SOX4 Antibody (CL5665) - BSA Free

Immunocytochemistry/Immunofluorescence: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of SH-SY5Y cells using the Anti-SOX4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of human endometrium shows moderate to strong nuclear positivity in stromal cells.

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of human glioma shows moderate to strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of human liver shows absence of nuclear positivity in hepatocytes as expected (negative control).

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of mouse forebrain shows strong positivity in cells in the rostral migratory stream, as well as in a subset of neurons in the olfactory bulb.

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of mouse forebrain shows strong immunoreactivity in cells in the rostral migratory stream.

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of mouse hippocampus shows strong immunoreactivity in a subset of neurons in the granule cell layer of the dentate gyrus.

Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of rat hippocampus shows strong nuclear immunoreactivity in a subset of cells in the granule cell layer of the dentate gyrus.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clone: CL5665
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

SOX4 Antibody (CL5665) - BSA Free Summary

• Description: Novus Biologicals Mouse SOX4 Antibody (CL5665) - BSA Free (NBP2-61421) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from Q06945, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOX4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: PFA/Triton X-100

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for SOX4 Antibody (CL5665) - BSA Free

• ecotropic viral integration site 16
• EVI16
• SRY (sex determining region Y)-box 4
• SRY-related HMG-box gene 4
• transcription factor SOX-4

Background

SOX4 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOX4