Pax3/Pax7 Antibody (274212) [Unconjugated]

Images

 
Pax3 was detected in immersion fixed B16‑F1 mouse melanoma cell line using Mouse Anti-Human/Mouse Pax3 Monoclonal Antibody (Catalog # MAB2457) at 2 µg/mL for 3 hours at room temperature. Cells were stained ...read more
Characterization of iMPCs during monolayer differentiation. a–e Representative immunostaining of Pax3 (a), Myf5 (b), MyoD (c), and MyoG (d), and corresponding quantification (e) during iMPC expansion. Scale ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, CyTOF-ready, Flow, ICC/IF
Clone
274212
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Pax3/Pax7 Antibody (274212) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Pax3 (isoform Pax3a)
Met1-Ser215
Accession # NP_000429
Specificity
Detects human and mouse Pax3 and Pax7 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Pax1 is observed, approximately 10% with rhPax6, and less than 2% with rhPax2, rhPax4, rhPax5, and rhPax9.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PAX3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB2457 in the following applications:

Publications
Read Publications using
MAB2457 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax3/Pax7 Antibody (274212) [Unconjugated]

  • Pax3/Pax7

Background

Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. Human and mouse Pax3 share 98% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pax3/Pax7 Antibody (MAB2457)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow Cytometry, Functional Assay, ICC, IHC, Western Blot.


Filter By Application
Flow Cytometry
(1)
Functional Assay
(1)
ICC
(5)
IHC
(3)
Western Blot
(4)
All Applications
Filter By Species
Human
(8)
Mouse
(7)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using MAB2457 Applications Species
M Ramirez, Y Badayeva, J Yeung, J Wu, A Abdalla-Wy, E Yang, FANTOM 5 C, B Trost, SW Scherer, D Goldowitz Temporal analysis of enhancers during mouse cerebellar development reveals dynamic and novel regulatory functions Elife, 2022-08-09;11(0):. 2022-08-09 [PMID: 35942939] (IHC, Mouse) IHC Mouse
L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State Cell Stem Cell, 2020-08-11;0(0):. 2020-08-11 [PMID: 32783886] (Flow Cytometry, ICC, IHC, Human) Flow Cytometry, ICC, IHC Human
V Sahakyan, E Pozzo, R Duelen, J Deprest, M Sampaolesi Methotrexate and Valproic Acid Affect Early Neurogenesis of Human Amniotic Fluid Stem Cells from Myelomeningocele Stem Cells Int, 2017-09-13;2017(0):6101609. 2017-09-13 [PMID: 29056972] (ICC, Human) ICC Human
Arts N, Cane S, Hennequart M, Lamy J, Bommer G, Van den Eynde B, De Plaen E microRNA-155, induced by interleukin-1ss, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells. PLoS ONE, 2015-04-08;10(4):e0122517. 2015-04-08 [PMID: 25853464] (Western Blot, Human) Western Blot Human
Shelton M, Metz J, Liu J, Carpenedo R, Demers S, Stanford W, Skerjanc I Derivation and expansion of PAX7-positive muscle progenitors from human and mouse embryonic stem cells. Stem Cell Reports, 2014-08-07;3(3):516-29. 2014-08-07 [PMID: 25241748] (IHC, Human, Mouse) IHC Human, Mouse
Abraham J, Nunez-Alvarez Y, Hettmer S, Carrio E, Chen H, Nishijo K, Huang E, Prajapati S, Walker R, Davis S, Rebeles J, Wiebush H, McCleish A, Hampton S, Bjornson C, Brack A, Wagers A, Rando T, Capecchi M, Marini F, Ehler B, Zarzabal L, Goros M, Michalek J, Meltzer P, Langenau D, LeGallo R, Mansoor A, Chen Y, Suelves M, Rubin B, Keller C Lineage of origin in rhabdomyosarcoma informs pharmacological response. Genes Dev, 2014-07-15;28(14):1578-91. 2014-07-15 [PMID: 25030697] (ICC, Human, Mouse) ICC Human, Mouse
Kikuchi K, Hettmer S, Aslam M, Michalek J, Laub W, Wilky B, Loeb D, Rubin B, Wagers A, Keller C Cell-cycle dependent expression of a translocation-mediated fusion oncogene mediates checkpoint adaptation in rhabdomyosarcoma. PLoS Genet, 2014-01-16;10(1):e1004107. 2014-01-16 [PMID: 24453992] (ICC, Human, Mouse) ICC Human, Mouse
Plouhinec J, Roche D, Pegoraro C, Figueiredo A, Maczkowiak F, Brunet L, Milet C, Vert J, Pollet N, Harland R, Monsoro-Burq A Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers. Dev Biol, 2013-12-17;386(2):461-72. 2013-12-17 [PMID: 24360906] (Functional Assay, Human) Functional Assay Human
Awaya , Tomonari, Kato , Takeo, Mizuno , Yuta, Chang , Hsi, Niwa , Akira, Umeda , Katsutsu, Nakahata , Tatsutos, Heike , Toshio Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells. PLoS ONE, 2012-12-07;7(12):e51638. 2012-12-07 [PMID: 23236522] (ICC, Human) ICC Human
Magli A, Schnettler E, Rinaldi F, Bremer P, Perlingeiro R Functional dissection of Pax3 in paraxial mesoderm development and myogenesis. Stem Cells, 2013-01-01;31(1):59-70. 2013-01-01 [PMID: 23081715] (Western Blot, Mouse) Western Blot Mouse
Show All 12 Publications.

Review for Pax3/Pax7 Antibody (MAB2457) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using MAB2457:
Filter by Applications
WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Other 02/19/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 21078671
SpeciesOther

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Verified Customer
02/19/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol PAX3
Uniprot