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SOX10 Antibody (SD204-04)

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Immunocytochemistry/Immunofluorescence: SOX10 Antibody (SD204-04) [NBP2-67812] - Staining SOX10 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (SD204-04) [NBP2-67812] - Staining SOX10 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SOX10 antibody. The section was pre-treated using heat mediated ...read more
Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (SD204-04) [NBP2-67812] - Staining SOX10 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Analysis of paraffin-embedded mouse brain tissue using anti-SOX10 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Analysis of paraffin-embedded rat brain tissue using anti-SOX10 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SOX10 antibody. The section was pre-treated using heat mediated ...read more
Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-SOX10 antibody. The section was pre-treated using heat mediated ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
SD204-04
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

100% Guarantee on every product

SOX10 Antibody (SD204-04) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within human SOX10 aa 410-450. (SwissProt: P56693 Human ;SwissProt: Q04888 Mouse ;SwissProt: O55170 Rat)
Localization
Cytoplasm, Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SOX10
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for SOX10 Antibody (SD204-04)

  • DOM
  • MGC15649
  • PCWH
  • SOX10
  • SRY (sex determining region Y)-box 10
  • SRY-related HMG-box gene 10
  • transcription factor SOX-10
  • WS4
  • WS4C
  • WS4mouse, human homolog of

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: MITF Antibody (21D1418) [NB100-56561] - Analysis of a FFPE tissue section of human skin using 1:200 dilution of MITF (21D1418) antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. General cytoplasmic staining appears enriched in melanocytes below the epidermis. Some nuclear reactivity is observed as well.
Immunocytochemistry/Immunofluorescence: MITF Antibody (21D1418) [NB100-56561] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MITF Antibody (21D1418) NB100-56561 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Cells were imaged using a 100X objective and digitally deconvolved.
Western Blot: MITF Antibody (21D1418) [NB100-56561] - Analysis of HeLa lysate (35ug per lane, RIPA buffer) using MiTF antibody (NB100-56561) at 0.1ug/ml. Band detected at ~60kDa. (Expected MW of 58.2kDa according to NP_937802.1).
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Immunocytochemistry/Immunofluorescence: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Staining Tyrosinase in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemistry-Paraffin: OCT6 Antibody [NBP3-13349] - OCT6 antibody detects OCT6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E10.5 embryo. Green: OCT6 stained by OCT6 antibody (NBP3-13349) diluted at 1:500. Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (NBP2-19306) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/Immunofluorescence: OCT6 Antibody [NBP3-13349] - OCT6 antibody detects OCT6 protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: OCT6 stained by OCT6 antibody (NBP3-13349) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.
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Prolactin was detected in immersion fixed paraffin-embedded sections of human testis using Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of sperm cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of human pituitary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(6 Publications)
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NB300-141
Immunohistochemistry: GFAP Antibody [NB300-141] - Analysis of a rat cerebellum section stained with rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green and mouse monoclonal antibody to MeCP2, dilution 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45 uM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astrocytic cells and the processes of Bergmann glia in the molecular layer. The MeCP2 antibody specifically labels nuclei of certain neurons.
Western Blot: GFAP Antibody [NB300-141] - Analysis of different tissue lysates using rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Strong band at about 50 kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.
Western Blot: GFAP Antibody [NB300-141] - Analysis of Rat brain lysate. Antibody at 1:5000. Specific band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to rodent GFAP was observed.
(12 Reviews)
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Western Blot: SRY Antibody (1G4) [NBP2-37463] - Analysis using SRY mAb against human SRY recombinant protein. (Expected MW is 49.4 kDa)
Western Blot: SRY Antibody (1G4) [NBP2-37463] - Analysis using SRY mouse mAb against NTERA-2 (1) cell lysate.
Species: Hu
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SOX10 Antibody
(SD204-04)
NBP2-67812
SOX10 Antibody (SD204-04)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol SOX10