Jak1 Antibody (413104) [Unconjugated]

Images

 
Jurkat human acute T cell leukemia cell line was stained with Rat Anti-Human/Mouse/Rat Jak1 Monoclonal Antibody (Catalog # MAB4260, filled histogram) or isotype control antibody (Catalog # MAB0061, open ...read more
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, A20 mouse B cell lymphoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was ...read more
Jak1 was detected in immersion fixed paraffin-embedded sections of human epidermis using 25 µg/mL Rat Anti-Human/Mouse/Rat Jak1 Monoclonal Antibody (Catalog # MAB4260) overnight at 4 °C. Tissue was stained ...read more
Jak1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rat Anti-Human/Mouse/Rat Jak1 Monoclonal Antibody (Catalog # MAB4260) at 10 µg/mL for 3 hours at room temperature. Cells ...read more
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Jak1 at approximately 136 kDa (as indicated) using 10 µg/mL of Rat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, CyTOF-ready, Flow, ICC/IF
Clone
413104
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Jak1 Antibody (413104) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Jak1
Pro32-Phe286
Accession # P23458
Specificity
Detects human, mouse, and rat Jak1.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
JAK1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB4260 in the following applications:

Publications
Read Publications using
MAB4260 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is sold under license from Millipore Corporation under the following US or foreign patents: 5,821,069; 5,658,791; EP0560890. This product shall not be used to commercially screen drug molecules being developed as JAK1 or JAK2 inhibitors. Any such activity will be outside the scope of the research use only label license.



This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jak1 Antibody (413104) [Unconjugated]

  • EC 2.7.10
  • Jak1
  • JAK-1
  • JAK1Atyrosine-protein kinase JAK1
  • JAK1B
  • Janus kinase 1EC 2.7.10.2
  • JTK3

Background

Janus Kinase 1 (Jak1) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak1 occurs via phosphorylation at two adjacent tyrosine residues, Y1022 and Y1023, within the kinase domain. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak1 is required for the activation of STAT1 and STAT2 in response to interferon alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Jak1 Antibody (MAB4260)(2)

Review for Jak1 Antibody (MAB4260) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB4260:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Jak1 MAB4260
Enlarge
5
reviewed by:
Verified Customer
IHC-P Human 04/04/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBreast cancer tissue
SpeciesHuman
LotZBB0215111

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Jak1 Antibody (MAB4260) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Jak1 Products

Blogs on Jak1.

Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

mFluor Violet Conjugated Antibodies

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Recent Reviews

5
5
1
4
0
3
0
2
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1
0

Verified Customer
04/04/2020
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol JAK1
Uniprot