG
Alexa Fluor Plus 555	FAB4934AFP555-100UG
Alexa Fluor Plus 594	FAB4934AFP594-100UG
Alexa Fluor Plus 647	FAB4934AFP647-100UG
Alexa Fluor Plus 680	FAB4934AFP680-100UG
Allophycocyanin	FAB4934A
Biotin	FAB4934B
CoraFluor 1	FAB4934CL1
DyLight 350	FAB4934D
DyLight 405	FAB4934E
DyLight 488	FAB4934K
DyLight 550	FAB4934L
DyLight 594	FAB4934M
DyLight 650	FAB4934W
DyLight 680	FAB4934Y
DyLight 755	FAB4934Z
FITC	FAB4934F
HRP	FAB4934H
Janelia Fluor 525	FAB4934JF525
Janelia Fluor 549	FAB4934I
Janelia Fluor 646	FAB4934J
Janelia Fluor 669	FAB4934JF669
PE	FAB4934P
PerCP	FAB4934C
mFluor Violet 450 SE	FAB4934MFV450
mFluor Violet 500 SE	FAB4934MFV500
mFluor Violet 610 SE	FAB4934MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB4934-SP
Carrier Free	IC4934V-100UG
Carrier Free	IC4934U-100UG
Carrier Free	IC4934T-100UG
Carrier Free	IC4934S-100UG
Carrier Free	IC4934R-100UG
Carrier Free	IC4934N-100UG
Carrier Free	IC4934G-100UG

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NB100-82213

STAT3 [p Tyr705] Antibody

(1 Review)

0.05 ml

NB100-82213

Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NB100-82213] - Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue.

Immunohistochemistry-Paraffin: STAT3 [p Tyr705] Antibody [NB100-82213] - Immunohistochemical analysis of paraffin-embedded Rat-lung tissue.

Immunohistochemistry: STAT3 [p Tyr705] Antibody [NB100-82213] - Immunofluorescence analysis of mouse-spleen tissue.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-90159

Alt Catalog# IMG-90159

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-67433

STAT3 [p Ser727] Antibody (SY24-09)

(1 Review)

100 ul

NBP2-67433

Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of Phospho-STAT3(S727) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lane 1: NIH/3T3 cell lysate Lane 2: Jurkat cell lysate

<b>Biological Strategies Validation. </b>Western Blot: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Human U251 glioma cell line, whole cell lysates.Cells were treated with SP600125, DB07268, JNK-IN-8 and SB203580. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Staining Phospho-STAT3(S727) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Staining Phospho-STAT3(S727) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded human lung tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat kidney tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat liver tissue using anti-Phospho-STAT3(S727) antibody. Counter stained with hematoxylin.

<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat kidney tissue using anti-Phospho-STAT3 (S727) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: STAT3 [p Ser727] Antibody (SY24-09) [NBP2-67433] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-STAT3 [p Ser727] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1:500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-67432

STAT3 Antibody (SY24-08)

(1 Publication)

100 ul

NBP2-67432

<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: STAT3 Antibody (SY24-08) [NBP2-67432] - Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-STAT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-38, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: STAT3 Antibody (SY24-08) [NBP2-67432] - Analysis of Stat3 on different lysates using anti-Stat3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: SHG-44 Lane 3: SW480

Immunohistochemistry-Paraffin: STAT3 Antibody (SY24-08) [NBP2-67432] - Analysis of paraffin-embedded human colon cancer tissue using anti-Stat3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: STAT3 Antibody (SY24-08) [NBP2-67432] - Analysis of paraffin-embedded human pancreas tissue using anti-Stat3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: STAT3 Antibody (SY24-08) [NBP2-67432] - Analysis of paraffin-embedded mouse brain tissue using anti-Stat3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: STAT3 Antibody (SY24-08) [NBP2-67432] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Stat3 antibody. Counter stained with hematoxylin.

