EGR2 Antibody

Images

 
Western Blot: EGR2 Antibody [NB110-59723] - Detection of EGR2 in human fetal lung tissue using NB110-59723.
Immunocytochemistry/ Immunofluorescence: EGR2 Antibody [NB110-59723] - EGR2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunofluorescence: EGR2 Antibody [NB110-59723] - EGR2 staining on E13.5 dorsal root ganglion and neural tube.

Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, ICC/IF, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

EGR2 Antibody Summary

Immunogen
A synthetic peptide made to a portion of human EGR2 (within residues 200-300). [Swiss-Prot# P11161]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EGR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:500
  • Immunofluorescence
Application Notes
This EGR2 antibody is useful for ICC and Western blot, where a band is seen at ~50 kDa. There is also a strong non-specific band at ~75 kDa. In ICC/IF nuclear staining was observed in Hela cells.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Lung Lysate (NB820-59485)
Reviewed Applications
Read 1 Review rated 4
using
NB110-59723 in the following applications:

Reactivity Notes

Human, mouse (review data) and porcine. Immunogen sequence has 86% homology to mouse, rat, chicken and Xenopus proteins.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EGR2 Antibody

  • AT591
  • CMT1D
  • CMT4E
  • early growth response 2 (Krox-20 homolog, Drosophila)
  • early growth response 2
  • early growth response protein 2
  • EGR-2
  • FLJ14547
  • Krox-20 homolog, Drosophila
  • KROX-20, Drosophila, homolog (early growth response-2)
  • KROX20DKFZp686J1957
  • Zinc finger protein Krox-20

Background

EGR2 (E3 SUMO-protein ligase EGR2) protein is a member of EGR C2H2-type zinc-finger family and is a sequence-specific DNA-binding transcription factor that binds to two specific DNA sites located in HOXA4 promotor. EGR2 is an E3 SUMO-protein ligase that facilitates SUMO1 conjugation to its coregulators NAB1/NAB2, whose sumoylation down-regulates EGR2's own transcriptional activity. EGR2 interacts with HCFC1, UBC9 and WWP2, and WWP2 mediated ubiquitinization of EGR2 results in its proteasomal degradation. EGR2 protein activates several genes which are implicated in the formation and maintenance of myelin, the fatty substance that covers and protects nerve cells. EGR2 is considered a "master" transcription factor that regulates PNS myelination and plays a key role in myelin homeostasis. Besided peripheral nerve myelination, EGR2 plays important role in T cell maturation, hindbrain segmentation, and lipid biosynthesis. Genetic alteration of EGR2 results in a variety of relatively severe demyelinating neuropathies such as congenital hypomyelinating neuropathy (CHN), childhood-onset Dejerine-Sottas neuropathy (DSN), and Charcot-Marie-Tooth disease type 1D (CMT1D).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGR2 Antibody (NB110-59723) (0)

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Review for EGR2 Antibody (NB110-59723) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB110-59723:
Filter by Applications
IHC-P
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin EGR2 NB110-59723
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reviewed by:
Daochun Sun
IHC-P Mouse 02/24/2017
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedEmbryonic neural tube,Embryonic dorsal root ganglion
SpeciesMouse

Product General Protocols

View specific protocols for EGR2 Antibody (NB110-59723): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EGR2 Antibody (NB110-59723) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-59723AF405
Alexa Fluor 488 Labeled NB110-59723AF488
Alexa Fluor 647 Labeled NB110-59723AF647
Alexa Fluor 700 Labeled NB110-59723AF700
Biotin Labeled NB110-59723B
DyLight 405 Labeled NB110-59723V
DyLight 488 Labeled NB110-59723G
DyLight 550 Labeled NB110-59723R
DyLight 650 Labeled NB110-59723C
FITC Labeled NB110-59723F
HRP Labeled NB110-59723H

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Daochun Sun
02/24/2017
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol EGR2