SOCS-3 Antibody (516919) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, U937 human histiocytic lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse SOCS‑3 Monoclonal Antibody (Catalog # MAB5696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS‑3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 516919
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

SOCS-3 Antibody (516919) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SOCS-3
  Met1-Leu225
  Accession # O14543
• Specificity: Detects human or mouse SOCS-3 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOCS3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOCS-3 Antibody (516919) [Unconjugated]

• ATOD4
• CIS3
• CIS3CIS-3
• Cish3
• cytokine-induced SH2 protein 3
• Cytokine-inducible SH2 protein 3
• SOCS3
• SOCS-3
• SOCS-3STAT-induced STAT inhibitor 3
• SSI-3
• SSI-3ATOD4
• SSI3MGC71791
• suppressor of cytokine signaling 3

Background

Suppressor of cytokine signaling 3 (SOCS-3), also known as CIS3, is a widely expressed 30 kDa protein that inhibits signal transduction induced by multiple cytokines including Erythropoietin, IL-6, LIF, Oncostatin M, and Leptin. It binds downstream signaling molecules such as JAK kinases and targets them for ubiquitin-mediated degradation, essential in a negative feedback control loop. Human SOCS-3 shares 96% aa sequence identity with mouse and rat SOCS-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOCS-3 Antibody (MAB5696)(4)

Publications using MAB5696

• R Chakrabort, H Hu, C Darido, K Vickery, S Ranganatha ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation International Journal of Molecular Sciences, 2021-11-22;22(22):. 2021-11-22 [PMID: 34830441] (Western Blot, Human)
• R Chakrabort, K Vickery, C Darido, S Ranganatha, H Hu Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation International Journal of Molecular Sciences, 2021-08-12;22(16):. 2021-08-12 [PMID: 34445392] (Western Blot, Human)
• BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas J. Immunol, 2017-03-22;0(0):. 2017-03-22 [PMID: 28330899] (Western Blot, Mouse)
• Vartoukian S, Tilakaratne W, Seoudi N, Bombardieri M, Bergmeier L, Tappuni A, Fortune F Dysregulation of the suppressor of cytokine signalling 3-signal transducer and activator of transcription-3 pathway in the aetiopathogenesis of Sjogren's syndrome. Clin Exp Immunol, 2014-09-01;177(3):618-29. 2014-09-01 [PMID: 24827536] (IHC-P, Western Blot, Human)

Bioinformatics

• Gene Symbol: SOCS3
• Uniprot:
  • O14543()