Immunocytochemistry/ Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607] - Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of ...read more
Immunohistochemistry: Myelin Protein Zero Antibody [NB100-1607] - In the image (a lower power tissue section through an adult sciatic nerve), Myelin Protein Zero (brown staining) can be seen in all of the myelinating ...read more
Chickens were immunized with two synthetic peptide/keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of Myelin Protein Zero, but are shared between the human (NP_000521, NCBI) and mouse (NP_032649, NCBI) sequences.
Schwann Cell Marker
Immunogen affinity purified
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Both of these anti-peptide antibodies were analyzed by immunohistochemistry (1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500) as the secondary reagent. Western Blot reactivity reported in scientific literature (PMID: 22689911). Use in immunoprecipitation reported in scientific literature (PMID:26406915).
27 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB100-1607 in the following applications:
Human, Mouse reactivity reported in scientific literature (PMID: 26406915), Rat reactivity reported in scientific literature (PMID: 22511272).
Packaging, Storage & Formulations
Store at 4C in the dark.
10mM Sodium PBS (0.9% isotonic, w/v, pH 7.2)
0.02% Sodium Azide
Immunogen affinity purified
Purification Notes After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 ug/ml. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Storage Notes Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Alternate Names for Myelin Protein Zero Antibody
Charcot-Marie-Tooth neuropathy 1B
Myelin peripheral protein
myelin protein P0
myelin protein zeroDSS
Myelin Protein Zero encodes a major structural protein of peripheral myelin. Mutations in this gene result in the autosomal dominant form of Charcot-Marie-Tooth disease type 1 as other polyneuropathies. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Myelin Protein Zero Antibody (NB100-1607). (Showing 1 - 1 of 1 FAQs).
Does this antibody target the extracellular domain of the protein?
Two different peptides were used to raise this antibody: 1) the first peptide sequence is located in the amino-terminal half of the protein and is located within an "Immunoglobulin V-type" domain, which is extracellular. 2) The second peptide sequence is located near the C-terminus of the protein (cytoplasmic). The antibody recognizes MPZ protein, when both antigens are available but we do not know how strong would be the signal if only extracellular antigen is visible.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.