RNase L Antibody (2E9)


Western Blot: RNase L Antibody (2E9) [NB100-351] - RNase L detected in insect cell extracts transfected with recombinant human RNase L. 1 and 0.15 ug of extract.
Immunohistochemistry: RNase L Antibody (2E9) [NB100-351] - RNase L antibody was tested in human colon using DAB with hematoxylin counterstain.
Western Blot: RNase L Antibody (2E9) [NB100-351] - RNaseL in transfected cells (0.1 ug) and Hey1B (100 ug)

Product Details

Reactivity Hu, Ha, Pm, Mu(-), Rt(-)Species Glossary
Applications WB, ELISA, IHC, IHC-P
1.7 mg/ml

RNase L Antibody (2E9) Summary

Purified full-length recombinant human RNaseL produced in insect cells. [UniProt# Q05823]
Cytoplasmic and Mitochondrial.
IgG1 Kappa
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1% Sodium Azide
1.7 mg/ml
Protein G purified


Application Notes
This RNase L (2E9) antibody is useful for ELISA, Western Blot and Immunohistochemistry on paraffin-embedded sections. By WB, this antibody recognizes a band at ~83 kDa, representing RNase L. A low-molecular mass, 37 kDa, isoform of RNase L has been described in peripheral blood mononuclear cell extracts. In IHC-P, staining was observed in the cytoplasm of human colon cells. Prior to immunostaining paraffin tissues, antigen retrieval with Tris-EDTA buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
83 kDa
Read Publications using
NB100-351 in the following applications:

  • WB
    2 publications

Reactivity Notes

Human, hamster and primate. This antibody does not react with mouse or rat.

Alternate Names for RNase L Antibody (2E9)

  • 2-5A-dependent ribonuclease
  • 2-5A-dependent RNase
  • 2'-5'-oligoisoadenylate synthetase-dependent
  • EC 3.1.26.-
  • HPC1
  • interferon-induced 2-5A-dependent RNase
  • MGC104972
  • MGC133329
  • PRCA1
  • prostate cancer 1
  • Ribonuclease 4
  • ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent)
  • Ribonuclease L
  • RNase L
  • RNS4DKFZp781D08126


A virally induced endoribonuclease, RNase L is the terminal factor of the anti-viral action of interferon. Activation of RNase L results in inhibition of viral proliferation. Activation of RNase L has also been determined to degrade RNA, thus, initiating a cellular stress response, inducing apoptosis. Since mutations in RNase L relate to an increased incidence of prostate cancer, the activation of RNase L functions in counteracting prostate cancer, via apoptosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RNase L Antibody (NB100-351)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.

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Showing Publications 1 - 9 of 9.
Publications using NB100-351 Applications Species
Al-Haj L, Blackshear PJ, Khabar KS. Regulation of p21/CIP1/WAF-1 mediated cell-cycle arrest by RNase L and tristetraprolin, and involvement of AU-rich elements Nucleic Acids Res 2012 Jun 19 [PMID:22718976] (WB, Human) WB Human
Fabre O, Salehzada T, Lambert K et al. RNase L controls terminal adipocyte differentiation, lipids storage and insulin sensitivity via CHOP10 mRNA regulation. Cell Death Differ 2012 [PMID:22441668]
Lin RJ, Yu HP, Chang BL et al. Distinct antiviral roles for human 2',5'-oligoadenylate synthetase family members against dengue virus infection. J Immunol;183(12):8035-43. 2009 Dec 15. [PMID:19923450]
Al-Ahmadi, W et al. Alternative polyadenylation variants of the RNA binding protein, HuR: abundance, role of AU-rich elements and auto-Regulation. Nucleic Acids Res;37(11):3612-24. 2009 Jun. [PMID:19359363]
Han, JQ et al. A phylogenetically conserved RNA structure in the poliovirus open reading frame inhibits the antiviral endoribonuclease RNase L. J Virol;81(11):5561-72. 2007 Jun. [PMID:17344297]
Carpten, J et al. Germline mutations in the ribonuclease L gene in families showing linkage with HPC1. Nat Genet;30(2):181-4. 2002 Feb. [PMID:11799394]
Wang, L et al. Elevated levels of 2',5'-linked oligoadenylate-dependent ribonuclease L occur as an early event in colorectal tumorigenesis. Clin Cancer Res;1(11):1421-8. 1995 Nov. [PMID:9815940]
Dong, B et al. A bipartite model of 2-5A-dependent RNase L. J Biol Chem;272(35):22236-42. 1997 Aug 29. [PMID:9268370] (WB, Human) WB Human
Dong, B et al. 2-5A-dependent RNase molecules dimerize during activation by 2-5A. J Biol Chem;270(8):4133-7. 1995 Feb 24. [PMID:7876164]

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-351AF405
Alexa Fluor (R) 488 Labeled NB100-351AF488
Alexa Fluor (R) 647 Labeled NB100-351AF647
Alexa Fluor (R) 700 Labeled NB100-351AF700
Biotin Labeled NB100-351B
DyLight 405 Labeled NB100-351V
DyLight 488 Labeled NB100-351G
DyLight 550 Labeled NB100-351R
DyLight 650 Labeled NB100-351C
FITC Labeled NB100-351F
HRP Labeled NB100-351H

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