IL-33 Antibody [Unconjugated]

Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with full length mouse IL-33. PVDF membrane was probed with 0.4 µg/mL of Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-33 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL-33 was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

IL-33 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

bEND.3 cells were stained with Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Mouse splenocytes were treated for 5 hours with 50 ng/mL PMA and 1 µg/mL Ca2+ionomycin then stained with Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Recombinant Mouse IL-33 (Catalog # 3626-ML) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-33 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626). The ND50 is typically 10-50 ng/mL.

IL-33/ST2 axis in tendon healing in vivo.Kinetics of (a) Il33 and (b) soluble St2 gene expression post injury. Data are mean fold change±s.d. showing relative expression to 18s housekeeping gene (pooled data from four mice per group performed on four sequential occasions, n=16 per condition). *P<0.05, **P<0.01 versus control (WT) mice (ANOVA) +P<0.05,++P<0.01, NS not significant; control (WT) injured versus St2−/− injured. (c) Immunohistochemistry showing IL-33 and ST2 expression in tendon biopsies of WT and St2−/− mice day 1 post injury. IgG control shown in bottom left of pictures. Black horizontal line indicates 50 μm. (d) Col3 mRNA and (e) Collagen 3 protein levels in tendon biopsies of WT and St2−/− mice post injury. (f) Col1 mRNA and (g) Collagen 1 protein levels in tendon biopsies of WT and St2−/− mice post injury. For (d–g) data are mean±s.d. of duplicate samples, representative of four mice per condition (n=16). *P<0.05, **P<0.01 versus control (WT) mice. +P<0.05, ++P<0.01 control (WT) injured versus St2−/− injured mice (ANOVA). mRNA graphs show relative expression to 18s housekeeping gene. (h) Percentage change in tendon strength for WT and St2−/− mice post injury. Data are mean±s.d., representative of four mice per condition (n=16). *P<0.05, **P<0.01 versus control mice. #P<0.05 St2−/− injured versus WT injured mice (Mann–Whitney U-test). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25857925), licensed under a CC-BY license. Not internally tested by R&D Systems.

HMGB1 increases IL-33 expression and acts as an autocrine factor in enhancing IL-33 expression in a partial TLR-4 dependent fashion during cyclic stretch.(A) MLE-12 cells were treated with 3 μg/ml HMGB1 or control solution before 6h cyclic stretch and IL-33 production was measured by ELISA. (B) MLE-12 cells were treated with 10 μg/ml HMGB1 neutralizing antibody (2G7) or control solution before 6h cyclic stretch and IL-33 production was detected by Western blot. beta -actin served as loading control. (C) MLE-12 cells transfecting with non-specific control siRNA or TLR-4-specific siRNA were treated with 3 μg/ml HMGB1 or control solution before stretch. IL-33 production in each group was measured with ELISA. **P<0.01, ***P<0.001 when compared between groups denoted by horizontal lines. Each stretching group collected at least from three wells and represented a single experiment, the bar graphs illustrate data representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28898270), licensed under a CC-BY license. Not internally tested by R&D Systems.

