SOX5 Antibody

Western blot shows lysates of human lung tissue and exponentially growing LNCaP human prostate cancer cell line whole cell extract. PVDF membrane was probed with 1 µg/mL of Human SOX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5286) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOX5 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SOX5 Antibody Summary

• Immunogen: E. coli-derived recombinant human SOX5
  Met417-Asn763
  Accession # P35711
• Specificity: Detects endogenous human SOX5 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SOX5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX5 Antibody

• L-SOX5
• MGC35153
• SOX5
• SRY (sex determining region Y)-box 5
• transcription factor SOX-5

Background

SOX5 (SRY-related HMG box) is a 75-80 kDa Group D member of the SOX family of DNA-binding proteins. It is expressed in fetal chondrocytes and skeletal muscle, with a short form appearing in spermatids. SOX5 form homodimers and heterodimerizes with SOX6. Human SOX5 is 763 amino acids (aa) in length and contains two coiled-coil regions (aa 193-274 and 448-515), plus one Q/glutamine (aa 234-272) and one HMG box (aa 556-624). HMG domains participate in DNA-binding and protein-protein interaction. There are two alternate start sites at Met14 and Met417 (which creates a short/testis form), plus one variant with a three aa substitution for aa 389-763, another variant with a two aa substitution for aa 1-388, and a third variant with a one aa substitution for aa 534-763. Over aa 417-763, human SOX5 is 97% aa identical to mouse SOX5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOX5 Antibody (AF5286)(1)

Publication using AF5286

• Xin Chen, Qi Zheng, Weidong Li, Yuan Lu, Yiming Ni, Liang Ma et al. SOX5 induces lung adenocarcinoma angiogenesis by inducing the expression of VEGF through STAT3 signaling OncoTargets and Therapy 2018-09-27 [PMID: 30254466] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SOX5
• Uniprot:
  • P35711()