Reactivity:

Hu, Mu, Rt, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Ze

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB4607

STAT3 [p Tyr705] Antibody (805435)

(5 Publications)

100 μg

MAB4607

11100-1) for 20 minutes or 50 µg/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB4607) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

11100-1) for 20 minutes or 50 µg/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB4607) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes or 50 µg/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB4607) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

<b>Biological Strategies Validation. </b>TOFA decreases STAT3 but not STAT1 signaling. (A) Densitometric analysis of pSTAT1 and pSTAT3 protein expression in gastrocnemius. (B) Densitometric analysis of SOCS1 and SOCS3 protein expression in gastrocnemius. Data are normalized to endogenous control ( alpha -tubulin) and expressed as arbitrary units (A.U.). Representative cropped blots of two animals of each group are shown: healthy, e-RA, and e-RA+TOFA, respectively. Data are shown as the mean and SEM (n = 7 rabbits per group). *** p < 0.001 vs. healthy. e-RA: experimental rheumatoid arthritis; TOFA: tofacitinib. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37685986), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB4607U-100UG
Alexa Fluor 405	FAB4607V-100UG
Alexa Fluor 488	FAB4607G-100UG
Alexa Fluor 532	FAB4607X-100UG
Alexa Fluor 594	FAB4607T-100UG
Alexa Fluor 647	FAB4607R-100UG
Alexa Fluor 700	FAB4607N-100UG
Alexa Fluor 750	FAB4607S-100UG
Alexa Fluor Plus 405	FAB4607AFP405-100UG
Alexa Fluor Plus 488	FAB4607AFP488-100UG
Alexa Fluor Plus 555	FAB4607AFP555-100UG
Alexa Fluor Plus 594	FAB4607AFP594-100UG
Alexa Fluor Plus 647	FAB4607AFP647-100UG
Alexa Fluor Plus 680	FAB4607AFP680-100UG
Allophycocyanin	FAB4607A
Biotin	FAB4607B
CoraFluor 1	FAB4607CL1
DyLight 350	FAB4607D
DyLight 405	FAB4607E
DyLight 488	FAB4607K
DyLight 550	FAB4607L
DyLight 594	FAB4607M
DyLight 650	FAB4607W
DyLight 680	FAB4607Y
DyLight 755	FAB4607Z
FITC	FAB4607F
HRP	FAB4607H
Janelia Fluor 525	FAB4607JF525
Janelia Fluor 549	FAB4607I
Janelia Fluor 646	FAB4607J
Janelia Fluor 669	FAB4607JF669
PE	FAB4607P
PerCP	FAB4607C
mFluor Violet 450 SE	FAB4607MFV450
mFluor Violet 500 SE	FAB4607MFV500
mFluor Violet 610 SE	FAB4607MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB4607-SP

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NBP2-52936

STAT3 Antibody (CL0492) - BSA Free

0.1 ml

NBP2-52936

Western Blot: STAT3 Antibody (CL0492) [NBP2-52936] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4

Immunohistochemistry-Paraffin: STAT3 Antibody (CL0492) [NBP2-52936] - Staining of human endometrium shows strong nuclear and moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: STAT3 Antibody (CL0492) [NBP2-52936] - Staining of human kidney shows strong nuclear and moderate cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: STAT3 Antibody (CL0492) [NBP2-52936] - Staining of human rectum shows moderate to strong nuclear and cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: STAT3 Antibody (CL0492) [NBP2-52936] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Staining of A549 cells, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43941AF350
Alexa Fluor 405	NBP3-43941AF405
Alexa Fluor 488	NBP3-43941AF488
Alexa Fluor 532	NBP3-43941AF532
Alexa Fluor 594	NBP3-43941AF594
Alexa Fluor 647	NBP3-43941AF647
Alexa Fluor 700	NBP3-43941AF700
Alexa Fluor 750	NBP3-43941AF750
Alexa Fluor Plus 405	NBP3-43941AFP405
Alexa Fluor Plus 488	NBP3-43941AFP488
Alexa Fluor Plus 555	NBP3-43941AFP555
Alexa Fluor Plus 594	NBP3-43941AFP594
Alexa Fluor Plus 647	NBP3-43941AFP647
Alexa Fluor Plus 680	NBP3-43941AFP680
Allophycocyanin	NBP3-43941APC
Biotin	NBP3-43941B
CoraFluor 1	NBP3-43941CL1
DyLight 350	NBP3-43941UV
DyLight 405	NBP3-43941V
DyLight 488	NBP3-43941G
DyLight 550	NBP3-43941R
DyLight 594	NBP3-43941DL594
DyLight 650	NBP3-43941C
DyLight 680	NBP3-43941FR
DyLight 755	NBP3-43941IR
FITC	NBP3-43941F
HRP	NBP3-43941H
Janelia Fluor 525	NBP3-43941JF525
Janelia Fluor 549	NBP3-43941JF549
Janelia Fluor 646	NBP3-43941JF646
Janelia Fluor 669	NBP3-43941JF669
PE	NBP3-43941PE
PerCP	NBP3-43941PCP
mFluor Violet 450 SE	NBP3-43941MFV450
mFluor Violet 500 SE	NBP3-43941MFV500
mFluor Violet 610 SE	NBP3-43941MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-43941
BSA Free	NBP2-52936-25ul

