SRC1 Antibody - BSA Free

Western Blot: SRC1 Antibody [NB100-312] - Detection of Human and Mouse SRC1 by Western Blot. Samples: Whole cell lysate from 293T (50, 15, 5 ug), and (50 ug) from HeLa, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC1 antibody NB100-312 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: SRC1 Antibody [NB100-312] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SRC1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Western Blot: SRC1 Antibody [NB100-312] - Nuclear extract (10 mg) from HeLa cells. Antibody used at 20 ug/10 mg extract.

Immunoprecipitation: SRC1 Antibody [NB100-312] - Detection of human SRC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SRC1 antibody NB100-312 (lot NB100-312-2) used for IP at 6 ug per reaction. SRC1 was also immunoprecipitated by a previous lot of this antibody NB100-312-1. For blotting immunoprecipitated SRC1, NB100-312 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SRC1 Antibody - BSA Free Summary

• Description: This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB500-160, NBP1-19102
• Immunogen: A 29 amino acid synthetic peptide, which represented a portion of human nuclear receptor coactivator 1 encoded within exon 12 (LocusLink ID 8648).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCOA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot 1:1000-1:10000
• Application Notes: NB 100-312 may be used for Western blot. The investigator should determine the optimal working dilution for a specific application.
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB500-160, NBP1-19102

Alternate Names for SRC1 Antibody - BSA Free

• bHLHe42
• bHLHe74
• Class E basic helix-loop-helix protein 74
• EC 2.3.1.48
• Hin-2 protein
• KAT13A
• MGC129719
• NCOA1
• NCoA-1
• nuclear receptor coactivator 1
• PAX3/NCOA1 fusion protein
• Protein Hin-2
• Renal carcinoma antigen NY-REN-52
• RIP160
• RIP160bHLHe74F-SRC-1
• SRC1
• SRC-1
• SRC1MGC129720
• Steroid receptor coactivator 1
• steroid receptor coactivator-1

Background

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-1 (SRC 1), a 110 kDa protein. SRC 1 has been shown to interact with and stimulate the transcriptional activity of estrogen, progesterone, retinoic acid, thyroid and glucocorticoid receptors, as well as retinoic X receptor (RXR) in a ligand dependent manner. The amino terminal region of SRC 1 contains a PAS-A-basic helix-loop-helix homology domain which has previously been shown to be dimerization domains in other nuclear transcription factors including the aryl hydrocarbon (Ah) receptor and its heterodimerization partner, ARNT. The carboxy-terminal region of SRC 1 has been shown to possess histone acetyltransferase activity specific primarily for histones H3 & H4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCOA1