Human Soluble gp130 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Soluble gp130 Quantikine ELISA Kit Summary

• Background: The Quantikine Human soluble gp130 Immunoassay is a 4.5 or 5.5 hour solid phase ELISA designed to measure sgp130 in cell culture supernates, serum, and plasma. It contains soluble insect cell-expressed recombinant human gp130 and antibodies raised against recombinant human gp130 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural gp130 showed li...near curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural gp130.
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• Specificity: Natural and recombinant human sgp130
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL6ST

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Soluble gp130 Quantikine ELISA Kit

• CD130 antigen
• CD130
• CDw130
• DKFZp564F053
• gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
• gp130
• IL-6 receptor subunit beta
• IL-6R subunit beta
• IL-6RB
• IL-6R-beta
• IL6ST
• interleukin 6 signal transducer (gp130, oncostatin M receptor)
• interleukin receptor beta chain
• interleukin-6 receptor subunit beta
• Interleukin-6 signal transducer
• Membrane glycoprotein 130
• membrane glycoprotein gp130
• Oncostatin-M receptor subunit alpha

Background

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the classI cytokine receptor family. Binding of IL-6 or IL-11 to either the membrane-anchored or soluble IL-6 R or IL-11 R, respectively, initiates the association of IL-6 R (IL-11 R) with gp130. The complex then undergoes homodimerization and signal transduction. For other IL-6 family cytokines, such as LIF and OSM, signal transduction is similarly triggered by the heterodimerization of gp130 with LIFR or OSM R.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for gp130/CD130 (DGP00)(15)

Publications using DGP00

• Stukas, S;Goshua, G;Conway, EM;Lee, AYY;Hoiland, RL;Sekhon, MS;Y C Chen, L; ABO blood group and COVID-19 severity: Associations with endothelial and adipocyte activation in critically ill patients PloS one 2025-04-02 [PMID: 40173171] (Human)
• SA Müller, MD Shmueli, X Feng, J Tüshaus, N Schumacher, R Clark, BE Smith, A Chi, S Rose-John, ME Kennedy, SF Lichtentha The Alzheimer's disease-linked protease BACE1 modulates neuronal IL-6 signaling through shedding of the receptor gp130 Molecular Neurodegeneration, 2023-02-21;18(1):13. 2023-02-21 [PMID: 36810097] (Human)
• V Bratseth, J Nordeng, R Helseth, S Solheim, S Åkra, H Arnesen, G Chiva-Blan, I Seljeflot Circulating Microvesicles in Association with the NLRP3 Inflammasome in Coronary Thrombi from STEMI Patients Biomedicines, 2022-09-05;10(9):. 2022-09-05 [PMID: 36140297] (Human)
• R Salemi, G Gattuso, B Tomasello, A Lavoro, A Gaudio, M Libra, SS Signorelli, S Candido Co-Occurrence of Interleukin-6 Receptor Asp358Ala Variant and High Plasma Levels of IL-6: An Evidence of IL-6 Trans-Signaling Activation in Deep Vein Thrombosis (DVT) Patients Biomolecules, 2022-05-10;12(5):. 2022-05-10 [PMID: 35625609] (Human)
• J Nordeng, H Schandiz, S Solheim, S Åkra, P Hoffman, B Roald, B Bendz, H Arnesen, R Helseth, I Seljeflot The Inflammasome Signaling Pathway Is Actively Regulated and Related to Myocardial Damage in Coronary Thrombi from Patients with STEMI Mediators of Inflammation, 2021-05-27;2021(0):5525917. 2021-05-27 [PMID: 34135690] (Human)
• Koji Osuka Inhibitory Mechanism of the Outer Membrane Growth of Chronic Subdural Hematomas J. Neurotrauma, 2017-02-27;0(0):. 2017-02-27 [PMID: 28027695] (Human)
• Holmlund U, Amoudruz P, Johansson MA, Haileselassie Y, Ongoiba A, Kayentao K, Traore B, Doumbo S, Schollin J, Doumbo O, Montgomery SM, Sverremark-Ekstrom E Maternal country of origin, breast milk characteristics and potential influences on immunity in offspring. Clin. Exp. Immunol., 2010-10-05;162(3):500-9. 2010-10-05 [PMID: 20942805] (Human)
• Nikolajuk A, Kowalska I, Karczewska-Kupczewska M, Adamska A, Otziomek E, Wolczynski S, Kinalska I, Gorska M, Straczkowski M Serum soluble glycoprotein 130 concentration is inversely related to insulin sensitivity in women with polycystic ovary syndrome. Diabetes, 2010-01-26;59(4):1026-9. 2010-01-26 [PMID: 20103703] (Human)
• Perez-Andres M, Almeida J, Martin-Ayuso M, De Las Heras N, Moro MJ, Martin-Nunez G, Galende J, Cuello R, Abuin I, Moreno I, Dominguez M, Hernandez J, Mateo G, San Miguel JF, Orfao A Soluble and membrane levels of molecules involved in the interaction between clonal plasma cells and the immunological microenvironment in multiple myeloma and their association with the characteristics of the disease. Int. J. Cancer, 2009-01-15;124(2):367-75. 2009-01-15 [PMID: 19003959] (Human)
• Richards PJ, Nowell MA, Horiuchi S, McLoughlin RM, Fielding CA, Grau S, Yamamoto N, Ehrmann M, Rose-John S, Williams AS, Topley N, Jones SA Functional characterization of a soluble gp130 isoform and its therapeutic capacity in an experimental model of inflammatory arthritis. Arthritis Rheum., 2006-05-01;54(5):1662-72. 2006-05-01 [PMID: 16646038] (Human)
• Hirota H, Izumi M, Hamaguchi T, Sugiyama S, Murakami E, Kunisada K, Fujio Y, Oshima Y, Nakaoka Y, Yamauchi-Takihara K Circulating interleukin-6 family cytokines and their receptors in patients with congestive heart failure. Heart Vessels, 2004-09-01;19(5):237-41. 2004-09-01 [PMID: 15372299] (Human)
• Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation. J. Virol., 2003-02-01;77(3):1727-37. 2003-02-01 [PMID: 12525606] (Primate - Macaca mulatta (Rhesus Macaque))
• Erices A, Conget P, Rojas C, Minguell JJ Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells. Exp. Cell Res., 2002-10-15;280(1):24-32. 2002-10-15 [PMID: 12372336] (Human)
• Hampel H, Teipel SJ, Padberg F, Haslinger A, Riemenschneider M, Schwarz MJ, Kotter HU, Scheloske M, Buch K, Stubner S, Dukoff R, Lasser R, Moller N, Sunderland T, Rapoport SI, Moller HJ Discriminant power of combined cerebrospinal fluid tau protein and of the soluble interleukin-6 receptor complex in the diagnosis of Alzheimer's disease. Brain Res., 1999-03-27;823(1):104-12. 1999-03-27 [PMID: 10095017] (Human)
• Stubner S, Schon T, Padberg F, Teipel SJ, Schwarz MJ, Haslinger A, Buch K, Dukoff R, Lasser R, Moller N, Sunderland T, Rapoport SI, Moller HJ, Hampel H Interleukin-6 and the soluble IL-6 receptor are decreased in cerebrospinal fluid of geriatric patients with major depression: no alteration of soluble gp130. Neurosci. Lett., 1999-01-15;259(3):145-8. 1999-01-15 [PMID: 10025579] (Human)

FAQs for gp130/CD130 (DGP00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL6ST