RNase L Antibody (2E9) - BSA Free

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Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351] - Analysis for RNase L in WT and eIF2a-S51A MEF cell lines transduced with RNase L-encoding lentivirus. n.s. indicates nonspecific band for the purpose of ...read more
Immunohistochemistry: RNase L Antibody (2E9) [NB100-351] - RNase L antibody was tested in human colon using DAB with hematoxylin counterstain.
Western Blot: RNase L Antibody (2E9) [NB100-351] - RNaseL in insect cell extracts transfected with recombinant human RNase L (0.1 ug) and Hey1B (100 ug)
Genetic Strategies: Knockout Validated: RNase L Antibody (2E9) - BSA Free [NB100-351] - Analysis of PKR and RNase L in parental (WT), RNase L-KO (RL-KO), PKR-KO, and RNase L/PKR double knockout (RL/PKR-KO) A549 ...read more
HPV-16E7 and -58E7 upregulated CIP2A mRNA and protein levels in PHKs(A) Western blot analysis of CIP2A protein level in PHKs expressing HPV-16E7, -58E7, -6E7; and (B) Quantification. (C) qRT-PCR analysis of CIP2A mRNA ...read more
Spy1 stable protein induces anchorage independent growth. (A) 293 cells were transfected with different concentrations of Myc-Spy1-WT, Myc-Spy1-TST or 30 μg of empty PCS3 vector control. Soft agar assay was carried out ...read more

Product Details

Summary
Reactivity Hu, Ha, Pm, Mu(-), Rt(-)Species Glossary
Applications WB, ELISA, IHC, KO
Clone
2E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

RNase L Antibody (2E9) - BSA Free Summary

Immunogen
Purified full-length recombinant human RNaseL produced in insect cells. [UniProt# Q05823]
Localization
Cytoplasmic and Mitochondrial.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RNASEL
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Knockout Validated
  • Western Blot 1:1000-1:4000 (ECL)
Application Notes
This RNase L (2E9) antibody is useful for ELISA, Western Blot and Immunohistochemistry on paraffin-embedded sections. By WB, this antibody recognizes a band at ~83 kDa, representing RNase L. A low-molecular mass, 37 kDa, isoform of RNase L has been described in peripheral blood mononuclear cell extracts. In IHC-P, staining was observed in the cytoplasm of human colon cells. Prior to immunostaining paraffin tissues, antigen retrieval with Tris-EDTA buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-351 in the following applications:

  • WB
    5 publications

Reactivity Notes

This antibody does not react with mouse or rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for RNase L Antibody (2E9) - BSA Free

  • 2-5A-dependent ribonuclease
  • 2-5A-dependent RNase
  • 2'-5'-oligoisoadenylate synthetase-dependent
  • EC 3.1.26.-
  • HPC1
  • interferon-induced 2-5A-dependent RNase
  • MGC104972
  • MGC133329
  • PRCA1
  • prostate cancer 1
  • Ribonuclease 4
  • ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent)
  • Ribonuclease L
  • RNase L
  • RNS4DKFZp781D08126

Background

A virally induced endoribonuclease, RNase L is the terminal factor of the anti-viral action of interferon. Activation of RNase L results in inhibition of viral proliferation. Activation of RNase L has also been determined to degrade RNA, thus, initiating a cellular stress response, inducing apoptosis. Since mutations in RNase L relate to an increased incidence of prostate cancer, the activation of RNase L functions in counteracting prostate cancer, via apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, IHC, KO

Publications for RNase L Antibody (NB100-351)(14)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(5)
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Filter By Species
Human
(4)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB100-351 Applications Species
Burke JM, Ripin N, Ferretti MB et al. RNase L activation in the cytoplasm induces aberrant processing of mRNAs in the nucleus PLoS pathogens 2022-11-01 [PMID: 36318584] (WB, Human)

Details:
Dilution used in WB 1:1500
WB Human
Corbet GA, Burke JM, Bublitz GR, Parker R dsRNA-induced condensation of antiviral proteins promotes PKR activation J Biol Chem 2020-01-04 [PMID: 31896577] (WB, Human) WB Human
Burke JM, Moon SL, Matheny T, Parker R RNase L Reprograms Translation by Widespread mRNA Turnover Escaped by Antiviral mRNAs Mol. Cell [PMID: 31494035]
Al-Haj L, Blackshear PJ, Khabar KS. Regulation of p21/CIP1/WAF-1 mediated cell-cycle arrest by RNase L and tristetraprolin, and involvement of AU-rich elements Nucleic Acids Res 2012-06-19 [PMID: 22718976] (WB, Human) WB Human
Fabre O, Salehzada T, Lambert K et al. RNase L controls terminal adipocyte differentiation, lipids storage and insulin sensitivity via CHOP10 mRNA regulation. Cell Death Differ 2012-01-01 [PMID: 22441668]
Lin RJ, Yu HP, Chang BL et al. Distinct antiviral roles for human 2',5'-oligoadenylate synthetase family members against dengue virus infection. J Immunol;183(12):8035-43. 2009-12-15 [PMID: 19923450]
Al-Ahmadi, W et al. Alternative polyadenylation variants of the RNA binding protein, HuR: abundance, role of AU-rich elements and auto-Regulation. Nucleic Acids Res;37(11):3612-24. 2009-06-01 [PMID: 19359363]
Han, JQ et al. A phylogenetically conserved RNA structure in the poliovirus open reading frame inhibits the antiviral endoribonuclease RNase L. J Virol;81(11):5561-72. 2007-06-01 [PMID: 17344297]
Carpten, J et al. Germline mutations in the ribonuclease L gene in families showing linkage with HPC1. Nat Genet;30(2):181-4. 2002-02-01 [PMID: 11799394]
Wang, L et al. Elevated levels of 2',5'-linked oligoadenylate-dependent ribonuclease L occur as an early event in colorectal tumorigenesis. Clin Cancer Res;1(11):1421-8. 1995-11-01 [PMID: 9815940]
Show All 14 Publications.

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Bioinformatics

Gene Symbol RNASEL