ST2/IL-33R Antibody [Unconjugated]


Recombinant Human IL‑33 induces IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC) in the presence of 0.25 ng/mL Recombinant Human IL-12 (Catalog # 219-IL) in a dose-dependent manner (orange more
Simple Western lane view shows lysates of human kidney tissue and human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ST2/IL-33 R at approximately 60 kDa (as indicated) using 2 µg/mL of Goat more
KG‑1a human acute myelogenous leukemia cell line was stained with Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523, filled histogram) or isotype control antibody (Catalog # more
ST2/IL-33 R was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523) at 1.7 µg/mL for 1 hour at more
Western blot shows lysates of human kidney tissue and human lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-HumanST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523) more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, B/N, CyTOF-ready

Order Details

ST2/IL-33R Antibody [Unconjugated] Summary

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ST2/IL-33 R
Accession # BAA02233
Detects human ST2/IL-33 R in direct ELISAs and Western blots.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.2 ug/mL
  • Simple Western 2 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1-15 ug/mL
  • CyTOF-ready
  • Neutralization 0.1-0.6 ug/mL
Read Publications using
AF523 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST2/IL-33R Antibody [Unconjugated]

  • DER4
  • DER4ST2growth stimulation-expressed
  • Fit-1
  • IL-1 R4
  • IL1R4
  • IL-1R4
  • IL-1RL1
  • IL-33 R
  • IL33R
  • IL-33R
  • interleukin 1 receptor-like 1
  • interleukin 1 receptor-related protein
  • interleukin-1 receptor-like 1
  • Ly84
  • MGC32623
  • Protein ST2
  • ST2
  • ST2L
  • ST2V
  • T1
  • T1homolog of mouse growth stimulation-expressed


ST2, also known as IL-33R, IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks IL‑33's ability to signal through transmembrane ST2 (10, 13‑15).
  1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
  3. Tominaga, S. et al. (1992) Biochim. Biophys. Acta 1171:215.
  4. Li, H. et al. (2000) Genomics 67:284.
  5. Schmitz, J. et al. (2005) Immunity 23:479.
  6. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
  7. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
  8. Weinberg, E.O. et al. (2002) Circulation 106:2961.
  9. Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
  10. Palmer, G. et al. (2008) Cytokine 42:358.
  11. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  12. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
  13. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
  14. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
  15. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ST2/IL-33R Antibody (AF523)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Cell Culture, IHC-P, Neutralization, Western Blot.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF523 Applications Species
Q Zhou, X Wu, X Wang, Z Yu, T Pan, Z Li, X Chang, Z Jin, J Li, Z Zhu, B Liu, L Su The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-alpha/IL-33/ST2L signaling promotes gastric cancer metastasis Oncogene, 2019;0(0):. 2019 [PMID: 31659258] (Cell Culture, Human) Cell Culture Human
N Schaunaman, A Sanchez, KG Dimasuay, N Pavelka, M Numata, R Alam, RJ Martin, HW Chu Interleukin 1 Receptor-Like 1 (IL1RL1) Promotes Airway Bacterial and Viral Infection and Inflammation Infect. Immun., 2019;87(7):. 2019 [PMID: 31061143] (Western Blot, Human) Western Blot Human
WI Ryu, H Lee, HC Bae, J Jeon, HJ Ryu, J Kim, JH Kim, JW Son, J Kim, Y Imai, K Yamanishi, SH Jeong, SW Son IL-33 down-regulates CLDN1 expression through the ERK/STAT3 pathway in keratinocytes J. Dermatol. Sci., 2018;0(0):. 2018 [PMID: 29534857] (Western Blot, Human) Western Blot Human
Tanabe T, Shimokawaji T, Kanoh S, Rubin B IL-33 stimulates CXCL8/IL-8 secretion in goblet cells but not normally differentiated airway cells. Clin Exp Allergy, 2014;44(4):540-52. 2014 [PMID: 24479526] (Western Blot, Human) Western Blot Human
Southcombe J, Ledee N, Perrier d'Hauterive S, Turner K, Child T, Snider J, Redman C, Sargent I, Granne I Detection of soluble ST2 in human follicular fluid and luteinized granulosa cells. PLoS ONE, 2013;8(9):e74385. 2013 [PMID: 24040238] (Western Blot, Human) Western Blot Human
Granne I, Southcombe JH, Snider JV ST2 and IL-33 in pregnancy and pre-eclampsia. PLoS ONE, 2011;6(9):e24463. 2011 [PMID: 21949719] (IHC-P, Human) IHC-P Human
Hong YS, Moon S, Moon SJ, Joo YB, Joo Y, Jeon C, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis. J Korean Med Sci, 0;26(9):1132-9. 0 [PMID: 21935266] (IHC-P, Human) IHC-P Human
Nishida A, Andoh A, Imaeda H, Inatomi O, Shiomi H, Fujiyama Y Expression of interleukin 1-like cytokine interleukin 33 and its receptor complex (ST2L and IL1RAcP) in human pancreatic myofibroblasts. Gut, 2010;59(4):531-41. 2010 [PMID: 19996325] (Western Blot, Human) Western Blot Human
Cherry WB, Yoon J, Bartemes KR, Iijima K, Kita H A novel IL-1 family cytokine, IL-33, potently activates human eosinophils. J. Allergy Clin. Immunol., 2008;121(6):1484-90. 2008 [PMID: 18539196] (Neutralization, Human) Neutralization Human
Wade KC, Guttentag SH, Gonzales LW, Maschhoff KL, Gonzales J, Kolla V, Singhal S, Ballard PL Gene induction during differentiation of human pulmonary type II cells in vitro. Am. J. Respir. Cell Mol. Biol., 2006;34(6):727-37. 2006 [PMID: 16474099] (Western Blot, Human) Western Blot Human
Show All 11 Publications.

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Secondary Antibodies


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Other Available Formats

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Phycoerythrin FAB5231P

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Gene Symbol IL1RL1