SMPD1 Antibody (OTI3H7)

Western blot shows culture media of U‑87 MG human glioblastoma/astrocytoma parental cell line and SMPD1 knockdown U-87 MG cell line (KD). Nitrocellulose membrane was probed with SMPD1 Antibody (OTI3H7) (Catalog # NBP2-45889) followed by HRP-conjugated secondary antibody. A specific band was detected for SMPD1 at approximately 69.9 kDa (as indicated) in the parental U-87 MG cell line, but is significantly reduced in the knockdown U-87 MG cell line. Primary antibody dilution used: 1/500. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Western Blot: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMPD1.

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

SMPD1 Antibody (OTI3H7)[NBP2-45889]Simple Western� analysis of endogenous protein RIOK2 from HELA lysates (0.2 mg/mL) using RIOK2 Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

• Reactivity: Hu
• Applications: WB, IHC, Mycoplasma
• Clone: OTI3H7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

SMPD1 Antibody (OTI3H7) Summary

• Description: Novus Biologicals Mouse SMPD1 Antibody (OTI3H7) (NBP2-45889) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-SMPD1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 62-341 of human SMPD1 (NP_000534) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMPD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Frozen Reported in scientific literature (PMID:32092464)
  • Immunohistochemistry-Paraffin 1:150
  • Knockdown Validated Knockdown validated from YCharOS Inc. (YCharOS.com)
  • Western Blot 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SMPD1 Antibody (OTI3H7)

• Acid sphingomyelinase
• aSMase
• ASMsphingomyelin phosphodiesterase
• EC 3.1.4.12
• NPD
• SMPD1
• sphingomyelin phosphodiesterase 1, acid lysosomal

Background

The protein encoded by the SMPD1 gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Three transcript variants encoding two different isoforms have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMPD1 Antibody (NBP2-45889)(4)

Publications using NBP2-45889

• van Smeden, J, Al-Khakany, H Et al. Skin barrier lipid enzyme activity in Netherton patients is associated with protease activity and ceramide abnormalities. J Lipid Res 2020-06-01 [PMID: 32265319] (FLOW, WB, Human)
• Rana S, Espinosa-Diez C, Ruhl R et al. Differential regulation of microRNA-15a by radiation affects angiogenesis and tumor growth via modulation of acid sphingomyelinase Sci Rep 2020-03-27 [PMID: 32221387] (WB, Human)
• Boer DEC, van Smeden J, Al-Khakany H et al. Skin of atopic dermatitis patients shows disturbed beta-glucocerebrosidase and acid sphingomyelinase activity that relates to changes in stratum corneum lipid composition Biochim Biophys Acta Mol Cell Biol Lipids 2020-02-21 [PMID: 32092464] (IHC-Fr, Human)
• Zhao W Acid Sphingomyelinase Deficiency Prevents AdipoRon-induced Transcription Factor EB activation and Differentiation in Arterial Smooth Muscle Cells Thesis 2022-01-01 (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: SMPD1