Myelin Protein Zero Antibody

Immunocytochemistry/Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607] - Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. (Green) P0 concentration was 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution). Red is actin. Blue is DAPI nuclear counterstain.

Immunohistochemistry: Myelin Protein Zero Antibody [NB100-1607] - In the image (a lower power tissue section through an adult sciatic nerve), Myelin Protein Zero (brown staining) can be seen in all of the myelinating Schwann cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Myelin Protein Zero Antibody Summary

• Immunogen: Chickens were immunized with two synthetic peptide/keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of Myelin Protein Zero, but are shared between the human (NP_000521, NCBI) and mouse (NP_032649, NCBI) sequences.
• Marker: Schwann Cell Marker
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: MPZ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:2000-1:5000
  • Immunohistochemistry 1:2000-1:5000
  • Immunoprecipitation
  • Western Blot 1:10000-1:20000
• Application Notes: Western Blot reactivity reported in scientific literature (PMID: 22689911). Use in immunoprecipitation reported in scientific literature (PMID:26406915).
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse reactivity reported in scientific literature (PMID: 26406915), Rat reactivity reported in scientific literature (PMID: 22511272).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM PBS (0.9% isotonic, w/v, pH 7.2)
• Preservative: 0.02% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Purification Notes
After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 ug/ml. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Alternate Names for Myelin Protein Zero Antibody

• Charcot-Marie-Tooth neuropathy 1B
• CHM
• CMT1
• CMT1B
• CMT2I
• CMT2J
• CMT4E
• CMTDI3
• HMSNIB
• MPP
• Myelin peripheral protein
• myelin protein P0
• myelin protein zeroDSS
• P0

Background

Myelin Protein Zero encodes a major structural protein of peripheral myelin. Mutations in this gene result in the autosomal dominant form of Charcot-Marie-Tooth disease type 1 as other polyneuropathies. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myelin Protein Zero Antibody (NB100-1607)(14)

Publications using NB100-1607

• Xu J, Zhang B, Cai J et al. The transcription factor Stat-1 is essential for Schwann cell differentiation, myelination and myelin sheath regeneration Molecular medicine (Cambridge, Mass.) 2023-06-26 [PMID: 37365519] (ICC/IF, IHC-P, WB, Rat)
  
  Details:
  1:500 dilution
• Wang G, Yin W, Shin H Et al. Neuronal accumulation of peroxidated lipids promotes demyelination and neurodegeneration through the activation of the microglial NLRP3 inflammasome Nature Aging 2021-11-01 [PMID: 37118341]
• Shen X, Qu F, Pei Y et al. Repairing sciatic nerve injury with self-assembling peptide nanofiber scaffold-containing chitosan conduit Frontiers in Neurology 2022-10-13 [PMID: 36313506]
• Kornelius E, Tsou SH, Chang CC et al. Liraglutide Attenuates Glucolipotoxicity-Induced RSC96 Schwann Cells' Inflammation and Dysfunction Biomolecules 2022-09-21 [PMID: 36291547] (ICC/IF)
• Tseng KY, Wang HC, Cheng KF et al. Sciatic Nerve Intrafascicular Injection Induces Neuropathy by Activating the Matrix Modulators MMP-9 and TIMP-1 Frontiers in pharmacology 2022-05-20 [PMID: 35694244] (IHC-Fr, Rat)
• Mikulka CR, Dearborn JT, Benitez BA et al. Cell-autonomous expression of the acid hydrolase galactocerebrosidase Proceedings of the National Academy of Sciences 2020-04-21 [PMID: 32253319]
• Chen R, Yang X, Zhang B et al. EphA4 Negatively Regulates Myelination by Inhibiting Schwann Cell Differentiation in the Peripheral Nervous System Front Neurosci 2019-11-13 [PMID: 31798398] (ICC/IF, WB, Rat)
• Su W, Gu Y, Wei Z et al. Rab27a/Slp2-a complex is involved in Schwann cell myelination. Neural Regeneration Research. 2016-12-09 [PMID: 28123429] (ICC/IF, Rat)
• Brugger V, Engler S, Pereira JA et al. HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins. PLoS Biol. 2015-09-01 [PMID: 26406915] (IP, ICC/IF, Mouse, Human)
• Martinez-Vega R, Garrido F, Partearroyo T et al. Folic acid deficiency induces premature hearing loss through mechanisms involving cochlear oxidative stress and impairment of homocysteine metabolism. FASEB J. 2014-11-10 [PMID: 25384423]
• Saporta MA, Shy BR, Patzko A et al. MpzR98C arrests Schwann cell development in a mouse model of early-onset Charcot-Marie-Tooth disease type 1B. Brain. 2012-07-01 [PMID: 22689911] (IF/IHC, WB, ICC/IF, Mouse)
  
  Details:
  ATF6 (IMG-273 clone 70B1413.1) WB (mouse sciatic nerves, data described but no shown).
• Hossain S, de la Cruz-Morcillo MA, Sanchez-Prieto R, Almazan G. Mitogen-activated protein kinase p38 regulates krox-20 to direct schwann cell differentiation and peripheral myelination. Glia;60(7):1130-44. 2012-07-01 [PMID: 22511272] (ICC/IF, Rat)
• Asada N, Takase M, Nakamura J et al. Dysfunction of fibroblasts of extrarenal origin underlies renal fibrosis and renal anemia in mice. The Journal of Clinical Investigation. 2011-09-12 [PMID: 21911936]
• Malheiro A Investigating the effect of phosphodiesterase-4 (PDE4) inhibitors on Schwann cells myelination in a 3D regeneration model and in a hyperglycemia model Book 2021-06-02

FAQs for Myelin Protein Zero Antibody (NB100-1607). (Showing 1 - 1 of 1 FAQs).

1. Does this antibody target the extracellular domain of the protein?
   • Two different peptides were used to raise this antibody: 1) the first peptide sequence is located in the amino-terminal half of the protein and is located within an "Immunoglobulin V-type" domain, which is extracellular. 2) The second peptide sequence is located near the C-terminus of the protein (cytoplasmic). The antibody recognizes MPZ protein, when both antigens are available but we do not know how strong would be the signal if only extracellular antigen is visible.

Bioinformatics

• Gene Symbol: MPZ
• Entrez:
  • 4359(Human)
  • 17528(Mouse)
• Uniprot:
  • NP_000521(Human)
  • P25189(Human)
  • NP_032649(Mouse)
  • P27573(Mouse)