Sphingomyelin Synthase 2 Antibody - Azide and BSA Free

Western Blot: Sphingomyelin Synthase 2 Antibody [NBP3-30889] - Fig 1. Western blot on human brain lysate (10 ug/lane) using Novus Biologicals' NBP3-30889 antibody to Sphingomyelin Synthase 2 (0.5 ug/ml). Lane A] 1 ug/ml antibody alone, lane B] antibody plus 3 ug blocking peptide. Blot was developed with Pierce’s Super System West Femto - 1 minute exposure. Fig 2. Human kidney section stained with Novus Biologicals' anti-SMS-2 antibody (Cat NBP3-30889) at 2 ug/ml. Anti-rabbit Ig H+L HRP conjugate (5 ug/ml) without antigen retrieval.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Sphingomyelin Synthase 2 Antibody - Azide and BSA Free Summary

• Immunogen: Synthetic peptide derived from the human Sphingomyelin Synthase 2 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SGMS2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry : 1:400
  • Western Blot : 1:400
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline, pH 7.4
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Sphingomyelin Synthase 2 Antibody - Azide and BSA Free

• EC 2.7.8.27
• phosphatidylcholine:ceramide cholinephosphotransferase 2
• SM synthase
• sphingomyelin synthase 2SMS2MGC26963

Background

Sphingomyelin (SM) is a major component of plasma membranes. It is preferentially concentrated in the outer leaflet and has a role in the formation of lipid rafts. SM synthases (EC 2.7.8.27), such as SGMS2, produce SM in the lumen of the Golgi and on the cell surface through the transfer of phosphocholine from phosphatidylcholine onto ceramide, yielding diacylglycerol as a side product (Huitema et al., 2004 [PubMed 14685263]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SGMS2