IL-33/ST2 expression in human tendon.(a) IL-33, (b) soluble ST2 (sST2) and (c) mST2 mRNA expression in human tendon samples. Fold change in gene expression of IL-33, sST2 and mST2 in control (semi-membranosus tendon, n=10), torn supraspinatus tendon (established pathology) and matched subscapularis human tendon samples (early pathology; n=17). Data are mean±s.d. relative to the housekeeping gene18S (mean of duplicate analysis). *P<0.05, **P<0.01, ***P<0.001 versus control (Student's t-test). (d) Immunostaining of IL-33 and ST2 in control (n=10), torn tendon (n=17) and early tendinopathy (n=17). Graphs illustrate modified Bonar scoring based on 10 high-power fields. Data are mean±s.d. *P<0.05, **P<0.01 versus control (Student's t-test). Scale bar, 65 μm. (e) Fold change in gene expression of IL33 and ST2 24 h post incubation with tumour necrosis factor (TNF-alpha ), IL-1 beta or in combination depicting relative expression to media alone utilizing housekeeping gene GAPDH. Data are mean±s.d. of triplicate samples, representative of three individual patient samples. *P<0.05, **P<0.01 versus control (media) (Student's t-test). (f) IL-33 immunostaining of human tendon explants cultured for 24 h with medium (control), 100 ng ml−1 TNF alpha or 100 ng ml−1 TNF alpha +100 ng ml−1 IL-1 beta . (g) Fold change in COL1 and COL3 mRNA expression in human tendon explants cultured for 24 h with rhIL-33, relative to housekeeping gene GAPDH. (h) Time course of COL1 and COL3 mRNA expression following incubation with rhIL-33, relative to housekeeping gene GAPDH. (i) Collagen 1 and 3 protein expression in human tendon explants 24 h post incubation with rhIL-33. For (g–i) data are mean±s.d. of triplicate samples, representative of three individual patients. *P<0.05, **P<0.01 versus control (Student's t-test). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25857925), licensed under a CC-BY license. Not internally tested by R&D Systems.

Prior Nb exposure results in earlier and exacerbated HSV-2 pathology. (I and J) (I) Vaginal IL-33 measured by ELISA (day 2) and (J) IF staining of vaginal tissue (day 3; n = 4). Data are representative of two independent experiments with 4–6 mice per group (mean ± SEM). Statistical significance was calculated by two-way analysis of variance (ANOVA) with Bonferroni correction for multiple comparisons and Mann-Whitney t test. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ns, not significant. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33857419), licensed under a CC-BY license. Not internally tested by R&D Systems.

IL13 expression in FAPs is upregulated in Tchp−/− mice. A) qRT‐PCR analysis of RNA isolated from whole TA muscle of WT and Tchp−/− mice (n = 3 or 4) at 3 dpi. B) Immunofluorescence for IL13 (red), hematopoietic cells (CD45, green), and nuclei (Hoechst33342, blue) in TA muscle cross‐sections of WT and Tchp−/− mice at 3 dpi. Lower magnification images are shown in Figure S6A, Supporting Information. Scale bars, 10 µm. C) Quantification of the IL4‐, IL13‐, or IL33‐stained area per 40× view in (B) and Figure S6A,B (Supporting Information) (n = 6 or 7 fields each). D) Quantification of the IL13‐stained area per 40× view excluding the CD45‐stained area in (B) (n = 7 fields each). E) Immunofluorescence for IL4 (red), IL13 (red), and IL33 (red), PDGFR alpha + (green), and nuclei (Hoechst33342, blue) in PDGFR alpha + FAPs isolated from hindlimbs of WT and Tchp−/‐ mice. Scale bars: 20 µm. All data are the mean ± S.D from 3 to 5 mice. *p < 0.05, **p < 0.01, N.S., not significant; two‐tailed unpaired Student's t‐tests. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36373718), licensed under a CC-BY license. Not internally tested by R&D Systems.

