ST2/IL-33R Antibodies

HAF017). A specific band was detected for ST2/IL-33 R at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for ST2/IL-33 R at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human kidney tissue and human lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for ST2/IL-33 R at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

219-IL) in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (DIF50C). Under these conditions, IFN-gamma secretion elicited by IL-33 is neutralized (green line) by increasing concentrations of Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523). The ND₅₀ is typically 0.1-0.6 µg/mL." class="big_lightbox"> 219-IL) in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (DIF50C). Under these conditions, IFN-gamma secretion elicited by IL-33 is neutralized (green line) by increasing concentrations of Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523). The ND₅₀ is typically 0.1-0.6 µg/mL." class="big_thumb" />

MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113)." class="big_lightbox"> MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113)." alt="P815 mouse mastocytoma cell line was stained with Rat Anti-Mouse ST2/IL-33 R Monoclonal Antibody (Catalog # MAB10041, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113)." class="big_thumb" />

AR002). Under these conditions, proliferation elicited by IL-33 is neutralized (green line) by increasing concentrations of Rat Anti-Mouse ST2/IL-33 R Monoclonal Antibody (Catalog # MAB10041). The ND₅₀ is typically 0.5-3 µg/mL." class="big_lightbox"> AR002). Under these conditions, proliferation elicited by IL-33 is neutralized (green line) by increasing concentrations of Rat Anti-Mouse ST2/IL-33 R Monoclonal Antibody (Catalog # MAB10041). The ND₅₀ is typically 0.5-3 µg/mL." class="big_thumb" />

1004-MR) was serially diluted and captured by Rat Anti-Mouse ST2/IL-33R Monoclonal Antibody (Catalog # MAB10041) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ST2/IL-33R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1004) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1004-MR) was serially diluted and captured by Rat Anti-Mouse ST2/IL-33R Monoclonal Antibody (Catalog # MAB10041) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ST2/IL-33R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1004) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

3625-IL) induces proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL-33 is neutralized (green line) by increasing concentrations of Goat Anti-Mouse ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1004). The ND₅₀ is typically 1-6 µg/mL." class="big_lightbox"> 3625-IL) induces proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL-33 is neutralized (green line) by increasing concentrations of Goat Anti-Mouse ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1004). The ND₅₀ is typically 1-6 µg/mL." alt="Recombinant Human IL-33 (Catalog # 3625-IL) induces proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL-33 is neutralized (green line) by increasing concentrations of Goat Anti-Mouse ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1004). The ND₅₀ is typically 1-6 µg/mL." class="big_thumb" />

1004-MR) was serially diluted and captured by Rat Anti-Mouse ST2/IL-33R Monoclonal Antibody (Catalog # MAB10041) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ST2/IL-33R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1004) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1004-MR) was serially diluted and captured by Rat Anti-Mouse ST2/IL-33R Monoclonal Antibody (Catalog # MAB10041) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ST2/IL-33R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1004) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

219-IL) in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by IL-33 is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate ST2/IL-33 R Monoclonal Antibody (Catalog # MAB523). The ND₅₀ is typically 0.3-1.5 µg/mL." class="big_lightbox"> 219-IL) in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by IL-33 is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate ST2/IL-33 R Monoclonal Antibody (Catalog # MAB523). The ND₅₀ is typically 0.3-1.5 µg/mL." alt="Recombinant Human IL-33 induces IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC) in the presence of Recombinant Human IL-12 (Catalog # 219-IL) in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by IL-33 is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate ST2/IL-33 R Monoclonal Antibody (Catalog # MAB523). The ND₅₀ is typically 0.3-1.5 µg/mL." class="big_thumb" />

HAF008). Specific bands were detected for ST2/IL-33 R at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). Specific bands were detected for ST2/IL-33 R at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human lung tissue and human placenta tissue. PVDF membrane was probed with 5 µg/mL of Rabbit Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB10118) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for ST2/IL-33 R at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />