PELP1 Antibody

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Western Blot: PELP1 Antibody [NB200-331] - Detection of mouse PELP1/MNAR by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331] - Human ovarian carcinoma.~ Antibodies: Affinity purified rabbit anti-PELP1/MNAR used at a dilution of 1:2,500 (0.4ug/ml) (left) used at a dilution of 1:5,000 ...read more
Biological Strategies: Western Blot: PELP1 Antibody [NB200-331] - Detection of Human PELP1/MNAR by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. ...read more
Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331] - Samples: FFPE sections of mouse renal cell carcinoma.~ Antibodies: Affinity purified rabbit anti-PELP1/MNAR dilution of 1:2,500 (0.4ug/ml) (left) and used at ...read more
Independent Antibodies: Immunoprecipitation: PELP1 Antibody [NB200-331] - Detection of human PELP1/MNAR by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PELP1 Antibody Summary

Immunogen
A synthetic peptide mapping to a region between residues 1000 and 1050 of human, proline-, glutamic acid-, leucine-rich protein 1/ modulator of nongenomic activity of estrogen receptor using the numbering given in entry AAN41255.1 (GeneID 27043).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PELP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:2000 - 1:10000
  • Immunohistochemistry-Paraffin 1:2000 - 1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2500-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Control
HeLa Nuclear Cell Lysate
Publications
Read Publications using
NB200-331 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PELP1 Antibody

  • HMX3
  • MNARmodulator of nongenomic activity of estrogen receptor
  • Modulator of non-genomic activity of estrogen receptor
  • P160
  • proline and glutamic acid rich nuclear protein
  • proline, glutamate and leucine rich protein 1
  • proline, glutamic acid and leucine rich protein 1
  • proline-, glutamic acid- and leucine-rich protein 1
  • proline-, glutamic acid-, leucine-rich protein 1
  • Transcription factor HMX3

Background

PELP1 is a coactivator of estrogen receptor (see ESR1; MIM 133430)-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors (Choi et al., 2004).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PELP1 Antibody (NB200-331)(18)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Filter By Species
Human
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB200-331 Applications Species
Regan Anderson TM, Ma SH, Raj GV et al. Breast Tumor Kinase (Brk/PTK6) Is Induced by HIF, Glucocorticoid Receptor, and PELP1-Mediated Stress Signaling in Triple-Negative Breast Cancer Cancer Res. Jan 29 2016 [PMID: 26825173] (WB, Human) WB Human
Cortez V, Mann M, Tekmal S et al. Targeting the PELP1-KDM1 axis as a potential therapeutic strategy for breast cancer. Breast Cancer Res 2012 Jul [PMID: 22812534]
Chakravarty D, Roy SS, Babu CR et al. Therapeutic targeting of PELP1 prevents ovarian cancer growth and metastasis. Clin Cancer Res 2011 Apr [PMID: 21421858]
Nair BC, Nair SS, Chakravarty D et al. Cyclin-dependent kinase-mediated phosphorylation plays a critical role in the oncogenic functions of PELP1. Cancer Res 2010 Sep [PMID: 20807815]
Kayahara M, Ohanian J, Ohanian V et al. MNAR functionally interacts with both NH2- and COOH-terminal GR domains to modulate transactivation. Am J Physiol Endocrinol Metab 2008 Nov [PMID: 18682536]
Dimple C, Nair SS, Rajhans R et al. Role of PELP1/MNAR signaling in ovarian tumorigenesis. Cancer Res 2008 Jun [PMID: 18559538]
Nagpal JK, Nair S, Chakravarty D et al. Growth factor regulation of estrogen receptor coregulator PELP1 functions via Protein Kinase A pathway. Mol Cancer Res 2008 May [PMID: 18505929]
Galluzzo P, Ascenzi P, Bulzomi P et al. The nutritional flavanone naringenin triggers antiestrogenic effects by regulating estrogen receptor alpha-palmitoylation. Endocrinology 2008 May [PMID: 18239068]
Rajhans R, Nair HB, Nair SS et al. Modulation of in situ estrogen synthesis by proline-, glutamic acid-, and leucine-rich protein-1: potential estrogen receptor autocrine signaling loop in breast cancer cells. Mol Endocrinol 2008 Mar [PMID: 18079323]
Ohshiro K, Rayala SK, Kondo S et al. Identifying the estrogen receptor coactivator PELP1 in autophagosomes. Cancer Res 2007 Sep [PMID: 17804729]
Show All 18 Publications.

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PELP1
Entrez
Uniprot