IL13 expression in FAPs is upregulated in Tchp−/− mice. A) qRT‐PCR analysis of RNA isolated from whole TA muscle of WT and Tchp−/− mice (n = 3 or 4) at 3 dpi. B) Immunofluorescence for IL13 (red), hematopoietic cells (CD45, green), and nuclei (Hoechst33342, blue) in TA muscle cross‐sections of WT and Tchp−/− mice at 3 dpi. Lower magnification images are shown in Figure S6A, Supporting Information. Scale bars, 10 µm. C) Quantification of the IL4‐, IL13‐, or IL33‐stained area per 40× view in (B) and Figure S6A,B (Supporting Information) (n = 6 or 7 fields each). D) Quantification of the IL13‐stained area per 40× view excluding the CD45‐stained area in (B) (n = 7 fields each). E) Immunofluorescence for IL4 (red), IL13 (red), and IL33 (red), PDGFR alpha + (green), and nuclei (Hoechst33342, blue) in PDGFR alpha + FAPs isolated from hindlimbs of WT and Tchp−/‐ mice. Scale bars: 20 µm. All data are the mean ± S.D from 3 to 5 mice. *p < 0.05, **p < 0.01, N.S., not significant; two‐tailed unpaired Student's t‐tests. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36373718), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, IHC, B/N, CyTOF-ready, ELISA(Cap), Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-33 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL-33
  Ser109-Ile266
  Accession # Q8BVZ5
• Specificity: Detects mouse IL-33 in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant human IL‑33 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL33
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 10-50 ng/mL
  • Western Blot 0.4 ug/mL
• Application Notes: ELISA Capture: Mouse IL-33 Antibody (Catalog # AF3626)
  ELISA Detection:Mouse IL-33 Biotinylated Antibody (Catalog # BAF3626)
  Standard: Recombinant Mouse IL-33 (Catalog # 3626-ML)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-33 Antibody [Unconjugated]

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% aa sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
3. Schmitz, J. et al. (2005) Immunity 23:479.
4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
7. Dinarello, C.A. (2005) Immunity 23:461.
8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-33 Antibody (AF3626)(148)

