Spectrin beta 2 Antibody

Western Blot: Spectrin beta 2 Antibody [NB100-40829] - Detection of Human SPTBN1 on HeLa whole cell lysate using NB100-40829. SPTBN1 was also immunoprecipitated by rabbit anti-SPTBN1 antibody NB100-40828.

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NB100-40829] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SPTBN1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Spectrin beta 2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2150 and 2200 of human Spectrin Non-Erythroid Beta 1 using the numbering given in entry NP_003119.2 (GeneID 6711).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPTBN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Spectrin beta 2 Antibody

• beta-fodrin
• beta-G spectrin
• Beta-II spectrin
• beta-spectrin 2
• beta-spectrin II
• betaSpII
• ELF
• embryonic liver beta-fodrin
• Fodrin beta chain
• spectrin beta chain, brain 1
• spectrin, beta, non-erythrocytic 1
• Spectrin, non-erythroid beta chain 1
• SPTB2

Background

SPTBN1 is a major constituent of the erythrocyte skeleton, accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass. It is associated with the cytoplasmic surface of the membrane by attachment to ankyrin, a peripheral membrane protein. The membrane skeleton influences several cellular properties such as cell shape, restriction of mobility of the integral membrane protein exposed at the cell surface, and transmembrane movement of phospholipids and cholesterol. While the simplest form of SPTBN1 in solution is an alpha-beta heterodimer, SPTBN1 can undergo self-association in various conditions to form a tetramer of alpha2-beta2. In recent years a large number of SPTBN1-like molecules have been found in non-erythroid cells. These proteins are known by such different names as fodrin, CBP I, calspectin and TW 260/240.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Spectrin beta 2 Antibody (NB100-40829)(1)

Publication using NB100-40829

• Salcedo-Sicilia L, Granell S, Jovic M et al. betaIII spectrin regulates the structural integrity and the secretory protein transport of the Golgi complex J Biol Chem 2012-12-11 [PMID: 23233669] (IP, Human)

Bioinformatics

• Gene Symbol: SPTBN1
• Entrez:
  • 6711(Human)
• Uniprot:
  • Q01082(Human)