Spectrin beta 2 Antibody

Images

 
Independent Antibodies: Western Blot: Spectrin beta 2 Antibody [NB100-40829] - Detection of Human SPTBN1 on HeLa whole cell lysate using NB100-40829. SPTBN1 was also immunoprecipitated by rabbit anti-SPTBN1 ...read more
Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NB100-40829] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SPTBN1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Spectrin beta 2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 2150 and 2200 of human Spectrin Non-Erythroid Beta 1 using the numbering given in entry NP_003119.2 (GeneID 6711).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPTBN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Publications
Read Publication using
NB100-40829 in the following applications:

  • IP
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Spectrin beta 2 Antibody

  • beta-fodrin
  • beta-G spectrin
  • Beta-II spectrin
  • beta-spectrin 2
  • beta-spectrin II
  • betaSpII
  • ELF
  • embryonic liver beta-fodrin
  • Fodrin beta chain
  • spectrin beta chain, brain 1
  • spectrin, beta, non-erythrocytic 1
  • Spectrin, non-erythroid beta chain 1
  • SPTB2

Background

SPTBN1 is a major constituent of the erythrocyte skeleton, accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass. It is associated with the cytoplasmic surface of the membrane by attachment to ankyrin, a peripheral membrane protein. The membrane skeleton influences several cellular properties such as cell shape, restriction of mobility of the integral membrane protein exposed at the cell surface, and transmembrane movement of phospholipids and cholesterol. While the simplest form of SPTBN1 in solution is an alpha-beta heterodimer, SPTBN1 can undergo self-association in various conditions to form a tetramer of alpha2-beta2. In recent years a large number of SPTBN1-like molecules have been found in non-erythroid cells. These proteins are known by such different names as fodrin, CBP I, calspectin and TW 260/240.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Spectrin beta 2 Antibody (NB100-40829)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IP.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SPTBN1
Entrez
Uniprot