Recombinant Human Insulin R/CD220 (aa 28-944) Protein

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Summary
Reactivity HuSpecies Glossary
Applications Binding Activity

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Recombinant Human Insulin R/CD220 (aa 28-944) Protein Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When 15 ng/mL of biotinylated recombinant human Insulin is added to serially diluted Recombinant Human Insulin R/CD220, the concentration of Recombinant Human Insulin R/CD220 that produces 50% of the optimal binding response is 0.03‑0.15 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Insulin R/CD220 protein
His28-Arg750 ( alpha subunit) & Ser751-Lys944 with a C-terminal 10-His tag ( beta subunit)
Accession #
N-terminal Sequence
His28 ( alpha subunit) & Ser751 ( beta subunit)
Structure / Form
Tetramer; disulfide-linked homodimer of disulfide-linked heterodimers ( alpha & beta )
Protein/Peptide Type
Recombinant Proteins
Gene
INSR
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
82.9 kDa ( alpha subunit), 22.9 kDa ( beta subunit).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
122-135 kDa and 33-43 kDa, reducing conditions
Publications
Read Publications using
1544-IR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Insulin R/CD220 (aa 28-944) Protein

  • CD 220
  • CD220 antigen
  • CD220
  • EC 2.7.10
  • EC 2.7.10.1
  • HHF5
  • INSR
  • Insulin R
  • insulin receptor
  • InsulinR
  • IR

Background

The Insulin Receptor (gene name INSR, designated CD220) is a type I transmembrane glycoprotein in the Insulin/IGF Receptor family of receptor tyrosine kinases that share structural similarity and overlapping intracellular signaling events (1-3). The 1370 amino acid (aa) human Insulin R preproprotein (A isoform) is processed by proteolysis to remove the signal peptide and produce an extracellular alpha portion (aa 28-750), and an extracellular/transmembrane/cytoplasmic beta subunit (aa 751-1370) (4). The extracellular domain (ECD) contains two homologous globular domains separated by a cysteine-rich domain and followed by three fibronectin type III domains. The intracellular region contains insulin-receptor substrate (IRS) docking sites, the kinase domain, and a phosphotyrosine-containing linker region. The human Insulin R ECD shares 96% aa sequence identity with mouse, rat, equine and canine Insulin R. As a result of alternative splicing, two INSR isoforms that differ by the absence (IR-A) or presence (IR-B) of a 12 aa residue sequence in the carboxyl terminus of the alpha subunit exist (4). IR-A expression is highest in fetal tissues and cancer cells, while IR-B is concentrated in adult differentiated cells (2-5). IR-A and IR-B may homodimerize, or heterodimerize with the IGF-I receptor (1, 3, 4). All receptor combinations bind insulin, IGF-I or IGF-II, but with differing affinities; for example, IR-A has considerably higher affinity for IGF-II as compared to IR-B (2-5). This system allows fine tuning of signaling pathways according to the concentrations of insulin, IGF-I and IGF-II, and expression of receptor subunits on the cell surface (2, 3). Insulin R signaling regulates glucose uptake and metabolism, but also contributes to cell growth, differentiation and apoptosis (2, 3, 5, 6). Mutations in the Insulin R gene have been linked severe insulin resistance (type A and Rabson-Mendenhall syndrome) that may include type II diabetes mellitus and, rarely, leprechaunism (Donohue syndrome) that also includes growth delays and endocrine system abnormalities (1, 7). The R&D Systems human Insulin R consists of the entire ECD of the IR-A isoform.

  1. Nakae, J. et al. (2001) Endoc. Rev. 22:818.
  2. Sciacca, L. et al. (2003) Endocrinology 144:2650.
  3. Belfiore, A. et al. (2009) Endocrine Rev. 30:586.
  4. Lawrence, M.C. et al. (2007) Curr. Opin. Struct. Biol. 17:699.
  5. Sacco, A. et al. (2009) Endocrinology 150:3594.
  6. Kitamura, T. et al. (2004) J. Clin. Invest. 113:209.
  7. Musso, C. et al. (2004) Medicine (Baltimore) 83:209.

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Publications for Insulin R/CD220 (1544-IR)(4)

We have publications tested in 2 confirmed species: Hamster, N/A.

We have publications tested in 2 applications: Bioassay, ELISA (Standard).


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Bioassay
(3)
ELISA (Standard)
(1)
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Hamster
(1)
N/A
(2)
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Showing Publications 1 - 4 of 4.
Publications using 1544-IR Applications Species
G Scapin, VP Dandey, Z Zhang, W Prosise, A Hruza, T Kelly, T Mayhood, C Strickland, CS Potter, B Carragher Structure of the Insulin Receptor-Insulin Complex by Single Particle CryoEM analysis Nature, 2018;0(0):. 2018 [PMID: 29512653] (Bioassay) Bioassay
S Mukherjee, M Chattopadh, S Bhattachar, S Dasgupta, S Hussain, SK Bharadwaj, D Talukdar, A Usmani, BS Pradhan, SS Majumdar, P Chattopadh, S Mukhopadhy, TK Maity, MK Chaudhuri, S Bhattachar A Small Insulinomimetic Molecule Also Improves Insulin Sensitivity in Diabetic Mice PLoS ONE, 2017;12(1):e0169809. 2017 [PMID: 28072841] (Bioassay, N/A) Bioassay N/A
Corbin, John A, Bhaskar, Vinay, Goldfine, Ira D, Issafras, Hassan, Bedinger, Daniel H, Lau, Angela, Michelson, Kristen, Gross, Lisa M, Maddux, Betty A, Kuan, Hua F, Tran, Catarina, Lao, Llewelyn, Handa, Masahisa, Watson, Susan R, Narasimha, Ajay J, Zhu, Shirley, Levy, Raphael, Webster, Lynn, Wijesuriya, Sujeewa, Liu, Naichi, Wu, Xiaorong, Chemla-Vogel, David, Lee, Steve R, Wong, Steve, Wilcock, Diane, Rubin, Paul, White, Mark L Inhibition of insulin receptor function by a human, allosteric monoclonal antibody: a potential new approach for the treatment of hyperinsulinemic hypoglycemia. MAbs, 2014;6(1):262-72. 2014 [PMID: 24423625] (Bioassay, Hamster) Bioassay Hamster
Umehara A, Nishioka M, Obata T A novel ultra-sensitive enzyme immunoassay for soluble human insulin receptor ectodomain and its measurement in urine from healthy subjects and patients with diabetes mellitus. Clin. Biochem., 2009;42(13):1468-75. 2009 [PMID: 19560451] (ELISA (Standard), N/A) ELISA (Standard) N/A

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Bioinformatics

Gene Symbol INSR
Uniprot