UGCG Antibody (1E5)

Western Blot: UGCG Antibody (1E5) [H00007357-M03] - Analysis of UGCG expression in HL-60.

Western Blot: UGCG Antibody (1E5) [H00007357-M03] - Detection of UGCG in HT29 human colon carcinoma cells. Image provided courtesy of Dr. James Madigan.

ELISA: UGCG Antibody (1E5.) [H00007357-M03] - UGCG Antibody (1E5) [H00007357-M03] - Detection limit for recombinant GST tagged UGCG is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

UGCG Antibody (1E5) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: UGCG (NP_003349, 33 a.a. ~ 131 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TRLHLNKKATDKQPYSKLPGVSLLKPLKGVDPNLINNLETFFELDYPKYEVLLCVQDHDDPAIDVCKKLLGKYPNVDARLFIGGKKVGINPKINNLMPG
• Specificity: Reacts with UDP-glucose ceramide glucosyltransferase.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: UGCG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UGCG Antibody (1E5)

• ceramide glucosyltransferase
• EC 2.4.1.80
• GCS
• GCSGLCT1
• GLCT-1
• Glucosylceramide synthase
• UDP-glucose ceramide glucosyltransferaseUDP-glucose:N-acylsphingosine D-glucosyltransferase
• UGCG

Background

Glycosphingolipids (GSLs) are a group of membrane components that contain lipid and sugar moieties. They are present in essentially all animal cells and are believed to have important roles in various cellular processes. UDP-glucose ceramide glucosyltransferase catalyzes the first glycosylation step in glycosphingolipid biosynthesis. The product, glucosylceramide, is the core structure of more than 300 GSLs. UGCG is widely expressed and transciption is upregulated during keratinocyte differentiation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UGCG Antibody (H00007357-M03)(4)

Publications using H00007357-M03

• Drews K, Calgi M, Harrison W et al. Glucosylceramide synthase maintains influenza virus entry and infection. PLoS One. 2020-02-07 [PMID: 32032363]
• Drake M. J, Brennan B, et al. A role for glycolipid biosynthesis in severe fever with thrombocytopenia syndrome virus entry. PLoS Pathog 2017-04-01 [PMID: 28388693]
• Shibina A, Seidel D, Somanchi SS et al. Fenretinide sensitizes multidrug-resistant human neuroblastoma cells to antibody-independent and ch14.18-mediated NK cell cytotoxicity. J Mol Med (Berl). 2012-09-30 [PMID: 23052481]
• Haynes TA, Filippov V, Filippova M et al. DNA damage induces down-regulation of UDP-glucose ceramide glucosyltransferase, increases ceramide levels and triggers apoptosis in p53-deficient cancer cells. Biochim Biophys Acta. 2012-02-11 [PMID: 22349266]

Review for UGCG Antibody (H00007357-M03) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 04/17/2010

Summary

• Application: Western Blot
• Sample Tested: HT29 human colorectal carcinoma cells, Sample Amount: 40ug
• Species: Human

Bioinformatics

• Gene Symbol: UGCG
• Entrez:
  • 7357(Human)
• Uniprot:
  • NP_003349(Human)