Recombinant Human IL‑33 induces IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC) in the presence of 0.25 ng/mL Recombinant Human IL-12 (Catalog # 219-IL) in a dose-dependent manner (orange ...read more
Simple Western lane view shows lysates of human kidney tissue and human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ST2/IL-33 R at approximately 60 kDa (as indicated) using 2 µg/mL of Goat ...read more
KG‑1a human acute myelogenous leukemia cell line was stained with Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523, filled histogram) or isotype control antibody (Catalog # ...read more
ST2/IL-33 R was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523) at 1.7 µg/mL for 1 hour at ...read more
Western blot shows lysates of human kidney tissue and human lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-HumanST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523) ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for ST2/IL-33R Antibody [Unconjugated]
interleukin 1 receptor-like 1
interleukin 1 receptor-related protein
interleukin-1 receptor-like 1
T1homolog of mouse growth stimulation-expressed
ST2, also known as IL-33R, IL-1 R4 and T1, is an
Interleukin-1 receptor family glycoprotein that contributes to Th2 immune
responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular
domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a
207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate
splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks
the transmembrane and cytoplasmic regions as well as an isoform that
additionally lacks the third Ig‑like domain (4). Within the ECD,
human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2,
respectively. ST2 is expressed on the surface of mast cells, activated Th2
cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine
that is upregulated by inflammation or mechanical strain in smooth muscle
cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33
binding induces the association of ST2 with IL-1R AcP, a shared signaling
subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In
macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by
sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in
promoting mast cell and Th2 dependent inflammation, ST2 activation enhances
antigen induced hypernociception and protects from atherosclerosis and cardiac
hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated
Th2 cells and strained cardiac myocytes and is elevated in the serum in
allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that
blocks IL‑33's ability
to signal through transmembrane ST2 (10, 13‑15).
Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
Tominaga, S. et al. (1992) Biochim. Biophys. Acta 1171:215.
Li, H. et al. (2000) Genomics 67:284.
Schmitz, J. et al. (2005) Immunity 23:479.
Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
Weinberg, E.O. et al. (2002) Circulation 106:2961.
Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
Palmer, G. et al. (2008) Cytokine 42:358.
Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.