Publications using AF3626

• Woo, JH;Song, MK;Baek, SH;Park, CJ;Kim, JW;Kim, B;Lee, K; Polystyrene particles induces asthma-like Th2-mediated lung injury through IL-33 secretion Environment international 2025-09-08 [PMID: 40926454] (Western Blot, Mouse)
• Arvind, V;Crosio, G;Howell, K;Zhang, H;Montero, A;Huang, AH; Functional tendon regeneration is driven by regulatory T cells and IL-33 signaling Science advances 2025-04-25 [PMID: 40267206] (Immunohistochemistry, Transgenic Mouse)
• Sanchez, NC;Mani, G;Nowak, JI;Jones, C;Kim, YI;Sharma, NS;Zmijewski, JW;Surolia, R; Persistent neutrophilic inflammation is associated with delayed toxicity of phenylarsine oxide in lungs Scientific reports 2025-04-07 [PMID: 40195488] (Immunohistochemistry, Mouse)
• Ikehata, Y;Oshima, E;Hayashi, Y;Tanaka, Y;Sato, H;Hitomi, S;Shiratori-Hayashi, M;Urata, K;Kimura, Y;Shibuta, I;Ohba, S;Iwata, K;Mizuta, K;Shirota, T;Shinoda, M; Fibroblast-derived IL-33 exacerbates orofacial neuropathic pain via the activation of TRPA1 in trigeminal ganglion neurons Brain, behavior, and immunity 2024-11-03 [PMID: 39500418] (Immunohistochemistry, Western Blot, Mouse)
• Wang, M;Dufort, C;Du, Z;Shi, R;Xu, F;Huang, Z;Sigler, AR;Leak, RK;Hu, X; IL-33/ST2 signaling in monocyte-derived macrophages maintains blood-brain barrier integrity and restricts infarctions early after ischemic stroke Journal of neuroinflammation 2024-10-24 [PMID: 39449077] (Immunohistochemistry, Mouse)
• Asadollahi, E;Trevisiol, A;Saab, AS;Looser, ZJ;Dibaj, P;Ebrahimi, R;Kusch, K;Ruhwedel, T;Möbius, W;Jahn, O;Lee, JY;Don, AS;Khalil, MA;Hiller, K;Baes, M;Weber, B;Abel, ED;Ballabio, A;Popko, B;Kassmann, CM;Ehrenreich, H;Hirrlinger, J;Nave, KA; Oligodendroglial fatty acid metabolism as a central nervous system energy reserve Nature neuroscience 2024-09-09 [PMID: 39251890] (Immunohistochemistry, Mouse)
• Bisen, S;Verma, SK;Mukhopadhyay, CS;Singh, NK; A neutrophil elastase-generated mature form of IL-33 is a potent regulator of endothelial cell activation and proliferative retinopathy Experimental & molecular medicine 2024-08-01 [PMID: 39085349] (Immunoprecipitation, Mouse)
• Jiao, M;Wang, C;Tang, X;Dai, C;Zhang, N;Fan, A;Qian, Z;Liu, S;Zhang, F;Li, B;Xu, Y;Tan, Z;Gong, F;Lu, Y;Zheng, F; Active secretion of IL-33 from astrocytes is dependent on TMED10 and promotes central nervous system homeostasis Brain, behavior, and immunity 2024-04-24 [PMID: 38663774] (Western Blot, Neutralization, Mouse, Transgenic Mouse)
• Wei, H;Zhen, L;Wang, S;Yang, L;Zhang, S;Zhang, Y;Jia, P;Wang, T;Wang, K;Zhang, Y;Ma, L;Lv, J;Zhang, P; Glyceryl triacetate promotes blood-brain barrier recovery after ischemic stroke through lipogenesis-mediated IL-33 in mice Journal of neuroinflammation 2023-11-15 [PMID: 37968698] (IHC, Western Blot, Mouse)
• Hassan, D;Chen, J; CEBPA restricts alveolar type 2 cell plasticity during development and injury-repair bioRxiv : the preprint server for biology 2023-10-12 [PMID: 37873326] (IHC, Mouse)
• Yaling Jiang, Valentina Donati, Godefridus J. Peters, Elisa Giovannetti, Dong Mei Deng Fungal mycobiome-mediated immune response: a non-negligible promoter in pancreatic oncogenesis and chemoresistance Cancer Drug Resistance 2023-05-10 [PMID: 37457135]
