SR-AI/MSR Antibody - BSA Free

Western Blot: SR-AI/MSR Antibody [NBP1-00093] - Detection of SR-AI/MSR in human liver.

Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody [NBP1-00093] - The SR-AI/MSR antibody was tested in Raw cells at a 1:50 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Eq, Pm
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SR-AI/MSR Antibody - BSA Free Summary

• Immunogen: Synthetic peptide made to a cyclized peptide from human Macrophage Scavenger Receptor I (within residues 400-450). [Swiss-Prot# P21757]
• Localization: Membrane; Single-pass type II membrane protein.
• Predicted Species: Rat (92%), Canine (100%), Equine (100%), Primate (93%), Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MSR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 0.5 ug/ml
• Application Notes: This Macrophage Scavenger Receptor I antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~50 kDa.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)
  SR-AI/MSR Overexpression Lysate

• Control Peptide:

  SR-AI/MSR Peptide (NBP1-00093PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SR-AI/MSR Antibody - BSA Free

• CD204 antigen
• CD204
• Macrophage acetylated LDL receptor I and II
• macrophage scavenger receptor 1
• macrophage scavenger receptor type III
• MSR1
• phSR1
• phSR2
• SCARA1
• SCARA1macrophage scavenger receptor types I and II
• Scavenger receptor class A member 1
• scavenger receptor class A, member 1
• SR-A
• SRAI
• SR-AI

Background

Macrophage Scavenger Receptor I (MSR1, also called CD204 or scavenger receptor A) belongs to scavenger receptor A family and represents phagocytic pattern-recognition receptors (PRRs) expressed predominantly on macrophages and dendritic cells. MSR1 is a single-pass type II membrane glycoprotein capable of binding to a wide range of polyanionic ligands (e.g., modified low density lipoproteins, pathogen-derived molecules), and is implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis, innate immune response as well as Alzheimer's disease. MSR1 mediates the endocytosis of a diverse group of macromolecules including modified LDLs, and scavenger receptors MSR1, CD36 and LOX-1 are three main receptors for oxidized LDL on macrophage membrane wherein MSR1/CD36 account for more than 9/10 of the lipid accumulation in macrophages exposed to oxidized LDL. Defects in MSR1 have also been linked to prostate cancer and Barrett esophagus (BE).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MSR1