SRC1 Antibody

Images

 
Biological Strategies: Western Blot: SRC1 Antibody [NB100-312] - Detection of Human and Mouse SRC1 by Western Blot. Samples: Whole cell lysate from 293T (50, 15, 5 ug), and (50 ug) from HeLa, and mouse NIH3T3 ...read more
Immunohistochemistry: SRC1 Antibody [NB100-312] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SRC1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC ...read more
Western Blot: SRC1 Antibody [NB100-312] - Nuclear extract (10 mg) from HeLa cells. Antibody used at 20 ug/10 mg extract.
Immunoprecipitation: SRC1 Antibody [NB100-312] - Detection of human SRC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

SRC1 Antibody Summary

Immunogen
A 29 amino acid synthetic peptide, which represented a portion of human nuclear receptor coactivator 1 encoded within exon 12 (LocusLink ID 8648).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCOA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot 1:1000-1:10000
Application Notes
NB 100-312 may be used for Western blot. The investigator should determine the optimal working dilution for a specific application.
Control
HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB500-160, NBP1-19102

Alternate Names for SRC1 Antibody

  • bHLHe42
  • BHLHE74
  • Class E basic helix-loop-helix protein 74
  • EC 2.3.1.48
  • Hin-2 protein
  • KAT13A
  • MGC129719
  • NCoA-1
  • nuclear receptor coactivator 1
  • PAX3/NCOA1 fusion protein
  • Protein Hin-2
  • Renal carcinoma antigen NY-REN-52
  • RIP160bHLHe74F-SRC-1
  • SRC-1
  • SRC1MGC129720
  • Steroid receptor coactivator 1
  • steroid receptor coactivator-1

Background

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-1 (SRC 1), a 110 kDa protein. SRC 1 has been shown to interact with and stimulate the transcriptional activity of estrogen, progesterone, retinoic acid, thyroid and glucocorticoid receptors, as well as retinoic X receptor (RXR) in a ligand dependent manner. The amino terminal region of SRC 1 contains a PAS-A-basic helix-loop-helix homology domain which has previously been shown to be dimerization domains in other nuclear transcription factors including the aryl hydrocarbon (Ah) receptor and its heterodimerization partner, ARNT. The carboxy-terminal region of SRC 1 has been shown to possess histone acetyltransferase activity specific primarily for histones H3 & H4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NCOA1