RAI1 Antibody - BSA Free

Immunohistochemistry-Paraffin: RAI1 Antibody [NBP1-28745] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RAI1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: RAI1 Antibody [NBP1-28745] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RAI1 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. RAI1 was also immunoprecipitated by rabbit anti-RAI1 antibody BL6549, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RAI1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RAI1 Antibody - BSA Free (NBP1-28745) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human retinoic acid induced 1 using the numbering given in entry NP_109590.3 (GeneID 10743).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RAI1 Antibody - BSA Free

• KIAA1820DKFZP434A139
• MGC12824
• retinoic acid induced 1
• retinoic acid-induced protein 1
• SMCR
• Smith-Magenis syndrome chromosome region
• SMSDKFZp434A139

Background

Defects in Retinoic acid-induced protein 1 (RAI1) are a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. SMS is characterized by congenital mental retardation associated with development and growth delays. The RAI1 has also been associated with schizophrenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAI1
• Entrez:
  • 10743(Human)
• Uniprot:
  • NP_109590.3(Human)