• Du, J;Liu, Y;Lan, G;Zhou, Y;Ni, Y;Liao, K;Zheng, F;Cheng, Q;Shi, G;Su, X; PTRF-IL33-ZBP1 signaling mediating macrophage necroptosis contributes to HDM-induced airway inflammation Cell death & disease 2023-07-15 [PMID: 37454215] (Western Blot, Mouse)
• Geetika Kaur, Deepti Sharma, Shivantika Bisen, Chandra Sekhar Mukhopadhyay, Katherine Gurdziel, Nikhlesh K. Singh Vascular cell-adhesion molecule 1 (VCAM-1) regulates JunB-mediated IL-8/CXCL1 expression and pathological neovascularization Communications Biology 2023-05-13 [PMID: 37179352]
• Z Ma, W Li, L Zhuang, T Wen, P Wang, H Yu, Y Liu, Y Yu TMEM59 ablation leads to loss of olfactory sensory neurons and impairs olfactory functions via interaction with inflammation Brain, Behavior, and Immunity, 2023-04-13;111(0):151-168. 2023-04-13 [PMID: 37061103] (IHC, Moyse)
• M Liu, J Huang, T Liu, J Yuan, C Lv, Z Sha, C Wu, W Jiang, X Liu, M Nie, Y Chen, S Dong, Y Qian, C Gao, Y Fan, D Wu, R Jiang Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice Acta neuropathologica communications, 2023-04-07;11(1):61. 2023-04-07 [PMID: 37024941] (In Vivo, Mouse)
• AM Benvie, D Lee, BM Steiner, S Xue, Y Jiang, DC Berry Age-dependent Pdgfrbeta signaling drives adipocyte progenitor dysfunction to alter the beige adipogenic niche in male mice Nature Communications, 2023-03-31;14(1):1806. 2023-03-31 [PMID: 37002214] (Neutralization, Mouse)
• J Hou, Y Zhou, Z Cai, M Terekhova, A Swain, PS Andhey, RM Guimaraes, A Ulezko Ant, T Qiu, S Sviben, G Strout, JAJ Fitzpatric, Y Chen, S Gilfillan, DH Kim, SJ Van Dyken, MN Artyomov, M Colonna Transcriptomic atlas and interaction networks of brain cells in mouse CNS demyelination and remyelination Cell Reports, 2023-03-21;42(4):112293. 2023-03-21 [PMID: 36952346] (IHC, Mouse)
• Xuyue Zhou, Yu Hu, Lingxi Liu, Lihao Liu, Hongying Chen, Dan Huang et al. IL-33-mediated activation of mast cells is involved in the progression of imiquimod-induced psoriasis-like dermatitis Cell Communication and Signaling 2023-03-09 [PMID: 36894987] (Immunohistochemistry, Human, Mouse)
• Cheong LY, Wang B, Wang Q et al. Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice Nature communications 2023-03-03 [PMID: 36869026]
• Zhao J, Jung S, Li X et al. Delivery of costimulatory blockade to lymph nodes promotes transplant acceptance in mice Journal of Clinical Investigation 2022-12-15 [PMID: 36519543]
• Alessandra Gurtner, Costanza Borrelli, Ignacio Gonzalez-Perez, Karsten Bach, Ilhan E. Acar, Nicolás G. Núñez et al. Active eosinophils regulate host defence and immune responses in colitis Nature 2023-03-02 [PMID: 36509106]
• Lotte Vanherle, Darcy Lidington, Franziska E. Uhl, Saskia Steiner, Stefania Vassallo, Cecilia Skoug et al. Restoring myocardial infarction-induced long-term memory impairment by targeting the cystic fibrosis transmembrane regulator eBioMedicine 2022-12-01 [PMID: 36462404]
• S Li, H Liu, Y Qian, L Jiang, S Liu, Y Liu, C Liu, X Gu IL-33/ST2 axis promotes remodeling of the extracellular matrix and drives protective microglial responses in the mouse model of perioperative neurocognitive disorders International immunopharmacology, 2022-11-26;114(0):109479. 2022-11-26 [PMID: 36446234] (IHC, Western Blot, Mouse)
• Yamakawa D, Tsuboi J, Kasahara K et al. Cilia-Mediated Insulin/Akt and ST2/JNK Signaling Pathways Regulate the Recovery of Muscle Injury Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11/14/2022 [PMID: 36373718]
  