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NBP1-48014

STAT3 Antibody (OTI21E7)

0.1 ml

NBP1-48014

Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] Staining of paraffin-embedded thyroid using anti-STAT3 mouse monoclonal antibody.

Western Blot: STAT3 Antibody (21E7) [NBP1-48014] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT3(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT3.

Western Blot: STAT3 Antibody (OTI21E7) [NBP1-48014] - Analysis of extracts (35ug) from different cell line by using anti-STAT3 monoclonal antibody. (1:500)

Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded breast using anti-STAT3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded Carcinoma of kidney using anti-STAT3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded Carcinoma of lung using anti-STAT3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded Carcinoma of pancreas using anti-STAT3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded colon using anti-STAT3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded kidney using anti-STAT3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded lung using anti-STAT3 mouse monoclonal antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-74378AF350
Alexa Fluor 405	NBP2-74378AF405
Alexa Fluor 488	NBP2-74378AF488
Alexa Fluor 532	NBP2-74378AF532
Alexa Fluor 594	NBP2-74378AF594
Alexa Fluor 647	NBP2-74378AF647
Alexa Fluor 700	NBP2-74378AF700
Alexa Fluor 750	NBP2-74378AF750
Alexa Fluor Plus 405	NBP2-74378AFP405
Alexa Fluor Plus 488	NBP2-74378AFP488
Alexa Fluor Plus 555	NBP2-74378AFP555
Alexa Fluor Plus 594	NBP2-74378AFP594
Alexa Fluor Plus 647	NBP2-74378AFP647
Alexa Fluor Plus 680	NBP2-74378AFP680
Allophycocyanin	NBP2-74378APC
Biotin	NBP2-74378B
CoraFluor 1	NBP2-74378CL1
DyLight 350	NBP2-74378UV
DyLight 405	NBP2-74378V
DyLight 488	NBP2-74378G
DyLight 550	NBP2-74378R
DyLight 594	NBP2-74378DL594
DyLight 650	NBP2-74378C
DyLight 680	NBP2-74378FR
DyLight 755	NBP2-74378IR
FITC	NBP2-74378F
HRP	NBP2-74378H
Janelia Fluor 525	NBP2-74378JF525
Janelia Fluor 549	NBP2-74378JF549
Janelia Fluor 646	NBP2-74378JF646
Janelia Fluor 669	NBP2-74378JF669
PE	NBP2-74378PE
PerCP	NBP2-74378PCP
mFluor Violet 450 SE	NBP2-74378MFV450
mFluor Violet 500 SE	NBP2-74378MFV500
mFluor Violet 610 SE	NBP2-74378MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-74378

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

STAT3 Antibodies

STAT3 Antibody (232209) [Unconjugated]

STAT3 Antibody (9D8)

STAT3 [p Tyr705] Antibody [Unconjugated]

STAT3 Antibody

STAT3 [p Tyr705] Antibody

STAT3 [p Ser727] Antibody (788335) [Unconjugated]

STAT3 [p Tyr705] Antibody

STAT3 [p Ser727] Antibody (SY24-09)

STAT3 Antibody (SY24-08)

STAT3 [p Tyr705] Antibody (805435)

STAT3 Antibody (CL0492) - BSA Free

STAT3 Antibody (OTI21E7)