Spectrin beta 2 Antibody

Western Blot: Spectrin beta 2 Antibody [NB100-40828] - Detection of mouse SPTBN1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody NB100-40828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: Spectrin beta 2 Antibody [NB100-40828] - Detection of human SPTBN1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SPTBN1 (NB100-40828). Detection: DAB

Western Blot: Spectrin beta 2 Antibody [NB100-40828] - Detection of human SPTBN1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody NB100-40828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoprecipitation: Spectrin beta 2 Antibody [NB100-40828] - Detection of human Spectrin beta 2 (SPTBN1) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Spectrin beta 2 antibody NB100-40828 used for IP at 3 ug per reaction. SPTBN1 was also immunoprecipitated by rabbit anti-Spectrin beta 2 antibody NB100-40829. For blotting immunoprecipitated SPTBN1, NB100-40828 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Spectrin beta 2 Antibody Summary

• Description: Novus Biologicals Rabbit Spectrin beta 2 Antibody (NB100-40828) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2100 and 2150 of human Spectrin Non-Erythroid Beta 1 using the numbering given in entry NP_003119.2 (GeneID 6711).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPTBN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Spectrin beta 2 Antibody

• beta-fodrin
• beta-G spectrin
• Beta-II spectrin
• beta-spectrin 2
• beta-spectrin II
• betaSpII
• ELF
• embryonic liver beta-fodrin
• Fodrin beta chain
• spectrin beta chain, brain 1
• spectrin, beta, non-erythrocytic 1
• Spectrin, non-erythroid beta chain 1
• SPTB2

Background

SPTBN1 is a major constituent of the erythrocyte skeleton, accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass. It is associated with the cytoplasmic surface of the membrane by attachment to ankyrin, a peripheral membrane protein. The membrane skeleton influences several cellular properties such as cell shape, restriction of mobility of the integral membrane protein exposed at the cell surface, and transmembrane movement of phospholipids and cholesterol. While the simplest form of SPTBN1 in solution is an alpha-beta heterodimer, SPTBN1 can undergo self-association in various conditions to form a tetramer of alpha2-beta2. In recent years a large number of SPTBN1-like molecules have been found in non-erythroid cells. These proteins are known by such different names as fodrin, CBP I, calspectin and TW 260/240.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Spectrin beta 2 Antibody (NB100-40828). (Showing 1 - 1 of 1 FAQ).

1. We would like to know the concentration of the product NB100-40828.
   • NB100-40828 is supplied at a concentration of 0.2mg/ml.

Bioinformatics

• Gene Symbol: SPTBN1
• Entrez:
  • 6711(Human)
  • 20740(Mouse)
• Uniprot:
  • Q01082(Human)
  • Q62261(Mouse)