  Details:
  Dilution used in WB 1:1000
• L Xu, C Wei, Y Chen, Y Wu, X Shou, W Chen, D Lu, H Sun, W Li, B Yu, X Wang, X Zhang, Y Yu, Z Lei, R Tang, J Zhu, Y Li, L Lu, H Zhou, S Zhou, C Su, X Chen IL-33 induces thymic involution-associated naive T cell aging and impairs host control of severe infection Nature Communications, 2022-11-12;13(1):6881. 2022-11-12 [PMID: 36371464] (Neutralization, Western Blot, Mouse)
• Ajay Dixit, Aaron L Sarver, Jon Zettervall, Huocong Huang, Kexin Zheng, Rolf A. Brekken et al. Targeting TNF-alpha producing macrophages activate antitumor immunity in pancreatic cancer through IL33 signaling JCI Insight 2022-11-22 [PMID: 36256464]
• GM Jowett, E Read, LB Roberts, D Coman, M Vilà Gonzá, T Zabinski, U Niazi, R Reis, TJ Trieu, D Danovi, E Gentleman, L Vallier, MA Curtis, GM Lord, JF Neves Organoids capture tissue-specific innate lymphoid cell development in mice and humans Cell Reports, 2022-08-30;40(9):111281. 2022-08-30 [PMID: 36044863] (Neutralization, Mouse)
• Darine W. El-Naccache, Fei Chen, Mark J. Palma, Alexander Lemenze, Matthew A. Fischer, Wenhui Wu et al. Adenosine metabolized from extracellular ATP promotes type 2 immunity through triggering A2BAR signaling in intestinal epithelial cells Cell Reports 2022-08-02 [PMID: 35926464]
• J Glaubitz, A Wilden, J Golchert, G Homuth, U Völker, BM Bröker, T Thiele, MM Lerch, J Mayerle, AA Aghdassi, FU Weiss, M Sendler In mouse chronic pancreatitis CD25+FOXP3+ regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response Nature Communications, 2022-08-03;13(1):4502. 2022-08-03 [PMID: 35922425] (Flow Cytometry, Mouse)
• P Widmayer, P Pregitzer, H Breer Short-term high fat feeding induces inflammatory responses of tuft cells and mucosal barrier cells in the murine stomach Histology and histopathology, 2022-07-29;0(0):18503. 2022-07-29 [PMID: 35904321] (IHC, Mouse)
• Deepti Sharma, Shivantika Bisen, Geetika Kaur, Eric C. Van Buren, Gadiparthi N. Rao, Nikhlesh K. Singh IL-33 enhances Jagged1 mediated NOTCH1 intracellular domain (NICD) deubiquitination and pathological angiogenesis in proliferative retinopathy Communications Biology 2022-05-19 [PMID: 35589941]
• Xue Li, Bo Jiang, Yu Zou, Jie Zhang, Yuan-Yuan Fu, Xiao-Yue Zhai Roxadustat (FG-4592) Facilitates Recovery From Renal Damage by Ameliorating Mitochondrial Dysfunction Induced by Folic Acid Frontiers in Pharmacology 2022-02-25 [PMID: 35280255] (Western Blot, Mouse)
• P Deng, L Wang, Q Zhang, S Chen, Y Zhang, H Xu, H Chen, Y Xu, W He, J Zhang, H Sun Therapeutic Potential of a Combination of Electroacupuncture and Human iPSC-Derived Small Extracellular Vesicles for Ischemic Stroke Cells, 2022-02-26;11(5):. 2022-02-26 [PMID: 35269441] (Flow Cytometry, Western Blot, Mouse)
• K Kos, MA Aslam, R van de Ven, MD Wellenstei, W Pieters, A van Weverw, DEM Duits, K van Pul, CS Hau, K Vrijland, D Kaldenbach, EAM Raeven, SA Quezada, R Beyaert, H Jacobs, TD de Gruijl, KE de Visser Tumor-educated Tregs drive organ-specific metastasis in breast cancer by impairing NK cells in the lymph node niche Cell Reports, 2022-03-01;38(9):110447. 2022-03-01 [PMID: 35235800] (Neutralization, Mouse)
• Y Xie, AT Kuan, W Wang, ZT Herbert, O Mosto, O Olukoya, M Adam, S Vu, M Kim, D Tran, N Gómez, C Charpentie, I Sorour, TE Lacey, MY Tolstoruko, BL Sabatini, WA Lee, CC Harwell Astrocyte-neuron crosstalk through Hedgehog signaling mediates cortical synapse development Cell Reports, 2022-02-22;38(8):110416. 2/22/2022 [PMID: 35196485]
• Aftab Alam, Eric Levanduski, Parker Denz, Helena Solleiro Villavicencio, Maulasri Bhatta, Lamees Alhorebi et al. Fungal mycobiome drives IL-33 secretion and type 2 immunity in pancreatic cancer Cancer Cell 2022-02-01 [PMID: 35120601]
• Meng X, Qian X, Ding X et al. Eosinophils regulate intra-adipose axonal plasticity Proceedings of the National Academy of Sciences of the United States of America 1/18/2022 [PMID: 35042776]
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Reviews for IL-33 Antibody (AF3626) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Chao Xiong IHC-Fr Mouse 03/14/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: 4% PFA-PBS fixed frozen sections from murine brain
• Species: Mouse
• Lot: YJE0717041

reviewed by: Verified Customer ICC Mouse 01/08/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 23172891
• Species: Mouse

Bioinformatics

• Gene Symbol: IL33
• Uniprot:
  • Q8BVZ5()