SOX15 Antibody [Unconjugated]

Images

 
Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SOX15 Antigen Affinity-purified ...read more
SOX15 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Sheep Anti-Human SOX15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4070) at 10 µg/mL for 3 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

SOX15 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX15
Met1-Leu233
Accession # O60248
Specificity
Detects human SOX15 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) SOX3, rhSOX14, and rhSOX21 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
SOX15
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF4070 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX15 Antibody [Unconjugated]

  • protein SOX-15
  • SOX15
  • SOX20
  • SOX20Protein SOX-20
  • SOX26
  • SOX26SOX12
  • SOX27
  • SOX27Protein SOX-12
  • SRY (sex determining region Y)-box 15
  • SRY (sex determining region Y)-box 20

Background

SOX15 (also known as SOX20) is a nuclear, 25 kDa transcriptional repressor that belongs to group G of the SOX gene family. Human SOX15 is 233 amino acids (aa) in length and contains a 63 aa DNA-binding HMG-box (amino acids 48‑110). Although the C-terminus (aa 126‑233) does not bind DNA, it is required for SOX15 repressive activity. It may accomplish this by interacting with beta -catenin. SOX15 is expressed in myoblasts and regulates skeletal muscle regeneration and differentiation. Human SOX15 shares 75% aa sequence identity with mouse SOX15.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX15 Antibody (AF4070)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Secondary Antibodies

 

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Other Available Formats

Biotin BAF4070

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Bioinformatics

Gene Symbol SOX15
Uniprot