Pax3/Pax7 Antibody (274212) [Unconjugated]

Pax3 was detected in immersion fixed B16‑F1 mouse melanoma cell line using Mouse Anti-Human/Mouse Pax3 Monoclonal Antibody (Catalog # MAB2457) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: WB, CyTOF-ready, Flow, ICC/IF
• Clone: 274212
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Pax3/Pax7 Antibody (274212) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Pax3 (isoform Pax3a)
  Met1-Ser215
  Accession # NP_000429
• Specificity: Detects human and mouse Pax3 and Pax7 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Pax1 is observed, approximately 10% with rhPax6, and less than 2% with rhPax2, rhPax4, rhPax5, and rhPax9.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAX3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax3/Pax7 Antibody (274212) [Unconjugated]

• Pax3/Pax7

Background

Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. Human and mouse Pax3 share 98% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pax3/Pax7 Antibody (MAB2457)(12)

Publications using MAB2457

• M Ramirez, Y Badayeva, J Yeung, J Wu, A Abdalla-Wy, E Yang, FANTOM 5 C, B Trost, SW Scherer, D Goldowitz Temporal analysis of enhancers during mouse cerebellar development reveals dynamic and novel regulatory functions Elife, 2022;11(0):. 2022 [PMID: 35942939] (IHC, Mouse)
• L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State Cell Stem Cell, 2020;0(0):. 2020 [PMID: 32783886] (Western Blot, Human)
• L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State Cell Stem Cell, 2020;0(0):. 2020 [PMID: 32783886] (IHC, Human)
• V Sahakyan, E Pozzo, R Duelen, J Deprest, M Sampaolesi Methotrexate and Valproic Acid Affect Early Neurogenesis of Human Amniotic Fluid Stem Cells from Myelomeningocele Stem Cells Int, 2017;2017(0):6101609. 2017 [PMID: 29056972] (ICC, Human)
• Arts N, Cane S, Hennequart M, Lamy J, Bommer G, Van den Eynde B, De Plaen E microRNA-155, induced by interleukin-1ss, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells. PLoS ONE, 2015;10(4):e0122517. 2015 [PMID: 25853464] (Western Blot, Human)
• Abraham J, Nunez-Alvarez Y, Hettmer S, Carrio E, Chen H, Nishijo K, Huang E, Prajapati S, Walker R, Davis S, Rebeles J, Wiebush H, McCleish A, Hampton S, Bjornson C, Brack A, Wagers A, Rando T, Capecchi M, Marini F, Ehler B, Zarzabal L, Goros M, Michalek J, Meltzer P, Langenau D, LeGallo R, Mansoor A, Chen Y, Suelves M, Rubin B, Keller C Lineage of origin in rhabdomyosarcoma informs pharmacological response. Genes Dev, 2014;28(14):1578-91. 2014 [PMID: 25030697] (ICC, Human)
• Kikuchi K, Hettmer S, Aslam M, Michalek J, Laub W, Wilky B, Loeb D, Rubin B, Wagers A, Keller C Cell-cycle dependent expression of a translocation-mediated fusion oncogene mediates checkpoint adaptation in rhabdomyosarcoma. PLoS Genet, 2014;10(1):e1004107. 2014 [PMID: 24453992]
• Plouhinec J, Roche D, Pegoraro C, Figueiredo A, Maczkowiak F, Brunet L, Milet C, Vert J, Pollet N, Harland R, Monsoro-Burq A Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers. Dev Biol, 2014;386(2):461-72. 2014 [PMID: 24360906] (Functional Assay, Human)
• Awaya , Tomonari, Kato , Takeo, Mizuno , Yuta, Chang , Hsi, Niwa , Akira, Umeda , Katsutsu, Nakahata , Tatsutos, Heike , Toshio Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells. PLoS ONE, 2012;7(12):e51638. 2012 [PMID: 23236522] (ICC, Human)
• Magli A, Schnettler E, Rinaldi F, Bremer P, Perlingeiro R Functional dissection of Pax3 in paraxial mesoderm development and myogenesis. Stem Cells, 2013;31(1):59-70. 2013 [PMID: 23081715] (Western Blot, Mouse)
• Gianakopoulos PJ, Mehta V, Voronova A, Cao Y, Yao Z, Coutu J, Wang X, Waddington MS, Tapscott SJ, Skerjanc IS MyoD directly up-regulates premyogenic mesoderm factors during induction of skeletal myogenesis in stem cells. J. Biol. Chem., 2011;286(4):2517-25. 2011 [PMID: 21078671] (Western Blot, Mouse)
• Gang EJ, Bosnakovski D, Simsek T, To K, Perlingeiro RC Pax3 activation promotes the differentiation of mesenchymal stem cells toward the myogenic lineage. Exp. Cell Res., 2008;314(8):1721-33. 2008 [PMID: 18395202] (Western Blot, Mouse)

Review for Pax3/Pax7 Antibody (MAB2457) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: cynthia morimoto WB Other 02/19/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 21078671
• Species: Other

Bioinformatics

• Gene Symbol: PAX3
• Uniprot:
  • NP_000429()